A test strip for rapid detection of glycosylated hemoglobin
A technology for glycosylated hemoglobin and test strips, which is applied to the field of test strips for rapid quantitative detection of glycosylated hemoglobin in blood, can solve the problems of long elution time and complicated operation, and achieves the effects of simple preparation method, simple operation and convenient testing.
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Embodiment 1
[0022] Get cellulose acetate film, cut and grow to be 240mm, width is 9mm, as sample pad 201; Get the PVC polyester film that thickness is 0.35mm, it is cut and grow to be 240mm, and width is 7mm, and it is punched evenly hole, the diameter of which is 3 mm, as the substrate 202; take the polyethylene fiber membrane, soak it in a mixed solution of concentrated sulfuric acid and concentrated nitric acid with a volume ratio of 1:1, and treat it at 40°C for 4 hours, take it out, soak it in purified water, And add sodium hydroxide solution to adjust the pH value to 6.5, take out the polyethylene fiber membrane, place it in 10 mL of a mixed solution containing 5 mM EDC and 5 mM NHS, and react for 0.5 h, then add 5 mM aminophenylboronic acid, and react for 2 h, and the boric acid The group is modified to the surface of the polyethylene fiber membrane, the polyethylene fiber membrane is taken out, and then soaked in a solution containing purified water and 5mM glycine for sealing, the...
Embodiment 2
[0024] Take cellulose acetate film, cut into 240mm, width is 9mm, as sample pad 201; The width is 7mm, and it is uniformly punched, and the hole diameter is 3mm, as the substrate 202; Take the polypropylene cellulose membrane, soak it in the mixed solution of concentrated sulfuric acid and concentrated nitric acid with a volume ratio of 3:1 at 50 Treat at ℃ for 2 hours, take it out, soak in purified water, and add sodium hydroxide solution to adjust the pH value to 6.5, take out the polypropylene cellulose membrane, place it in 10mL of a mixed solution containing 5mM EDC and 5mM NHS to react for 0.5h, and then Add 20mM aminophenylboronic acid, react for 1h, modify the boric acid group to the surface of the polypropylene cellulose membrane, take out the polypropylene cellulose membrane, then soak it in a solution containing purified water and 20mM glycine for blocking, take out the polypropylene cellulose film, drying, cutting and growing into 240mm and 10mm in width as the rea...
Embodiment 3
[0026]Get the cellulose acetate film, cut and grow into 240mm, width is 9mm, as sample pad 201; Get the PC polyester film that thickness is 0.35mm, cut it and grow into 240mm, width is 7mm, and it is evenly punched hole, the diameter of which is 3mm, as the substrate 202; take the nylon membrane, soak it in a mixed solution of concentrated sulfuric acid and concentrated nitric acid with a volume ratio of 6:1, and treat it at 80°C for 0.5h, take it out, soak it in purified water, and Add sodium hydroxide solution to adjust the pH value to 6.5, take out the nylon membrane, place it in 10 mL of a mixed solution containing 5 mM EDC and 5 mM NHS, and react for 0.5 h, then add 40 mM aminophenylboronic acid, and react for 0.5 h, and the boronic acid group Modified to the surface of the nylon membrane, took out the nylon membrane, and then soaked it in a solution containing purified water and 40mM bovine serum albumin to seal it, took out the nylon membrane, dried it, cut it into a len...
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Abstract
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