Kit for fast detecting imazethapyr content of crops

A technology for detecting imazethapyr and imazethapyr, which is applied in the field of enzyme-linked immunosorbent assay kits for detecting imazethapyr, and can solve the problems of complex processing process and expensive equipment

Inactive Publication Date: 2016-05-11
JIANGSU WISE SCI & TECH DEV
View PDF1 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the determination methods of imazethapyr mainly include high performance liquid chromatography, gas chromatography, etc. The detection results are accurate and reliable, but the equipment is relatively expensive, and the sample pretreatment process is relatively complicated

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Kit for fast detecting imazethapyr content of crops

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0027] Preparation of imazethapyr protein conjugates:

[0028] Adopt the succinic anhydride method to obtain imazethapyr hapten derivatives with carboxyl groups, then take 0.05mmol and carrier protein BSA and mix them in 0.05mol / L carbonate buffer solution (CBS) of pH9.6 according to the binding ratio of 10:1, and then add 0.15mmol carbodiimide, stirred at room temperature for 24 hours, and finally dialyzed in 0.2mol / L PBS buffer solution of pH 7.6 for two days to remove unreacted haptens, and stored the obtained protein conjugate solution at -20°C spare.

[0029] Preparation of imazethapyr antibody:

[0030] Select healthy adult purebred BALA / C mice, take 50 μg of immune antigen prepared by coupling with protein and mix with the same amount of complete Freund's adjuvant, and use intraperitoneal injection for initial immunization, and then use the same dose of immune antigen plus the same amount every 3 weeks Incomplete Freund's adjuvant was used for the second and third imm...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a preparation method and a detection method of an ELISA kit for detecting imazethapyr. The kit has the advantages of detection sensitivity, detection accuracy, fastness, operation simpleness and good specificity and is suitable for batch sample detection. The kit for detecting imazethapyr has a detection principle of a solid phase indirect competition ELISA reaction. The detection method comprises adding an extracted sample, an enzyme-labeled second antibody work liquid and an antibody work liquid into corresponding enzyme-labeled holes, carrying out incubation for some time, carrying out plate washing, adding a substrate A and a substrate B into the plate so that the solutions in the enzyme-labeled holes produce a blue color under enzyme action and adding a stopping solution into the enzyme-labeled holes so that the blue color is changed into a yellow color. The color depth of the standard substance or sample is inversely proportional to imazethapyr content of the standard substance or sample. The detection method can be directly used for detecting imazethapyr content of crops (such as leguminous crops).

Description

technical field [0001] The invention relates to the technical field of ELISA detection, in particular to an ELISA kit for detecting imazethapyr. Background technique [0002] Imazethapyr is an imidazolinone herbicide and an inhibitor of side chain amino acid synthesis. It has excellent control effects on gramineous weeds and certain broad-leaved weeds in soybean fields and other legume fields. At present, the determination methods of imazethapyr mainly include high performance liquid chromatography, gas chromatography, etc. The detection results are accurate and reliable, but the instruments and equipment are relatively expensive, and the sample pretreatment process is relatively complicated. Enzyme-linked immunosorbent assay is an accurate, reliable, rapid and specific detection method, suitable for rapid screening of a large number of samples, and has been widely used in the agricultural product safety testing industry in recent years. The invention aims to establish an E...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N33/00
Inventor 洪霞戴蔚蔚
Owner JIANGSU WISE SCI & TECH DEV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap