Method for identifying shellfish germplasm through combination of morphology with molecular biology

Abstract

The invention relates to the technical field of identification of species of mariculture shellfish, in particular to a method for identifying species of shellfish samples by using morphology and molecular biology technologies. According to the method, shellfish are identified with a method combining a morphology method with a molecular biology method, the shellfish samples having certain morphological characteristics are accurately identified with the molecular biology method, morphological characteristics applicable to species identification are summarized, a morphological characteristic retrieve table is established, then species of other samples are identified by the aid of the morphological characteristic retrieve table, and verification is performed with the molecular biology method, so that the identification accuracy of shellfish germplasm resources can be significantly improved, and multiple difficulties in previous species identification are eliminated.

Description

technical field [0001] The invention relates to the technical field of species identification of marine cultured shellfish, in particular to a method for identifying species of shellfish samples by using morphological and molecular genetics techniques. Background technique [0002] Through the investigation and research of shellfish resources, data such as the number, type and distribution of shellfish can be obtained, which is of great significance to the protection and rational development and utilization of shellfish resources. Accurate identification of shellfish species is the basis for research on shellfish germplasm resources. [0003] The existing information on shellfish species identification and morphological characteristics in my country is mainly through collecting shellfish in the wild and using morphological characteristics to determine the species. Usually, shellfish of different species in the same family have similar appearance characteristics in the same ...

AI Technical Summary

Problems solved by technology

However, due to the chemical modification of formalin on DNA molecules, it will bring great difficulties to the DNA extraction and amplification of samples, so 70% ethanol is usually used to fix and store

However, the strong dehydration effect of ethanol will cause the sample to shrink and harden, and the morphology will change significantly, which is not conducive to morphological observation.

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