Establishment method of bone marrow suppression mouse model

A mouse model, myelosuppression technology, applied in the medical field, can solve the problems of poor reproducibility and low molding rate of myelosuppression animal models

Inactive Publication Date: 2016-05-25
SHANGHAI HOSPITAL OF TRADITIONAL CHINESE MEDICINE
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Problems solved by technology

[0004] The purpose of the present invention is to provide a method for establishing a mouse model of bone marrow suppression with high modeling rate

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  • Establishment method of bone marrow suppression mouse model
  • Establishment method of bone marrow suppression mouse model
  • Establishment method of bone marrow suppression mouse model

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Embodiment Construction

[0068] The present invention will be further described below in combination with specific embodiments. The following examples can enable those skilled in the art to understand the present invention more fully.

[0069] The establishment method of embodiment myelosuppression mouse model

[0070] 1 Experimental animals and grouping: 10 6-week-old NOD / SCID male mice, weighing (23±4) g, SPF grade, were provided by Shanghai Slack Experimental Animal Co., Ltd., and were raised in the Experimental Animal Center of Shanghai University of Traditional Chinese Medicine. At room temperature (27±1)°C, eat full-price nutritional pellet feed. After adaptive feeding for 3 days, they were randomly divided into 2 groups (control group and model group), with 5 rats in each group.

[0071] 2. Dosing method: Dosing is based on the initial body weight, and the experiment has been continuously observed for 15 days since the administration began.

[0072] Control group: intraperitoneal injection o...

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Abstract

The invention belongs to the technical field of medical science, and particularly relates to an establishment method of a bone marrow suppression mouse model. The problems that at present, a bone marrow suppression animal model is low in modeling rate and poor in repeatability are solved. The method comprises the steps that an NOD or SCID mouse is adopted, the abdominal cavity of the mouse is injected with 150 mg/kg, calculated according to the initial body weight, of cyclophosphamide, injection is conducted every other day, injection is conducted three times in total, the observation period is 15 days, and the bone marrow suppression mouse model can be obtained. According to the method for establishing the bone marrow suppression mouse model, operation is easy, the modeling rate is high, the repeatability is good, the method can be used for various experimental animals, a foundation is laid for research on bone marrow suppression experiments, the bone marrow suppression experiments and clinical application research can be more normalized and standardized, objective and accurate verification on drug efficacy and action mechanisms of various drugs for preventing and treating bone marrow suppression is facilitated, further guidance on selection and evaluation of clinical treatment schemes is facilitated, and guidance on pharmacology experiments and development of new drugs for treating bone marrow suppression is facilitated.

Description

technical field [0001] The invention belongs to the field of medical technology. It specifically relates to a method for establishing a mouse model of bone marrow suppression. Background technique [0002] Bone marrow suppression is a common adverse reaction of most chemotherapy and radiotherapy drugs, manifested as changes in the hematopoietic system such as peripheral blood single-lineage or whole-lineage cytopenia. There have been many reports on the myelosuppression caused by cyclophosphamide, but there is no uniform standard for the use of cyclophosphamide to reproduce the myelosuppression model, and the dosage and time of cyclophosphamide are still controversial, resulting in a low modeling rate of myelosuppression animal models , Reproducibility is poor, thus affecting its pathogenesis and efficacy of drug prevention and treatment of myelosuppression. [0003] How to establish a clear and reproducible animal model of myelosuppression is crucial to the in-depth study...

Claims

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Application Information

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IPC IPC(8): A01K67/02
CPCA01K67/02
Inventor 姚重华刘丽琼苏晓杨旭鸣黄慧萍江春春赵文修王洁高龙刘军成
Owner SHANGHAI HOSPITAL OF TRADITIONAL CHINESE MEDICINE
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