Traditional herbal compound for resisting porcine reproductive and respiratory syndrome, extraction method and concentration and effect determination

Abstract

The invention discloses a traditional herbal compound for resisting the porcine reproductive and respiratory syndrome, an extraction method, concentration and effect determination. The traditional herbal compound is prepared from, by weight, 3 parts of sweet wormwood herb, 2 parts of common andrographis herb, 2 parts of isatis roots and 1 part of roots of common peony. The extraction method comprises the steps that soaking with water is performed for 1 h, the addition amount of water for soaking is four times the weight of the traditional herbal compound, then, water with the amount four times the weight of the traditional herbal compound is added, decoction is performed for 20-30 min, and filtering is performed to obtain a first-time decocted solution; then, water with the amount five times the weight of the traditional herbal compound is added, decoction is performed for 20 min, and filtering is performed to obtain a second-time decocted solution; the first-time decocted solution and the second-time decocted solution are combined and heated and decocted in a beaker, the volume is metered to reach 20 ml, and 0.4 g of crude drug exists per milliliter; cooling is performed, a centrifugal machine is used for performing centrifuging for 10 min at the rotating speed of 3000 r / min, a supernatant is taken to be stored in a 200 ml volumetric flask, a NaHCO3 solution is used for adjusting the pH value to reach 7.0, autoclaved sterilization and packaging are performed to be stored under the temperature of 4 DEG C for use. The traditional herbal compound has the effects of supporting body resistance, adjusting the body equilibrium between yin and yang, boosting immunity, clearing away heat and toxic materials, promoting blood circulation to remove blood stasis, strengthening the foundation, relieving exterior syndrome and the like, and can affect virus reproduction directly or by adjusting immunity of the organism.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine for veterinary use, and relates to a traditional Chinese medicine compound for in vitro anti-pig PRRS virus, an extraction method, concentration and effect measurement. Background technique [0002] PRRS (also known as porcine reproductive and respiratory syndrome) is a highly contagious disease of pigs mainly characterized by reproductive impairment and respiratory symptoms caused by porcine reproductive and respiratory syndrome virus (PRRSV). The disease can be transmitted vertically and horizontally, and can cause fever, anorexia, abortion, premature birth, mummified fetuses, weak piglets, and various respiratory diseases in piglets and piglets in pregnant sows. In 1996, Guo Baoqing and others confirmed its existence in my country. PRRS has the characteristics of rapid spread, widespread prevalence and strong strain mutation ability. There is no effective drug yet, and its comprehens...

AI Technical Summary

Problems solved by technology

In 1996, Guo Baoqing and others confirmed its existence in my country. PRRS has the characteristics of rapid spread, widespread prevalence and strong strain mutation ability. There is no effective drug yet, and its comprehensive prevention and control is very difficult

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