A kind of preparation method of goat keratin antibody
A technology of goat horn and keratin, which is applied in the direction of immunoglobulin, chemical instruments and methods, and immunoglobulin from serum, etc., can solve the problems of difficult identification of goat hair products, and achieve low critical micelle concentration, high sensitivity, and reaction The effect of high sensitivity
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Embodiment 1
[0028] A preparation method of goat keratin antibody, the steps are as follows:
[0029] A) Prepare a 7wt% aqueous solution of potassium hydrogensulfate, and adjust the pH of the aqueous solution of potassium hydrogensulfate to 4 with saturated sodium hydroxide solution. Then goat hair was added to the solution at a bath ratio of 1:58, and shaken in a water bath at 32° C. for 1 h. Then the wool was taken out and washed and filtered with distilled water until the pH value of the washing liquid was 7. The wool was added to the mixed solution A which was 80 times its mass. The concentration of sodium bisulfite in the mixed solution A was 6wt%, the concentration of urea was 6M, and the concentration of sodium dodecylbenzenesulfonate was 1wt%. React at 100°C for 4 hours and then filter to obtain a crude keratin solution. Put the crude keratin solution into a dialysis bag with a molecular weight cut-off of 14,000, soak the dialysis bag in 50-100 times the mass of deionized water an...
Embodiment 2
[0036] A preparation method of goat keratin antibody, the steps are as follows:
[0037] A) Prepare a 7wt% aqueous solution of potassium hydrogensulfate, and adjust the pH of the aqueous solution of potassium hydrogensulfate to 4.2 with saturated sodium hydroxide solution. Then goat hair was added to the solution at a bath ratio of 1:57, and shaken in a water bath at 33° C. for 1 h. Then the wool was taken out and washed and filtered with distilled water until the pH value of the washing liquid was 7. Wool is added in the mixed solution A of its 80 times of mass, the concentration of sodium bisulfite in described mixed solution A is 6wt%, the concentration of urea is 6M, the concentration of saponin is 0.5wt%, dodecylbenzene The concentration of sodium sulfonate was 0.8 wt%. React at 100°C for 4 hours and then filter to obtain a crude keratin solution. Put the crude keratin solution into a dialysis bag with a molecular weight cut-off of 14,000, soak the dialysis bag in 50-10...
Embodiment 3
[0042] A preparation method of goat keratin antibody, the steps are as follows:
[0043] A) Prepare a 6wt% aqueous solution of potassium hydrogensulfate, and adjust the pH of the aqueous solution of potassium hydrogensulfate to 3.5 with saturated sodium hydroxide solution. Then goat hair was added to the solution at a bath ratio of 1:50, and shaken in a water bath at 30°C for 1.5h. Then the wool was taken out and washed and filtered with distilled water until the pH value of the washing liquid was 7. Wool is added in the mixed solution A of its 60 times of mass, the concentration of sodium bisulfite in described mixed solution A is 6wt%, the concentration of urea is 6M, the concentration of saponin is 0.5wt%, dodecylbenzene The concentration of sodium sulfonate was 0.8 wt%. After reacting at 95°C for 4.5 hours, filter to obtain a crude keratin solution, put the crude keratin solution into a dialysis bag with a molecular weight cut-off of 14,000, soak the dialysis bag in deio...
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