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Method for indirectly preparing D-sodium erythorbate

An indirect technology of sodium erythorbate, applied in the field of indirect preparation of sodium D-erythorbate, can solve the problems of low yield and purity of sodium D-erythorbate, achieve good promotion and application prospects, high conversion rate, and high purity of crude products Effect

Active Publication Date: 2016-05-25
SHANDONG FOOD & FERMENT IND RES & DESIGN INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, 2-KGA production bacteria at home and abroad usually use the strains of the genus Pseudomonas, and the yield and purity of D-sodium erythorbate obtained from the fermentation liquid of this strain after esterification and other treatments are relatively low

Method used

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  • Method for indirectly preparing D-sodium erythorbate

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Experimental program
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Effect test

Embodiment 1

[0037] The preparation method of indirect preparation D-sodium erythorbate, concrete steps are:

[0038] 1) Strain fermentation

[0039] The Serratia marcescens (Serratia marcescens) CGMCCN0.10548 strain was transplanted to the slant for activation.

[0040] Prepare seed medium according to the composition of the above-mentioned liquid seed medium. The liquid volume in a 500ml triangular bottle is 50ml, steam sterilize at 121°C for 20 minutes, cool to room temperature, inoculate 2 rings of slant strains, set the rotation speed to 200r / min, and the radius of rotation Incubate on a 40mm shaker at 30°C for 12 hours to obtain a seed liquid, which is taken at 600 nm to measure the absorbance value to 0.262;

[0041] Prepare the fermentation medium in a 5L fermenter according to the liquid volume of 3L, adjust the pH to 7.0, sterilize the tank at 115°C for 20 minutes, cool the circulating water to 30°C, inoculate 300ml of the cultured seed liquid, and take a sample immediately afte...

Embodiment 2

[0054] The preparation method of indirect preparation D-sodium erythorbate, concrete steps are:

[0055] 1) Strain fermentation

[0056] The Serratia marcescens (Serratia marcescens) CGMCCN0.10548 strain was transplanted to the slant for activation.

[0057] Prepare seed medium according to the composition of the above-mentioned liquid seed medium. The liquid volume in a 500ml triangular bottle is 50ml, steam sterilize at 121°C for 20 minutes, cool to room temperature, inoculate 2 rings of slant strains, set the rotation speed to 200r / min, and the radius of rotation Cultivate on a 40mm shaker at 30°C for 12 hours to obtain a seed liquid, and take the seed liquid at 600 nm to measure the absorbance value to 0.265;

[0058] Use a 10L fermenter to prepare a fermentation medium with a liquid volume of 7L, adjust the pH to 7.0, sterilize the tank at 115°C for 20 minutes, cool the circulating water to 30°C, inoculate 700ml of the cultured seed liquid, and take a sample immediately ...

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Abstract

The invention discloses a method for indirectly preparing D-sodium erythorbate, belonging to the technical field of biochemical separation synthesis. The method comprises the following steps: by using a Serratia marcescens SDSPY-136 strain fermentation liquid as a raw material, carrying out fermentation liquid pretreatment, methyl 2-keto-D-gluconate reaction and conversion reaction to obtain crude D-sodium erythorbate; and carrying out refinement and purification to obtain the D-sodium erythorbate product. The Serratia marcescens is used as a production strain to obtain the calcium 2-keto-D-gluconate, and multiple impurity removal modes are combined to convert the treatment liquid into the D-sodium erythorbate. The obtained fermentation liquid has the advantages of high 2-keto-D-gluconic acid yield and high conversion rate; the crude D-sodium erythorbate product has high purity and is easy to refine, so the total yield is enhanced; and the method has favorable popularization and application prospects.

Description

technical field [0001] The invention relates to the technical field of biochemical separation and synthesis, in particular to a method for indirectly preparing sodium D-isoascorbate. Background technique [0002] D-Erythorbicacid (EA), also known as erythorbic acid or arabic ascorbic acid, is an optical isomer of sodium L-ascorbate, referred to as iso-Vc. D-isoascorbic acid and its sodium salt (Sodiumerythorbate, EN) have strong reducing properties, antiseptic and anti-oxidant effects are much better than vitamin C, and their heat stability is also stronger than vitamin C, but the price is only vitamin C 1 / 2 of. Erythorbic acid and its sodium salt are safe and effective food additives, widely used in various foods. Sodium erythorbate is white or yellowish needle-like crystal, odorless, slightly salty taste, relatively stable in the air, gradually darkens when exposed to light, easily soluble in water, slightly soluble in methanol and ethanol. The preparation methods of so...

Claims

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Application Information

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IPC IPC(8): C12P17/04C12R1/42
CPCC12P17/04
Inventor 徐慧刘建军李文婧李博孙文涛刘丽萍
Owner SHANDONG FOOD & FERMENT IND RES & DESIGN INST
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