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Preparing method for ginsenoside Rh2

A technology of ginsenoside and production method, which is applied in the field of medicine, can solve the problems of low yield, many by-products, and environmental pollution in chemical methods, and achieve the effects of high purity, few by-products, and high selectivity

Inactive Publication Date: 2016-05-25
杭州双马生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Poor effect of prior art on separating ginsenoside Rh2
In the past, chemical methods such as acid or alkali hydrolysis were often used, but chemical methods had disadvantages such as low yield, many by-products, and environmental pollution.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The production method of ginsenoside Rh2 comprises the following steps,

[0027] A. In aseptic condition, inoculate Lactobacillus bulgaricus on the medium, and control the temperature at 37°C for anaerobic culture for 24 hours;

[0028] The cultured Lactobacillus bulgaricus strains were inoculated with the Lactobacillus bulgaricus strains, cultured in an anaerobic incubator for 24 hours, and then expanded twice;

[0029] B. Select the ginseng whose surface is grayish yellow, grind the ginseng into ginseng powder, and the impurities in the powder shall not exceed 1%;

[0030] C. Add ginseng powder and water into the fermenter, add 0.8kg ginseng powder to 5L of water, and start stirring; open the steam valve and feed steam into the interlayer of the tank. At 121°C, turn off the agitation, control the interlayer pressure to 0.1mpa, and insulate and sterilize for 30 minutes;

[0031] After the sterilization is finished, close the steam inlet valve of the interlayer, empty...

Embodiment 2

[0042] The production method of ginsenoside Rh2 comprises the following steps,

[0043] A. In aseptic condition, inoculate Lactobacillus bulgaricus on the medium, and control the temperature at 37°C for anaerobic culture for 24 hours;

[0044] The cultured Lactobacillus bulgaricus strains were inoculated with the Lactobacillus bulgaricus strains, cultured in an anaerobic incubator for 24 hours, and then expanded twice;

[0045] B. Select the ginseng whose surface is grayish yellow, grind the ginseng into ginseng powder, and the impurities in the powder shall not exceed 1%;

[0046] C. Add ginseng powder and water into the fermentation tank, add 1.5kg ginseng powder into 5L of water, and start stirring; open the steam valve and feed steam into the interlayer of the tank body, the steam will enter from the upper part and discharge from the lower part, when the temperature of the feed liquid rises to At 121°C, turn off the agitation, control the interlayer pressure to 0.12mpa, a...

Embodiment 3

[0058] The production method of ginsenoside Rh2 comprises the following steps,

[0059] A. In aseptic condition, inoculate Lactobacillus bulgaricus on the medium, and control the temperature at 37°C for anaerobic culture for 24 hours;

[0060] The cultured Lactobacillus bulgaricus strains were inoculated with the Lactobacillus bulgaricus strains, cultured in an anaerobic incubator for 24 hours, and then expanded twice;

[0061] B. Select the ginseng whose surface is grayish yellow, grind the ginseng into ginseng powder, and the impurities in the powder shall not exceed 1%;

[0062] C. Add ginseng powder and water into the fermentation tank, add 1kg ginseng powder into 5L of water, and start stirring; open the steam valve and feed steam into the interlayer of the tank body. At ℃, turn off the agitation, control the interlayer pressure to 0.11mpa, and insulate and sterilize for 30 minutes;

[0063] After the sterilization is finished, close the steam inlet valve of the interla...

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PUM

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Abstract

The invention relates to the technical field of medicine and discloses a preparing method for ginsenoside Rh2. The preparing method comprises the steps of strain culture, raw material selecting, biological fermentation and conversion, filter pressing, sterilization and drying, and thus a product is obtained. Ginsenoside Rh2 extract prepared through the preparing method is high in treatment effect and purity, good in absorption and stable in quality; meanwhile, the preparing method is good in separation effect, high in content, simple in process, convenient to implement, low in cost and suitable for industrial production.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to a production method of ginsenoside Rh2. Background technique [0002] Ginsenoside Rh2 is a secondary saponin produced by hydrolysis of ginsenosides. Pharmacodynamic studies have shown that ginsenoside Rh2 has a significant and specific inhibitory effect on the proliferation of cancer cells such as liver cancer, melanoma B16, cervical cancer and sarcoma S180. Its mechanism of action is as follows: in the process of cancer cell proliferation, through the action of ginsenoside Rh2, the cancer cells stop at a certain proliferation stage and promote their reversion to normal cells. The effect of the prior art on separating ginsenoside Rh2 is not good. In the past, chemical methods such as acid or alkali hydrolysis were often used, but chemical methods had disadvantages such as low yield, many by-products, and environmental pollution. Contents of the invention [0003] Aiming at t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P33/20C12R1/225
CPCC12P33/20
Inventor 冯建平常勇进
Owner 杭州双马生物科技股份有限公司
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