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Method for identifying acaudina molpadioides by using specific peptide fragment group

A Haitian melon, specific technology, applied in the biological field, can solve the problems of lack of technical level, deceiving consumers, etc.

Active Publication Date: 2016-05-25
INSPECTION & QUARANTINE TECH CENT SHANDONG ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Due to the lack of a particularly effective method of identifying sea cucumber species at present, the method of identifying sea cucumber species is only based on traditional appearance observation, lacking technical support, resulting in many unscrupulous merchants cheating consumers by shoddy goods and false ones. Therefore, it is necessary to An efficient and accurate method for identifying sea cucumbers

Method used

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  • Method for identifying acaudina molpadioides by using specific peptide fragment group
  • Method for identifying acaudina molpadioides by using specific peptide fragment group
  • Method for identifying acaudina molpadioides by using specific peptide fragment group

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1, Sequence Source and Alignment Information of Specific Polypeptides

[0040] 1. Haitian melon's unique source of peptides VMTDEMER, FNENEEMYEK, FNENEEMYETK, MSELESQVVSSK

[0041] The unique polypeptides VMTDEMER, FNENEEMYEK, FNENEEMYETK, and MSELESQVVSSK of Haitian melon all come from the protein tropomyosin [Apostichopus japonicus], and its Accesionnumber on NCBI is gi|302340969, and its sequence is shown in SEQ ID NO.6 below. The analysis found that the first Glu mutation of EMTEEMER It became Val, the 4th Glu was mutated into Asp, and the polypeptide VMTDEMER was obtained; the 2nd Thr of FTENEEMYEK was mutated into Asn, and the polypeptide FNENEEMYEK was mutated; the 2nd Thr of FTENEEMYEKK was mutated into Asn, and the 10th Lys Mutated into Thr, the polypeptide FNENEEMYETK was obtained; the 6th Thr of MSELETQVMSSK was mutated into Ser, and the 9th Met was mutated into Val, and the polypeptide MSELESQVVSSK was obtained; after NCBIblast comparison, the above ...

Embodiment 2

[0069] Embodiment 2, sea cucumber sample processing and detection steps

[0070] The steps of analyzing the sea cucumber samples to be tested include

[0071] (1) Sample pretreatment steps:

[0072] (1) Weigh 1g sea cucumber sample and homogenize it into a powder state, add 10mL protein extract (8M urea, 50mM NH 4 HCO 3 ) shake to extract protein, centrifuge at high speed and low temperature (20000r / min), take 200ul of supernatant and transfer to 1mlEP tube;

[0073] (2) Add 2 μL of 1mol / LDTT (0.154 g of dithiothreitol DTT dissolved in 1 mL of deionized water) to 100 μL of the above protein solution and react at 37°C for 1 hour;

[0074] (3) Take 10 μL of freshly prepared IAA (accurately weigh 0.185g of iodoacetamide IAA and dissolve it in 1mL of deionized water to prepare a 1mol / L IAA mother solution for later use. The preparation process needs to be protected from light, and it is ready to use) and added to In the above reaction solution that has been cooled to room temp...

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Abstract

The invention relates to a method for identifying acaudina molpadioides by using a specific peptide fragment group, and a group of comparison polypeptides used for identifying the acaudina molpadioides with a mass-spectrography method. A sequence of each polypeptide is SEQ ID NO.1-.5 and is not expressed in other species.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for identifying Haitian melons by using specific peptide groups. Background technique [0002] Sea cucumbers are rich in acidic mucopolysaccharides, which have the effects of immunomodulators, anticoagulants, blood sugar control and blood fat reduction on the human body. Therefore, sea cucumber enjoys the reputation of "treasure house of nutrition", and modern people regard sea cucumber as "the first of the eight delicacies of seafood". There are many kinds of sea cucumbers in the market, and the prices of different sea cucumbers vary greatly due to their nutritional value and taste. Acaudinamolpadioides is an echinoderm of the taro sea cucumber family, commonly known as fragrant ginseng, also known as white ginseng and sea eggplant. Haitian melon is very nutritious, but this marine resource has not been fully exploited so far. Sea cucumber is the only animal f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/62
CPCG01N27/62
Inventor 张鸿伟张晓梅王凤美徐彪
Owner INSPECTION & QUARANTINE TECH CENT SHANDONG ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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