A method for fast propagation and seedling growth of magnolia fadou tissue

A technology for rapid propagation of seedlings and tissues, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve problems such as restrictions on the development and utilization of magnolia magnolia, lack of seedling raising measures, rapid propagation technology and management methods, and difficulty in seed collection. , to achieve the effect of short cultivation period, high induction rate, and strong rooted seedlings

Active Publication Date: 2017-09-05
SOUTH CHINA AGRI UNIV
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Problems solved by technology

Coupled with the difficulty of seed collection, the lack of effective seedling raising measures, rapid propagation techniques and management methods has seriously restricted the development and utilization of Fadouma magnolia.
[0005] According to the literature search, there is no report about the tissue culture and rapid propagation of Manglotticus fadou at present, and there is only one article (Study on growth characteristics and light and physiology of 4 species of Magnolia magnolia seedlings, 2014, Zhu Xianliang, etc.). Dou Mulian

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  • A method for fast propagation and seedling growth of magnolia fadou tissue
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  • A method for fast propagation and seedling growth of magnolia fadou tissue

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Embodiment 1

[0038] A method for fast-propagating seedlings of Fadou magnolia tissue, comprising the following steps:

[0039] (1) Collection of explants: The seeds were collected from Daweishan National Nature Reserve, Pingbian County, Yunnan Province, at an altitude of about 1700m, with a tree age of about 70 years and a tree height of about 30m.

[0040] (2) Disinfection of the surface of the explants: scrub the seeds of Magnolia fadou on the ultra-clean workbench with sterile water, then disinfect them with 75% alcohol by volume, soak for 20 seconds, pour off the alcohol, and then remove the aseptic Rinse with water for 1-2 times, then add 0.1% mercuric chloride solution, soak for 3 minutes, shake continuously during this period, pour out the mercuric chloride solution, rinse 5-6 times with sterile water, and obtain sterile seeds. Blot the water droplets on the surface of the sterile material with sterile filter paper, and put the sterile seeds on the filter paper bridge for cultivatio...

Embodiment 2

[0047] A method for fast-propagating seedlings of Fadou magnolia tissue, comprising the following steps:

[0048] (1) Collection of explants: The seeds were collected from Daweishan National Nature Reserve, Pingbian County, Yunnan Province, at an altitude of about 1700m, with a tree age of about 70 years and a tree height of about 30m.

[0049] (2) Disinfection of the surface of the explants: scrub the seeds of Magnolia fadou on the ultra-clean workbench with sterile water, then disinfect them with 70% alcohol by volume, soak for 30 seconds, pour off the alcohol, and then remove the aseptic Rinse with water for 1-2 times, then add 0.08% mercuric chloride solution by mass fraction, soak for 8 minutes, shake continuously during this period, pour out the mercuric chloride solution, rinse 5-6 times with sterile water, and obtain sterile seeds. Blot the water droplets on the surface of the sterile material with sterile filter paper, and put the sterile seeds on the filter paper bri...

Embodiment 3

[0056] A method for fast-propagating seedlings of Fadou magnolia tissue, comprising the following steps:

[0057] (1) Collection of explants: The seeds were collected from Daweishan National Nature Reserve, Pingbian County, Yunnan Province, at an altitude of about 1700m, with a tree age of about 70 years and a tree height of about 30m.

[0058] (2) Disinfection of the surface of the explants: scrub the seeds of Magnolia fadou on the ultra-clean workbench with sterile water, then disinfect them with 75% alcohol by volume fraction, soak for 30 seconds, pour off the alcohol, and remove the aseptic Rinse with water for 1-2 times, then add 0.05% mercuric chloride solution, soak for 10 minutes, shake constantly during this period, pour out the mercuric chloride solution, rinse 5-6 times with sterile water to obtain sterile seeds. Blot the water droplets on the surface of the sterile material with sterile filter paper, and put the sterile seeds on the filter paper bridge for cultivat...

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Abstract

The invention discloses a tissue rapid propagation seedling cultivation method for manglietia fadouensis, and belongs to the technical field of plant tissue culture. An ML culture medium is set, different hormone components and concentration levels proportion are optimized, seeds are used as explants, complete plants are formed through axillary bud induction, axillary bud proliferation and rooting culture, and after the plants are transplanted to a matrix, robust nursery stock which grows neatly and uniform in appearance is obtained. Axillary bud induction is high in speed, and the inductivity is high; the proliferation coefficient is large, clustered buds are thick and strong, and elongation growth is high in speed; the rooting rate is large, root systems are developed well, rooted seedlings are thick and strong, and the transplant survival rate is large. An effectively path is provided for tissue culture seedling culture of manglietia fadouensis, manglietia fadouensis nursery stock seedlings can be obtained on a large scale through large-scale seedling culture, technical support will be provided for asexual popularization of manglietia fadouensis and even manglietia fordiana plants, and the tissue rapid propagation seedling cultivation method has wide application prospects and is of great significance in landscaping and ecological civilization construction in Guangdong and even the South China.

Description

technical field [0001] The invention belongs to the technical field of tissue culture of plants, and in particular relates to a method for rapid propagation and seedling growth of magnolia fadou tissue. Background technique [0002] Manglietia fadouensis Law et R.Z.Zhou ined belongs to Magnoliaceae (Magnoliaceae) Manglietia (Manglietia), evergreen tree, up to 15m, DBH up to 30cm; bark taupe, smooth; young branches green, old branches Purple-brown, with brown punctate lenticels. The leaves are thin leathery, narrowly ovate-elliptic or narrowly elliptic, 6-12cm long and 2.5-4cm wide. The flower buds are green, oval-shaped; the flowers are white, fragrant and beautiful, with many pistils and white, and many stamens and green, and the flowering period is April to May; the aggregated fruit is bright red when ripe, and the fruit ripening period is September to October. [0003] Fadou magnolia is a masculine tree species, and most of them are forest upper-story trees. Native to ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 邓小梅崔媛媛奚如春赵帅鲁好君叶小玲
Owner SOUTH CHINA AGRI UNIV
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