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Enrichment culture method for nitrifying bacteria

A technology of nitrifying bacteria and culture methods, applied in the direction of bacteria, etc., can solve the problems of increasing sludge production, and achieve the effects of accelerating cell growth, improving sedimentation and stability, and improving enzyme activity

Active Publication Date: 2016-06-01
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, since the adsorbents are mainly zeolite powder, diatomaceous earth, powdered activated carbon or fly ash, the addition of these adsorbents will inevitably increase the sludge production.

Method used

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  • Enrichment culture method for nitrifying bacteria
  • Enrichment culture method for nitrifying bacteria

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] The preparation of embodiment 1 growth promoter

[0018] The preparation method of the nitrifying bacteria growth accelerator of the present invention is: (1) prepare the metal salt solution according to the following composition and parts by weight: the metal salt is 40-100 parts by weight, preferably 50-80 parts by weight, and the metal salt It is calcium salt, ferrous salt and copper salt, Ca in metal salt 2+ , Fe 2+ and Cu 2+ The molar ratio is (5~15):(1~8):(0.5~5), preferably (8~12):(2~6):(1~4); (2) mix 5~ 30 parts by weight, preferably 10-20 parts by weight of polyamines are added to the metal salt solution. Furthermore, before use, the inorganic acid hydroxylamine can also be added in an amount of 0.5-15 parts by weight, preferably 2-10 parts by weight.

[0019] Four types of nitrifying bacteria growth accelerators were prepared according to the ratio and formula of the accelerators in Table 1 by the above-mentioned method, and the concentrations of the accel...

Embodiment 2

[0022] The enrichment culture of embodiment 2 nitrifying bacteria

[0023] Firstly, the activated sludge rich in nitrifying bacteria from a sewage treatment plant was inoculated into five parallel nitrifying bacteria culture reactors (the reactors were respectively named No. 1, No. 2, No. 3, No. 4 and No. 5). After inoculation, the MLSS 3000mg / L, adopt the method of gradually increasing the concentration of substrate ammonia nitrogen for cultivation, the initial concentration of ammonia nitrogen is 50mg / L, increase 50mg / L each time, when the concentration of ammonia nitrogen increases to 300mg / L, it starts to add to reactors 1~4 The growth promoter prepared in Example 1 was added, and No. 5 no promoter was used as a control. See Table 2 for the culture conditions of nitrifying bacteria. When the concentration of ammonia nitrogen in the matrix increased to 500mg / L and the removal rate of ammonia nitrogen in the matrix reached 95% within 24 hours, the enrichment culture process ...

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Abstract

The invention discloses an enrichment culture method for nitrifying bacteria. Active sludge with rich nitrifying bacteria is selected, and a method for gradually increasing ammonia-nitrogen concentration in a substrate is adopted for culturing; the initial ammonia-nitrogen concentration is less than 100mg / L; when the ammonia-nitrogen concentration is increased to 300mg / L, a growth promoter is supplemented; the growth promoter comprises metal salt and a polyamine substance in parts by weight: including 40-100 parts of metal salt, preferably 50-80 parts, and 5-30 parts of polyamine substance, preferably 10-20 parts; the metal salt comprises calcium salt, ferrous salt and copper salt; the molar ratio of Ca<2+> to Fe<2+> to Cu<2+> is (5-15) to (1-8) to (0.5-5), preferably (8-12) to (2-6) to (1-4); and when the ammonia-nitrogen concentration of the substrate is increased to 500mg / L, and the ammonia-nitrogen removal rate within 24h reaches 95%, the culturing is stopped. According to the enrichment culture method, the growth of the nitrifying bacteria is accelerated by adding the growth promoter; the adopted promoter is simple in formula and easy to prepare; the nitrifying bacteria can rapidly grow and propagate after the growth promoter is added; and in addition, the cultured nitrifying bacteria are high in activity and strong in impact resistance.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for enriching and culturing nitrifying bacteria. Background technique [0002] In the biological nitrogen removal method, whether it is the traditional nitrification-denitrification, or the new short-range nitrification-denitrification and short-range nitrification-ANAMMOX, the ammonia nitrogen needs to be removed through the nitrification of nitrifying bacteria. Nitrifying bacteria are chemotrophic microorganisms. Biological cells can only use the energy stored in the form of ATP, etc., and cannot directly use the free energy released by chemical reactions. In aerobic metabolism, ATP is mainly synthesized by oxidative phosphorylation of the respiratory chain. The ammonia oxidative phosphorylation efficiency is very low, and the ATP that can be produced is very limited, and the energy is mainly used for electron transition to a higher energy level, which makes ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20
Inventor 高会杰孙丹凤郭志华赵胜楠
Owner CHINA PETROLEUM & CHEM CORP