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Salinivibrio costicola strain

A salt-producing and vibrio-producing technology, applied in the field of microorganisms, can solve the problems of fish and human cells without the ability to distinguish, and it is difficult to cure and kill shield ciliates, and achieve the effect of stable insecticidal effect.

Active Publication Date: 2016-06-01
THE FIRST INST OF OCEANOGRAPHY SOA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to prevent and control the harm of shield ciliate disease to my country's seawater fish farming industry, many domestic units have screened the control drugs for shield ciliate, but the treatment of the disease in aquatic products is still concentrated on formalin and copper sulfate. 1. The use of zinc sulfate. These drugs basically act on protein denaturation. Although they can kill shield ciliates in water, it is difficult to completely kill shield ciliates in fish, and these drugs have no ability to distinguish between fish and human cells. , can also play a role and cause serious consequences. In addition, Chinese patent documents CN1762460A, CN103349747A have announced two kinds of Chinese herbal medicine formulas for the prevention and treatment of flounder scutellum ciliate disease. Shandong Mariculture Institute once reported that oral trimethoprim ( TMP) against Scutellaria ciliates infected in turbot

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1Y

[0012] Example 1 Isolation and identification of YCSC6 strain

[0013] Step 1. Strain separation culture: The original sample collection location of the strain YCSC6 obtained in the present invention is Daqiao Village, Jimo City, Qingdao (E120.82°, N36.50°). After the sample is collected, it is immediately aseptically coated on MA2216 medium (Difco, article number: 212185), invert the coated petri dish and incubate at 25°C for 24-96 hours, pick the single colony that grows, and streak it on the MA2216 medium plate. Place the petri dish upside down at 25℃ and incubate for 24-96 hours, pick out the single colonies that grow out, and streak it on the MA2216 medium plate again. Put the drawn petri dish upside down at 25℃ and incubate for 24-96 hours After a single colony grows, pick a single colony into 1ml of seawater 2216E liquid medium, and seal it with a parafilm for later use. Place the seawater 2216E liquid medium inoculated with a single colony and place it at 25°C for 24-96 h...

Embodiment 2Y

[0019] Example 2 Monitoring of the killing activity of YCSC6 strain against ciliates

[0020] Step 1. Preparation of YCSC6 bacterial solution: take the spare YCSC6 master plate, streak it on the MA2216 medium plate, place the streaked petri dish upside down at 25°C and incubate for 24-96 hours, pick out the grown single The colonies are streaked and purified on the MA2216 medium plate again, and the streaked petri dish is placed upside down at 25°C for 24-96 hours. When a single colony grows, pick a single colony into 1ml of seawater 2216E liquid medium , And seal it with a parafilm for later use. Inoculate a single colony of seawater 2216E liquid medium and place it at 25°C and shake the bacteria for 24-96 hours until it reaches 0D 600 When it reaches 0.4-0.7, seal it with parafilm for later use.

[0021] Step 2. Preparation of shield ciliate liquid: take the shield ciliate liquid stored in the laboratory, dilute it by 10 times, pick a droplet containing 1 ciliate and immediately ...

Embodiment 3

[0025] Example 3 Insecticidal effect of strain YCSC6 culture supernatant

[0026] Step 1. Strain culture: Pick a single colony of YCSC6 from the spare plate and place it in 1 ml of seawater 2216E liquid medium and place it at 25°C for 36-96 hours until it reaches 0D 600 When it reaches 0.4-0.7, transfer it to 100ml of seawater 2216E liquid medium and place it in 25℃ shaking culture for 36-96 hours, to 0D 600 When it reaches 0.4-0.7, centrifuge at 5000rpm for 10 minutes. After centrifugation, transfer the supernatant to a new sterile tube and seal it with parafilm for later use.

[0027] Step 2. Preparation of shield ciliate liquid: take the shield ciliate liquid stored in the laboratory, dilute it by 10 times, pick a droplet containing 1 ciliate and immediately transfer it to 1ml fish broth medium and place it at 14-18°C for cultivation. When the density of scutellaria in the fish soup medium reaches 5×10 3 When the number / ml is above, transfer the 1ml fish broth medium to 50ml fish...

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PUM

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Abstract

The invention provides a Salinivibrio costicola YCSC6 strain of which the collection number is CGMCC No.11730. The Salinivibrio costicola strain is used for preparing products for preventing and treating Paralembus digitiformis. When being cocultured with salt water fish Paralembus digitiformis pathogens, the Salinivibrio costicola can kill more than 98% of Paralembus digitiformis in the coculture system within 12 hours. After continuous multiple-generation culture, the Salinivibrio costicola strain has stable insecticidal effect effects. The detection of the strain provides a new biocontrol strain for preventing and treating salt water fish Paralembus digitiformis.

Description

Technical field [0001] The invention belongs to the technical field of microorganisms, and specifically relates to a kind of Halobacterium costum. Background technique [0002] Shield ciliates are the most common and the most harmful disease in the late stage of sea flounder fish nursery. It mainly occurs in juveniles with a total length of 2-15 cm after the juveniles bottom. Reports show that Shield ciliates can occur from 12 to 26 ℃ in factory farming workshops in various parts of my country, and the disease can occur almost throughout the year. Diseases in the marine aquaculture industry have always been one of the important factors affecting the success or failure of aquatic products. The occurrence of diseases caused by ciliated protozoa has become more frequent in recent years and has attracted people's attention. Scutellaria is a kind of facultative parasitic ciliates, which can live freely in water bodies. They feed on bacteria and organic debris. After invading cultured...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01P15/00C12R1/01
CPCA01N63/10C12N1/205C12R2001/01
Inventor 曲凌云田欣欣杜光讯
Owner THE FIRST INST OF OCEANOGRAPHY SOA