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Modification method of collagen

A collagen, modification and modification technology is applied in the field of modification and modification of collagen to achieve the effect of improving gel strength and overcoming poor mechanical properties

Active Publication Date: 2016-06-01
湖南生命元特医生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, chemical cross-linking agents such as genipin and glutaraldehyde have strong cytotoxicity, and a high concentration of Mg was introduced during the neutral salt modification process. 2+ ions, so it is limited to use in the tanning industry, but not in the fields of food and medicine

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] The present embodiment provides a modification modification method of collagen, it comprises the following steps:

[0020] (a) 50 g of collagen (commercially available) was dissolved in 0.5 mol / L, 500 ml of acetic acid solution, heated and stirred at 40° C. to dissolve, and the undissolved part was filtered to form a collagen saturated solution;

[0021] (b) Take 100 g (89.5 ml) of the above-mentioned saturated collagen solution, add 0.01 g of proteolytic enzyme therein, and heat and react at 40° C. for 3 hours;

[0022] (c) add 8.95ml chitosan acetic acid solution (concentration is 2wt%) and catalyzer 10g in the product of step (b), get final product at 40 ℃ heating reaction 2 hours; Described catalyzer is made of 0.2gSm 2 o 3 , 0.3gEu 2 o 3 and 0.5gGd 2 o 3 composition.

Embodiment 2

[0024] The present embodiment provides a modification modification method of collagen, it comprises the following steps:

[0025] (a) Dissolve 50g of collagen (extracted from shad skin by existing technology) in 1.5mol / L, 500ml of acetic acid solution, heat and stir at 50°C to dissolve, and filter to remove the undissolved part to form a collagen saturated solution ;

[0026] (b) Take 400g (358ml) of the above-mentioned saturated collagen solution, add 0.4g proteolytic enzyme therein, and heat and react at 50°C for 1 hour;

[0027] (c) in the product of step (b), add 71.6ml chitosan acetic acid solution (concentration is 2wt%) and catalyzer 4g, get final product in 50 ℃ of heating reactions 0.5 hour; Described catalyzer is made of 0.6gSm 2 o 3 , 1gEu 2 o 3 and 2.4gGd 2 o 3 composition.

Embodiment 3

[0029] The present embodiment provides a modification modification method of collagen, it comprises the following steps:

[0030] (a) Dissolve 50 g of collagen (extracted to the front elbow of pig’s trotter by using the prior art) in 2 mol / L, 500 ml of acetic acid solution, heat and stir at 50° C. to dissolve, and filter to remove the undissolved part to form a collagen saturated solution;

[0031] (b) Take 400g (358ml) of the above collagen saturated solution, add 0.1g proteolytic enzyme therein, and heat and react at 45°C for 1.5 hours;

[0032] (c) add 75ml chitosan acetic acid solution (concentration is 2wt%) and catalyzer 1g in the product of step (b), get final product at 45 ℃ of heating reaction 2 hours; Described catalyzer is made of 0.25gSm 2 o 3 , 0.3gEu 2 o 3 and 0.45gGd 2 o 3 composition.

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Abstract

The invention relates to a modification method of collagen.The modification method comprises the following steps that 1, the collagen is dissolved into an acetate solution to prepare a saturated collagen solution; 2, a proteolytic enzyme is added into the saturated collagen solution, and reacting under heating is performed for 1-3 hours at the temperature of 40 DEG C-50 DEG C; 3, a chitosan acetic acid solution and a catalyst are added into a product generated in the step 2, and reacting under heating is performed for 0.5-2 hours at the temperature of 40 DEG C-50 DEG C, wherein the catalyst is prepared from, by mass, 15%-25% of Sm2O3, 25%-35% Eu2O3 and 45%-60% Gd2O3.By adding the chitosan acetic acid solution and the catalyst into the saturated collagen solution generated through reacting of the proteolytic enzyme, the collagen reacts with the chitosan acetic acid to generate a crosslinking substance, therefore, the jelly strength of the collagen is improved, the defect that the mechanical property of the collagen is poor is overcome, and the collagen can be applied to the food and medicine fields.

Description

technical field [0001] The invention belongs to the field of protein processing, and in particular relates to a method for modifying collagen. Background technique [0002] Collagen is the most abundant protein in animals. Due to its special structure and function, collagen has been widely used in medicine, food and cosmetics and other fields. At the same time, collagen is also the main supporting structure of human tissue, the main structural protein of the human body, and constitutes about 30% of the protein in the human body. Collagen has the characteristics of low antigenicity, high affinity with cells, can promote cell growth, and coagulate with platelets, and because of its excellent biocompatibility, collagen can be called the most suitable tissue engineering material, but natural collagen The manufactured tissue engineering products and medical implants such as sutures have low mechanical strength, poor mechanical properties, and too fast degradation rate, which can...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/113
CPCC07K14/78C12P21/06
Inventor 邵晨
Owner 湖南生命元特医生物科技有限公司
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