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Preparation method for extracting agar under assistance of compound enzyme method

A technology of auxiliary extraction and compound enzyme method, applied in the direction of fermentation, etc., can solve the problems of environmental pollution, high concentration of alkali treatment, etc., achieve the effect of reducing production cost, reducing the amount of acid, and improving competitiveness

Inactive Publication Date: 2015-09-16
JIMEI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is to provide a kind of preparation method of compound enzymatic auxiliary extraction agar, to solve the alkali treatment concentration that traditional alkali modification technology exists, serious environmental pollution defect, meanwhile, according to this preparation method, the prepared Agar has the characteristics of viscosity, transparency, gel strength and high extraction rate

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] (1) Alkali treatment: Weigh 25 kg of dried Gracilaria without impurity removal treatment, add the prepared sodium hydroxide solution with a concentration of 7%, the ratio of solid to liquid is 1:30, and the alkali treatment temperature is 90°C. The time is 3 hours.

[0024] (2) Alkali washing: Alkali washing was divided into five times, the first two times were washed for 30 minutes each, and the last three times were washed for 40 minutes each. After alkali treatment, Gracilaria became neutral after five times of washing.

[0025] (3) Acidification: For the first acidification, the mass ratio of Gracilaria to sulfuric acid is 50:1, and the acidification time is 40 minutes. After acidification, water is added for cleaning for 10 minutes. In the second acidification, the mass ratios of Gracilaria to sulfuric acid, oxalic acid, and EDTA were 50:1, 55:1, and 280:1, respectively. Wash twice after the second acidification, the first time for 10 minutes, and the second time ...

Embodiment 2

[0031] (1) Enzyme treatment: Weigh 25 kg of dried Gracilaria without impurity removal treatment, add water 30 times the weight of the algae and soak for 30 minutes, after controlling the water, add the same amount of water, and add the same amount of water as the algae to the system Cellulase and sulfatase with a body weight ratio of 0.01%, the temperature is controlled at 45-50°C, the enzymatic hydrolysis time is 1 hour, and the water is controlled after the enzymatic hydrolysis.

[0032] (2) Alkali treatment: Add the prepared sodium hydroxide solution with a concentration of 3%, the ratio of solid to liquid is 1:30, the alkali treatment temperature is 90°C, and the alkali treatment time is 4 h.

[0033] (3) Alkali washing: Alkali washing was divided into five times, the first two times were washed for 30 minutes each, and the last three times were washed for 40 minutes each. After alkali treatment, Gracilaria became neutral after five times of washing.

[0034] (4) Acidifica...

Embodiment 3

[0040] (1) Enzyme treatment: Weigh 25 kg of dried Gracilaria without impurity removal treatment, add water 30 times the weight of the algae and soak for 30 minutes, after controlling the water, add the same amount of water, and add a mass ratio of 0.01% cellulase and sulfatase, the temperature is controlled at 45-50°C, the enzymatic hydrolysis time is 1 h, and the water is controlled after the enzymatic hydrolysis.

[0041] (2) Alkali treatment: Add the prepared sodium hydroxide solution with a concentration of 3%, the ratio of solid to liquid is 1:30, the alkali treatment temperature is 90°C, and the alkali treatment time is 4 h.

[0042] (3) Alkali washing: Alkali washing was divided into five times, the first two times were washed for 30 minutes each, and the last three times were washed for 40 minutes each. After alkali treatment, Gracilaria became neutral after five times of washing.

[0043] (4) Acidification: The mass ratios of Gracilaria to sulfuric acid, oxalic acid, ...

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Abstract

The invention discloses a preparation method for extracting agar under the assistance of a compound enzyme method. The preparation method comprises the steps of enzyme treatment, alkali treatment, alkali washing, acidification, acid washing, bleaching, decolorizer washing, agar extraction and fine filtration. The preparation method is environmentally friendly. Compared with a traditional method, by the adoption of the compound enzyme method, the concentration of alkali is reduced, the use amount of acid is reduced, and thus discharge of sewage is reduced; meanwhile, by the adoption of the compound enzyme method, the agar extracting rate is increased, the transparency, the viscosity and the strength of agar are improved, and thus the preparation method has broad application prospects.

Description

technical field [0001] The invention relates to the technical field of agar, in particular to a method for preparing agar by compound enzyme method assisted extraction of agar. Background technique [0002] As an important raw material for extracting agar, Gracilaria is rich in a large amount of colloid, and its content accounts for 20% to 30% of the dry weight. Agar gel, also known as agar, has the functions of detoxification and beautification, lowering blood pressure, lowering blood sugar, and improving immunity. It is widely used in many fields such as food, medicine, daily chemical industry, and bioengineering. The slow development of my country's agar industry is mainly due to the limitation of raw materials. The annual output of agar in the world is only about 20,000 tons. Therefore, the price of agar in the international market continues to rise. At present, the process of producing agar in most domestic enterprises generally includes several steps such as alkali tr...

Claims

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Application Information

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IPC IPC(8): C12P19/04C08B37/12
Inventor 肖安风倪辉蔡慧农肖琼杜希萍朱艳冰吴昌正杨秋明陈艳红
Owner JIMEI UNIV
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