Preparation method for extracting agar under assistance of compound enzyme method

A technology of auxiliary extraction and compound enzyme method, applied in the direction of fermentation, etc., can solve the problems of environmental pollution, high concentration of alkali treatment, etc., achieve the effect of reducing production cost, reducing the amount of acid, and improving competitiveness

Inactive Publication Date: 2015-09-16
JIMEI UNIV
View PDF4 Cites 20 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is to provide a kind of preparation method of compound enzymatic auxiliary extraction agar, to solve the alkali treatment concentration that traditional alkali modification

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0022] Example 1

[0023] (1) Alkali treatment: Weigh 25 kg of dry Gracilaria without impurity removal treatment, add the prepared sodium hydroxide solution with a concentration of 7%, the ratio of material to liquid is 1:30, the alkali treatment temperature is 90℃, and the alkali treatment The time is 3 hours.

[0024] (2) Alkali washing: Alkali washing is divided into five times, the first two washings are 30 minutes each, and the last three washings are 40 minutes each. After the alkali treatment, Gracilaria can be neutralized after five washings.

[0025] (3) Acidification: For the first acidification, the mass ratio of Gracilaria to sulfuric acid is 50:1, and the acidification time is 40min. After acidification, water is added for cleaning for 10 minutes. In the second acidification, the mass ratios of Gracilaria, sulfuric acid, oxalic acid, and EDTA were 50:1, 55:1, and 280:1, respectively. Washing twice after the second acidification, the first washing for 10 minutes, and th...

Example Embodiment

[0030] Example 2

[0031] (1) Enzyme treatment: Weigh 25 kg of dry Gracilaria without impurity removal treatment, add water 30 times the weight of the algae and soak for 30 min. After controlling the water, add the same amount of water and add the same amount of water to the system. Cellulase and sulfatase with a body weight ratio of 0.01%, temperature control 45-50 ℃, enzymatic hydrolysis time 1 h, after enzymatic hydrolysis, control the net water.

[0032] (2) Alkali treatment: add a prepared 3% sodium hydroxide solution, the ratio of material to liquid is 1:30, the alkali treatment temperature is 90℃, and the alkali treatment time is 4 hours.

[0033] (3) Alkali washing: Alkali washing is divided into five times, the first two washings are 30 minutes each, and the last three washings are 40 minutes each. After alkaline treatment, Gracilaria serrata becomes neutral after five washings.

[0034] (4) Acidification: For the first acidification, the mass ratio of Gracilaria to sulfuric...

Example Embodiment

[0039] Example 3

[0040] (1) Enzyme treatment: Weigh 25 kg of dry Gracilaria without impurity removal treatment, add 30 times the weight of the algae to soak for 30 minutes, control the water, add the same amount of water, and add the mass ratio to the system It is 0.01% cellulase and sulfatase, the temperature is controlled at 45~50℃, the enzymolysis time is 1 h, and the water is controlled after enzymolysis.

[0041] (2) Alkali treatment: add a prepared 3% sodium hydroxide solution, the ratio of material to liquid is 1:30, the alkali treatment temperature is 90℃, and the alkali treatment time is 4 hours.

[0042] (3) Alkali washing: Alkali washing is divided into five times, the first two washings are 30 minutes each, and the last three washings are 40 minutes each. After alkaline treatment, Gracilaria serrata becomes neutral after five washings.

[0043] (4) Acidification: The mass ratios of Gracilaria, sulfuric acid, oxalic acid and EDTA in the acidification process are 93:1, 5...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Viscosityaaaaaaaaaa
Gel strengthaaaaaaaaaa
Viscosityaaaaaaaaaa
Login to view more

Abstract

The invention discloses a preparation method for extracting agar under the assistance of a compound enzyme method. The preparation method comprises the steps of enzyme treatment, alkali treatment, alkali washing, acidification, acid washing, bleaching, decolorizer washing, agar extraction and fine filtration. The preparation method is environmentally friendly. Compared with a traditional method, by the adoption of the compound enzyme method, the concentration of alkali is reduced, the use amount of acid is reduced, and thus discharge of sewage is reduced; meanwhile, by the adoption of the compound enzyme method, the agar extracting rate is increased, the transparency, the viscosity and the strength of agar are improved, and thus the preparation method has broad application prospects.

Description

technical field [0001] The invention relates to the technical field of agar, in particular to a method for preparing agar by compound enzyme method assisted extraction of agar. Background technique [0002] As an important raw material for extracting agar, Gracilaria is rich in a large amount of colloid, and its content accounts for 20% to 30% of the dry weight. Agar gel, also known as agar, has the functions of detoxification and beautification, lowering blood pressure, lowering blood sugar, and improving immunity. It is widely used in many fields such as food, medicine, daily chemical industry, and bioengineering. The slow development of my country's agar industry is mainly due to the limitation of raw materials. The annual output of agar in the world is only about 20,000 tons. Therefore, the price of agar in the international market continues to rise. At present, the process of producing agar in most domestic enterprises generally includes several steps such as alkali tr...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12P19/04C08B37/12
Inventor 肖安风倪辉蔡慧农肖琼杜希萍朱艳冰吴昌正杨秋明陈艳红
Owner JIMEI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap