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Method for preparing high-quality biological polysaccharide

A technology of mass percentage content and xanthan gum, which is applied in the field of purification of xanthan gum coarse powder, can solve the problems of destroying product properties, reducing product recovery rate, etc., achieving low cost, fewer production process steps, and simple and easy technical operation The effect of mastering

Active Publication Date: 2016-06-08
ZHEJIANG GLLION BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, through the above methods, the product recovery rate may be reduced, or the product properties may be destroyed to a certain extent, for example, the reagents used in the chemical deproteinization process may react with xanthan gum

Method used

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  • Method for preparing high-quality biological polysaccharide
  • Method for preparing high-quality biological polysaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050]1. Preparation of xanthan collagen sample solution: Mix Fufeng food grade 80 mesh xanthan gum powder with 10 times the weight of glucose powder evenly, add a small amount of high-speed stirring distilled water at 90°C one by one, continue stirring until fully dissolved, and prepare the quality A xanthan collagen-like solution with a percentage content of 0.25%, and cooled to about 37° C. for use.

[0051] 2. Lipase enzymatically removes impurities: dissolve Solarbio lipase (L8070, enzyme activity 100-400U / mg) in physiological phosphate buffer, add the lipase solution to the 0.25 % xanthan collagen-like solution. Afterwards, the mixture was stirred and mixed at 37°C for 30 minutes, and left at 37°C for 12 hours for enzymatic hydrolysis.

[0052] 3. Enzyme inactivation: heating the xanthan gum solution after the enzymolysis treatment in step 2 in a boiling water bath to inactivate lipase.

[0053] 4. Vacuum suction filtration: according to 311# (clarifying filter cardboa...

Embodiment 2

[0061] 1. Preparation of xanthan collagen sample solution: add Fufeng food grade 200-mesh xanthan gum powder into distilled water at 90°C with high-speed stirring in small amounts until fully dissolved, and prepare a xanthan collagen sample with a mass percentage of 0.25%. solution and cooled to about 40°C for later use.

[0062] 2. Lipase enzymatically removes impurities: dissolve Solarbio lipase (L8070, enzyme activity 100-400U / mg) in physiological phosphate buffer, add the lipase solution to the 0.25 % xanthan collagen-like solution. Afterwards, the mixture was continuously stirred at 40° C. for 2 h to carry out enzymatic hydrolysis.

[0063] 3. Enzyme inactivation: heat the xanthan gum solution after enzymolysis in step 2 in a water bath at 90°C to inactivate the enzyme.

[0064] 4. Vacuum suction filtration: in the order of 311# (pore size 10 μm) → 321# (pore size 1.5 μm) → 332# (pore size 0.8 μm) → 334# (pore size 0.45 μm), use the filter plate to inactivate the enzyme...

Embodiment 3

[0071] 1. Preparation of xanthan collagen sample solution: add Fufeng food grade 200 mesh xanthan gum powder into distilled water and stir evenly, and heat to 80°C to fully dissolve it, and prepare xanthan collagen with a mass percentage of 0.50% The sample solution was cooled to about 40°C for later use.

[0072] 2. Activated carbon adsorption and impurity removal: Add activated carbon with a volume percentage of about 2% to the 0.50% xanthan collagen-like solution prepared in step 1, stir well, and absorb at 4°C overnight, use 300-mesh filter cloth and 311# (pore size 10 μm) filter plate vacuum filtration to remove activated carbon.

[0073] 3. Lysozyme enzyme decontamination: Dissolve Solarbio lysozyme (L8020, enzyme activity >20000U / mg) in physiological phosphate buffer, add lysozyme solution according to the enzyme dosage of 5000U / ml, and remove it by activated carbon adsorption in step 2 in the mixed xanthan gum solution. Afterwards, the mixture was continuously stirre...

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Abstract

The invention discloses a method for preparing high-transparency xanthan gum by purifying ordinary xanthan gum. The method comprises the following steps: enzyme solutions including a lipase solution and the like are added into a xanthan gum original solution, and stirred and uniformly mixed for enzymolysis, so that impurities in xanthan gum originals are removed, and a xanthan gum solution after enzymolysis and impurity removal is obtained; a clarification filtration paper board, a fine filtration paper board and a partial bacterium removal filtration paper board are adopted to perform depressurization and suction filtration on the xanthan gum solution after enzymolysis and impurity removal; the xanthan gum solution after suction filtration is mixed with an alkoxide mixed solution, the mixture is stirred till xanthan gum precipitates out, the xanthan gum is rinsed with ethyl alcohol, immersed overnight and dried, and a high-transparency xanthan gum product is obtained. The method has the advantages that the production technological process for the high-transparency xanthan gum has few steps, the technology operation is simple and easy to master, the cost is low, and the transparency of the product is high.

Description

technical field [0001] The invention relates to a method for purifying coarse xanthan gum powder, in particular to a method for purifying common xanthan gum to prepare highly transparent xanthan gum. Background technique [0002] Xanthan gum (Xanthangum) is a natural polysaccharide produced by the fermentation of the bacteria Xanthomonascampetris. It has the characteristics of low toxicity to the human body, indigestibility, high viscosity and excellent stability. It is widely used in food, petroleum industry, medicine, cosmetics, personal Nursing products and agricultural production. [0003] Xanthan gum is very soluble in water, and the rubber powder is easy to agglomerate during the dissolution process. Its special molecular structure makes its aqueous solution have non-Newtonian rheology (pseudoplastic characteristics), that is, shear thinning, followed by rapid recovery of viscosity. In addition, different temperatures lead to changes in the conformation of colloidal ...

Claims

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Application Information

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IPC IPC(8): C08B37/02
CPCC08B37/0033
Inventor 郭宏亮庄秀园童晓梅武自强林檬王轩
Owner ZHEJIANG GLLION BIOTECH CO LTD
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