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Method for determining toxicity of anabasine pesticide by using daphnia magna

A technology of neonicotinoids and large fleas, applied in the field of aquaculture, can solve the problems of less research and lack of research data to evaluate the practicability and reliability of the model, and achieve the effects of high accuracy, strong practicability and easy operation.

Inactive Publication Date: 2016-06-08
HUNAN PLANT PROTECTION INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, daphnia magna has been widely used in the research of pesticide environmental safety assessment, but there are few studies using daphnia magna as a neonicotinoid pesticide toxicity screening model, and there is a lack of relevant research data to evaluate the practicability and reliability of the model

Method used

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  • Method for determining toxicity of anabasine pesticide by using daphnia magna
  • Method for determining toxicity of anabasine pesticide by using daphnia magna
  • Method for determining toxicity of anabasine pesticide by using daphnia magna

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] (1) Preparation of pesticide stock solution:

[0027] Weigh 0.5000g of 5% acetamiprid EC, and use ElendtM 4 The culture solution was dissolved and adjusted to a 100mL volumetric flask to prepare a stock solution with a concentration of 250mga.i. / L.

[0028] (2) Preparation of test solution:

[0029] Elendt M 4 Preparation of culture medium: ElendtM 4 The culture solution was aerated by the air aeration pump for more than 24 hours, and the ElendtM 4 The pH of the culture solution was 7.11, the hardness was 192 mg / L, and the dissolved oxygen was 7.56 mg / L.

[0030] Experimental group: Pipette 0.300mL, 0.600mL, 1.200mL, 2.400mL, 4.800mL, 9.60mL of the stock solution in turn, respectively use ElendtM 4 Dilute the culture solution to a 250mL volumetric flask to obtain a concentration of 0.300mga.i. / L, 0.600mga.i. / L, 1.20mga.i. / L, 2.40mga.i. / L, 4.80mga.i. / L, 9.60mga.i. / L test solution. Use a 100mL beaker to fill 50mL of the corresponding test solution.

[0031] Blank...

Embodiment 2

[0050] (1) Preparation of pesticide stock solution:

[0051] Weigh 1.1905g of 21% thiamethoxam suspension, use ElendtM 4 Dissolve the culture solution and dilute it to a 100mL volumetric flask, and prepare a concentration of 2.50×10 3 mga.i. / L pesticide stock solution.

[0052] (2) Preparation of test solution:

[0053] Elendt M 4 Preparation of culture medium: ElendtM 4 The culture solution was aerated by the air aeration pump for more than 24 hours, and the ElendtM 4 The pH of the culture solution was 7.13, the hardness was 184 mg / L, and the dissolved oxygen was 7.49 mg / L.

[0054] Experimental group: pipette 0.100mL, 0.180mL, 0.324mL, 0.583mL, 1.050mL of the pesticide stock solution in step (1) in sequence and use ElendtM 4 Dilute the culture solution to a 250mL volumetric flask to obtain a concentration of 1.00mga.i. / L, 1.80mga.i. / L, 3.24mga.i. / L, 5.83mga.i. / L, 10.5mga.i. / L of test liquid. The experimental group set up 4 repetitions.

[0055] Blank control group ...

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Abstract

The invention provides a method for determining the toxicity of an anabasine pesticide by using daphnia magna. The method comprises the following steps: dissolving the anabasine pesticide to prepare a pesticide stock solution; mixing the pesticide stock solution with an Elendt M4 culture solution to prepare a mixed solution with gradient concentrations, continuously culturing for 48h with the mixed solution as domestic water of the daphnia magna, and recording the biological effect parameters of the daphnia magna and the physical and chemical parameters of the mixed solution every 24h in the culture process; and calculating the median inhibitory concentration EC50 of the pesticide to the daphnia magna according to the biological effect parameters of the daphnia magna and the physical and chemical parameters of the pesticide stock solution, and judging the toxicity of the anabasine pesticide. The method has the advantages of simple operation, strong practicality, fast detection speed and high accuracy.

Description

technical field [0001] The invention relates to the field of breeding technology, in particular to a method for determining the toxicity of neonicotinoid pesticides in Daphnia magna. Background technique [0002] Daphnia magna is a crustacean Cladocera invertebrate widely distributed in the world. It feeds on phytoplankton and is also a bait for fish and large invertebrates. It is an important source of material circulation and energy flow in aquatic ecosystems. links. Due to its wide distribution, fast reproduction, short life cycle, easy cultivation and domestication, sensitivity to poisons in the environment and simple experimental methods, it has been valued by toxicologists in many countries. It has become a standard test organism and is widely used in aquatic toxicology research. [0003] At present, Daphnia magna has been widely used in the research of environmental safety assessment of pesticides, but there are few studies using Daphnia magna as a screening model f...

Claims

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Application Information

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IPC IPC(8): G01N33/50
CPCG01N33/5014
Inventor 陈昂刘勇张德咏罗香文严清平刘建宇罗源华
Owner HUNAN PLANT PROTECTION INST
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