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A kind of culture medium, biotransformation mycelium, extract and use
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A technology of biotransformation and mycelium, applied in application, fungi, drug combination, etc., can solve the problem that the mycelium of Hericium erinaceus has no anticoagulant effect, etc.
Active Publication Date: 2019-11-19
HUNAN XINHUI PHARMA
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Problems solved by technology
[0008] According to the literature search, Hericium erinaceus mycelium has no anticoagulant effect
Method used
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Embodiment 1
[0028] A kind of biotransformation mycelia extract, its preparation method comprises the steps:
[0029] (1) Take 490g of wheat bran, 140g of cornmeal, 63g of rice bran, 7g of sugar, and 300g of Chinese herbal medicineGinkgo biloba powder (Ginkgo biloba), mix well, add about 500g of water and mix well to obtain the culture medium. Then bottle, sterilize, insert Hericium erinaceus, transform and cultivate at 27°C for 40 days, take out the mycelium, and dry it to obtain the biotransformed mycelium;
[0030] (2) Take the biotransformed mycelium, soak it in water at 60° C. for 2 times, soak for 2 hours each time, and the weight of the water is 6 times the weight of the biotransformed mycelium;
[0031] (3) Concentrate the extract obtained after extraction under reduced pressure, and vacuum-dry the concentrated solution obtained by concentrating under reduced pressure at 60° C. to obtain the extract.
Embodiment 2
[0033] A kind of biotransformation mycelia extract, its preparation method comprises the steps:
[0034] (1) Take 420g of wheat bran, 120g of cornmeal, 54g of rice bran, 6g of sugar, and 400g of Chinese herbal medicineGinkgo biloba powder (Ginkgo biloba), mix well, add about 500g of water and mix well to obtain mycelium culture medium bottle, sterilize, insert Hericium erinaceus, transform and culture at 20°C for 35 days, take out the mycelium, and dry it to obtain the biotransformed mycelium;
[0035] (2) Take the biotransformation mycelium, soak it in water at 60°C for 3 times, soak for 3 hours each time, and the weight of the water is 8 times the weight of the biotransformation mycelium;
[0036] (3) Concentrate the extract obtained after extraction under reduced pressure, and vacuum-dry the concentrated solution obtained by concentrating under reduced pressure at 60° C. to obtain the extract.
Embodiment 3
[0038] A kind of biotransformation mycelia extract, its preparation method comprises the steps:
[0039] (1) Take 350g of wheat bran, 100g of cornmeal, 45g of rice bran, 5g of sugar, and 500g of Chinese herbal medicineGinkgo bilobapowder (Ginkgo biloba), mix well, add about 500g of water and mix well to obtain mycelium culture medium bottle again, sterilize, insert Hericium erinaceus, transform and cultivate at 25°C for 30 days, take out the mycelium, and dry it to obtain the biotransformed mycelium;
[0040] (2) Take the biotransformed mycelium, soak it in water at 50°C for 3 times, soak for 4 hours each time, and the weight of the water is 10 times of the weight of the biotransformed mycelium;
[0041] (3) Concentrate the extract obtained after extraction under reduced pressure, and vacuum-dry the concentrated solution obtained by concentrating under reduced pressure at 70° C. to obtain the extract.
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Abstract
The invention belongs to the technical field of biological pharmacy, and particularly relates to a culture medium, a biological transformation mycelium, extract and application. The culture medium comprises, by weight, 490-350 parts of wheat bran, 140-100 parts of corn meal, 63-45 parts of bran coat, 7-5 parts of white sugar, 300-500 parts of folium ginkgo and 400-600 parts of water. A method for preparing the biological transformation mycelium includes inoculating hericium erinaceus strains into the culture medium; cultivating hericium erinaceus at the temperature of 20-27 DEG C for 30-40 days; drying the hericium erinaceus to obtain the biological transformation mycelium. A method for preparing the extract includes extracting the biological transformation mycelium by the aid of water to obtain extract liquid; concentrating the extract liquid under reduced pressures; drying the extract liquid under vacuum conditions to obtain the extract. The culture medium, the biological transformation mycelium, the extract and the application have the advantages that the coagulation activity can be obviously improved by the extract, obvious anti-coagulation effects can be realized, and accordingly the culture medium, the biological transformation mycelium and the extract can be applied to preparing anti-coagulation medicines or healthcare foods.
Description
technical field [0001] The invention belongs to the technical field of biopharmaceuticals, and specifically relates to a culture medium, biotransformation mycelium, extract and application. Background technique [0002] Blood coagulation refers to the process in which blood changes from a flowing liquid state to an immobile gel state, and is an important part of the physiological hemostasis process. The essence of blood coagulation is the process of converting soluble fibrinogen in plasma into insoluble fibrin. When the formed fibrin interweaves into a network, blood cells and other blood components can be trapped, thereby forming a blood clot. Blood coagulation is a series of complex enzymatic reaction process, which requires the participation of various coagulation factors. The activation of prothrombin is carried out under the action of prothrombin enzyme complex. Therefore, the coagulation process can be divided into three basic processes: formation of prothrombin enz...
Claims
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Application Information
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