Unlock instant, AI-driven research and patent intelligence for your innovation.

Probe used for detecting and locating swine fever exogenous virus and bovine viral diarrhea virus (BVDV) and kit

A technology for bovine viral diarrhea and viruses, applied in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc., can solve the problems of poor detection sensitivity and low signal group strength, and achieve clear detection and Positioning, clear visualization effect

Inactive Publication Date: 2016-06-15
HUNAN SINOLAND BIOLOGICAL PHARMA
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of this, the purpose of the present invention is to propose a probe and kit for detecting and locating CSF exogenous virus bovine viral diarrhea virus. The present invention has the characteristics of high sensitivity and stability, and overcomes the traditional fluorescence The technical problems of low signal group intensity and poor detection sensitivity in in situ hybridization

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Probe used for detecting and locating swine fever exogenous virus and bovine viral diarrhea virus (BVDV) and kit
  • Probe used for detecting and locating swine fever exogenous virus and bovine viral diarrhea virus (BVDV) and kit
  • Probe used for detecting and locating swine fever exogenous virus and bovine viral diarrhea virus (BVDV) and kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0024] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be described in further detail below in conjunction with specific embodiments and with reference to the accompanying drawings.

[0025] In recent years, QuantiGeneViewRNA technology has been applied in the research of major human diseases such as cancer and AIDS, and the research on the pathogenic mechanism of these diseases has provided reliable evidence, but there are few reports in the detection of veterinary pathogenic microorganisms. The technology is applied in the detection and localization of the exogenous virus of swine fever, bovine viral diarrhea virus. As an embodiment of the present invention, the probe provided by the present invention for detecting and locating bovine viral diarrhea virus includes 4 specific probe sequences for bovine viral diarrhea virus:

[0026] P1: 5'-ATCTCTGCTGTACATGGCACATGGAGTTGATCACAAAT-3';

[0027] P2: 5'-AG...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Transmittanceaaaaaaaaaa
Login to View More

Abstract

The invention discloses a probe and a kit for detecting and locating the bovine viral diarrhea virus, an exogenous virus of swine fever, including 4 specific probe sequences for the bovine viral diarrhea virus: P1: 5'-ATCTCTGCTGTACATGGCACATGGAGTTGATCACAAAT -3'; P2: 5'-AGGTGGGTCCATAATAGGCTTGACTGCCCTCT-3'; P3: 5'-ATTCAAAGAACATGAACGCATTAGTATGTTCCAGGA-3'; and P4: 5'-GCTTCTTGGTACGGGGAGGAGGAAGTCTACGGTATGCCA-3'. The present invention can specifically, sensitively, quickly and easily detect and locate target RNA from cultured cells, tissues and clinical samples, and can clearly and visually detect and locate bovine viral diarrhea virus RNA, thereby being able to detect and locate BVDV at the RNA level The replication and proliferation rules of viral RNA during the infection process are described.

Description

technical field [0001] The invention relates to the field of animal molecular virology, in particular to a probe and a kit for detecting and locating the exogenous virus of swine fever, bovine viral diarrhea virus. Background technique [0002] Bovine viral diarrhea virus (Bovineviraldiarrheavirus, BVDV) is the pathogen of bovine viral diarrhea / mucosaldisease (BVD / MD). Since it was described and identified in the United States by Olafson et al. Developed countries such as New Zealand, Canada, Argentina, Japan, India, Africa and Europe, etc. In the early 1980s, BVDV was first isolated from foreign sheep, dairy cows and frozen semen in China. After BVDV infects cattle, its clinical symptoms can be manifested as mucous membrane disease, diarrhea, abortion, stillbirth and deformed fetus, etc., which have caused great economic losses to the cattle industry. Bulletin No. 96 of the Ministry of Agriculture of my country in 2008 defined bovine viral diarrhea as a three-category dis...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/70C12Q1/68C12N15/11
CPCC12Q1/701C12Q2600/166
Inventor 赵燕王和平喻正军张桂红
Owner HUNAN SINOLAND BIOLOGICAL PHARMA