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Preparation method of meniscal scaffold

A meniscus and bone chip technology, applied in medical science, tissue regeneration, prosthesis, etc., can solve problems such as poor repeatability and stability of measurement data, difference in meniscus fiber shape, and inability to connect directly.

Active Publication Date: 2016-06-29
PEKING UNIV THIRD HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0008] For the meniscus tissue, classic biomechanical experiments cannot accurately describe the biomechanical properties of the meniscus. It has been reported that the biomechanical properties of different positions of the meniscus are different, which may be caused by the difference in the shape of the fibers inside the meniscus.
In our laboratory’s earlier detection of classic biomechanical pull-off and reciprocating pressure on meniscus tissue, it was found that due to the small size of the meniscus tissue, it could not be directly connected to the classic biomechanical testing machine. The fixture is specially designed and manufactured for the data, but the repeatability and stability of the measurement data are poor. Therefore, it is currently the industry's best choice to select a suitable test machine and test parameters, and through the control of a suitable preparation method, to obtain a bracket that meets the normal biomechanical requirements. problem to be solved

Method used

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  • Preparation method of meniscal scaffold
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  • Preparation method of meniscal scaffold

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Select the femur or tibia of the animal, remove the muscle, periosteum and bone marrow, and obtain the tubular long bone part without cancellous bone in the middle part of the femoral shaft and below the tibial plateau. The bone segmentation machine cuts the bone canal into bone slices, and selects relatively flat bone slices as the material for tissue engineering menisci;

[0044] Use 1mol / L buffered hydrochloric acid decalcification solution for decalcification, and use a pH meter to detect that the pH value of the decalcification solution is greater than 1.5 and replace the decalcification solution. The bone flakes are completely in contact with the decalcification solution;

[0045] After replacing the decalcification solution, measure the pH value of the decalcification solution for 5 consecutive days. If the difference between the pH value of the decalcification solution and the initial decalcification solution is less than 0.5, it means that the decalcification i...

Embodiment 2

[0053] Select the femur or tibia of the animal, remove the muscle, periosteum and bone marrow, and obtain the tubular long bone part without cancellous bone in the middle part of the femoral shaft and below the tibial plateau. The bone segmentation machine cuts the bone canal into bone slices, and selects relatively flat bone slices as the material for tissue engineering menisci;

[0054] Use 1.5mol / L buffered hydrochloric acid decalcification solution for decalcification, use a pH meter to detect that the pH value of the decalcification solution is greater than 1.5 and replace the decalcification solution, the decalcification time is 90 days, stir the bone slices and decalcification solution with a shaker every day, Make the bone flakes fully contact with the decalcification solution;

[0055] After replacing the decalcification solution, measure the pH value of the decalcification solution for 5 consecutive days. If the difference between the pH value of the decalcification ...

Embodiment 3

[0063] Determination of expansion rate

[0064] 1) Freeze-drying the scaffolds of Examples 1 and 2

[0065] 2) Micro-weigh the fully dried stent and record it as W0

[0066] 3) Immerse in PBS (pH=7.4) solution and incubate at 37°C

[0067] 4) Take out the stent after 5 minutes, 10 minutes, 30 minutes, 1, 4, 12, 24, 48 hours,

[0068] 5) Use filter paper to remove the liquid sticking on the surface, and carry out micro weighing. Recorded as Wt

[0069] 6) Calculate the equilibrium expansion rate ESR (Equilibriumswellingratio) of the stent at each time point:

[0070] E S R = W t - W 0 W 0

[0071] The hydrated scaffold of Example 1 reached saturation in 8.9 minutes, and the hydrated scaffold of Example 2 reached saturation in 9.1 minutes. Both Examples 1 and 2 can quickly reach the e...

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Abstract

The invention relates to a preparation method of a meniscal scaffold. The meniscal scaffold is prepared from demineralized cortical bones of animals. The preparation method comprises the preparation steps as follows: pretreatment of animal femur or tibia, cutting into sections, decalcification, fixing, freeze drying, finishing of bone sections. The preparation method further comprises a step of hydration of the meniscal scaffold through a BMP-4 hydration solution. The meniscal scaffold after hydration can be applied to a tissue engineering meniscus transplantation process, the method has the advantages of easily available materials, a few steps and high preparation success rate, meniscus cells can grow into bone pores of the scaffold in the meniscus transplantation process, the transplantation success rate is increased, and the method has good industrial application prospect.

Description

technical field [0001] The invention designs a method for preparing a meniscus bracket, which belongs to the field of biological materials and tissue engineering. Background technique [0002] Loss of the meniscus in an animal or human often results in all kinds of severe pain and even immobility. The common clinical method is to use meniscus transplantation for treatment. [0003] Tissue engineered meniscus refers to a special meniscus tissue that is made of natural or synthetic organic or inorganic materials as a scaffold, and seed cells are added and cultivated under certain conditions. [0004] As the most basic and important part of the three elements of tissue engineering meniscus, meniscus support is the key to the success of tissue engineering meniscus. A good scaffold material should include the following important characteristics: compatibility with receptors, degradability, and the degradation time should match the progress of cell ingrowth, pores that allow cel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/50A61L27/56A61F2/38
CPCA61F2/30767A61F2/30942A61F2/3872A61F2002/3006A61F2002/30108A61L27/3608A61L27/3654A61L27/3687A61L27/3691A61L27/50A61L27/56A61L2430/06
Inventor 敖英芳谢兴朱敬先胡晓青
Owner PEKING UNIV THIRD HOSPITAL
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