Application of bacillus amyloliquefaciens B-1619 in aspect of promoting growth of greenhouse vegetables

A technology of B-1619, amylolytic spores, applied in the field of microorganisms, can solve problems such as serious soil-borne diseases, influence on vegetable quality and quantity, poor control effect of chemical agents, etc., and achieve the effect of increasing crop yield

Active Publication Date: 2016-07-13
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of the above problems, the present invention provides an application of Bacillus amyloliquefaciens B-1619 in promoting the growth of facility vegetables, so as to overcome the growing area of ​​facility vegetable planting and the increase of planting years, soil-borne diseases are becoming more and more serious, and the control effect of chemical agents Poor, cause facility eco-environmental pollution, the present situation that the quality and quantity of vegetables are caused more and more influence, the present invention is realized in this way:

Method used

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  • Application of bacillus amyloliquefaciens B-1619 in aspect of promoting growth of greenhouse vegetables
  • Application of bacillus amyloliquefaciens B-1619 in aspect of promoting growth of greenhouse vegetables
  • Application of bacillus amyloliquefaciens B-1619 in aspect of promoting growth of greenhouse vegetables

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1B-1619

[0018] The preparation of embodiment 1B-1619 bacterial liquid

[0019] Add 300ml of YPG culture solution into a 1000ml Erlenmeyer flask, transfer into a ring of activation (see Example 2 of patent 201210208366.9 for the activation method) and save Bacillus amyloliquefaciens B-1619 with the preservation number CGMCC No.4671 at 150rpm, 28 o C culture for 48 hours, the bacterial content in the bacterial solution reached 10 9-10 cfu / ml.

Embodiment 2

[0020] Example 2 Test of the growth-promoting effect of soaking seeds in different concentrations of B-1619 on tomato seed germination and root bud growth

[0021] Pick tomato seeds with the same size and full granules, treat them with 70% ethanol for 3 minutes, sterilize with 1% (V / V) sodium hypochlorite for 10 minutes, rinse with sterile water for 3 times, put 100 seeds per dish in a sterilized Petri dish In, add YPG culture solution, B-1619 bacterial solution obtained in Example 1, 10-fold dilution of B-1619 bacterial solution (diluted with water, the same below), 50-fold dilution, 100-fold dilution, and 500-fold dilution solution and 1000-fold dilution, while soaking seeds in sterile water as a control, soaking seeds at room temperature for 24 hours.

[0022] Then the seeds were placed in a petri dish with a layer of sterilized filter paper, 30 seeds were placed in each petri dish, repeated 3 times, and sterile water was added dropwise and placed in a light incubator for a...

Embodiment 3

[0028] The growth-promoting effect of embodiment 3 different concentrations B-1619 bacterium liquid on the growth of tomato seedlings

[0029] Pick tomato seeds with the same size and full particles, treat them with 70% ethanol for 3 minutes, then disinfect them with 1% (V / V) sodium hypochlorite for 10 minutes, rinse them with sterile water for 3 times, and then soak the tomato seeds in sterile water for 48 hours Then select the seeds with consistent germination and plant them in pots with 200 grams of sterilized soil, one seedling per pot, and add sterile water regularly during the cultivation period to ensure a constant volume of the hydroponic solution. After 10 days, water different concentrations of B-1619 bacterial solution and dilution (10 times dilution, 50 times dilution, 100 times dilution, 500 times dilution and 1000 times dilution), 50ml / pot, and set up YPG culture at the same time Liquid and sterile water were used as controls; after 30 days, water again according...

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Abstract

The invention discloses application of bacillus amyloliquefaciens B-1619 in aspect of promoting growth of greenhouse vegetables. 10-1000 times of diluent of B-1619 bacterium liquid with microbial content of 10<9-10>cfu/ml is watered to vegetables before sowing, in the seedling stage and after field planting, has a growth promotion effect on root length, fresh weight and dry weight of seedlings of greenhouse vegetables such as tomatoes or cucumbers, and has an effect of improving the total content of chlorophyll a, chlorophyll b and chloroplast pigments in plants and the content of carotene. Moreover, the crop yield can be obviously improved, and the bacterium liquid is suitable for large-scale popularization and application.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to the application of Bacillus amyloliquefaciens B-1619 in promoting the growth of facility vegetables. Background technique [0002] With the increase of the continuous planting years of protected vegetables, soil-borne diseases have become the main bottleneck of safe production of protected vegetables. At present, the prevention and control of soil-borne diseases in facility vegetables and continuous cropping obstacles mainly rely on spraying chemical pesticides, but the control effect is very unsatisfactory; at the same time, chemical control is likely to cause environmental pollution in facilities, increase the residual amount of toxic chemicals in facility vegetables, and bring harm to human health. Serious harm. Therefore, the strategy of using biological control technology to control soil-borne diseases and achieve the obstacle of ecological restoration of vegetable continuous ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01C1/00A01G7/06
CPCA01C1/00A01G7/06
Inventor 陈志谊陆凡杨晓云蒋盼盼甘颖
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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