Method for improving microbiological cultivation efficiency and fermentation efficiency by aid of straw extract liquid
A technology for microbial cultivation and fermentation efficiency, which is applied in the methods of using microorganisms, methods based on microorganisms, biochemical equipment and methods, etc., can solve the problems of low fermentation efficiency and slow growth of Rhizopus oryzae, and achieves simple process and fermentation efficiency. The effect of liquid enhancement and growth promotion
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment
[0024] a, crushing straw biomass to a particle size of less than 2mm; the straw is rice, corn or wheat straw;
[0025] The crushing method is ball milling or disc wet milling;
[0026] b. Put the straw biomass in step a into an aqueous solution; that is, an acid solution; or an alkali solution; or vibrate in ethanol or acetone;
[0027] c, after the reaction in step b is completed, centrifuge to take the supernatant; it is the straw extract; the temperature of the reaction is 10-180°C;
[0028] d, adding the supernatant obtained in the above step c, that is, the straw extract, into the culture medium; the volume ratio of the supernatant to the culture medium is 1: (1-20);
[0029] e, using the medium prepared in step d and adding straw extract as a direct fermentation medium; or as a pre-cultivation of microorganisms, stand-by;
[0030] f, using the pre-culture microorganisms obtained in the above step e to carry out production fermentation; the microorganisms are Rhizopus...
Embodiment 2
[0035] Example 2 Experiment of adding different concentrations of straw extracts in the fermentation medium
[0036] Experimental method: In this study, Rhizopus oryzae was applied as lactic acid producing bacteria. The condition of Rhizopus oryzae was 37°C, 150rpm, and the initial spore concentration was 10 5 pieces / ml. The medium components are 50g / L glucose, 0.125g / L (NH4) 2 SO 4 ,0.6g / LKH 2 PO 4 ,0.25g / LMgSO 4 7H 2 O, 2g / L yeast powder. The addition amount of straw extract was 0.1%, 7.5%, 10%, 50% (V / V). The concentrations of lactic acid, ethanol and glucose were determined by liquid chromatography. The detection equipment used is RI detector, and the chromatographic column is AminexHPX-87Hcolumn (7.8mmI.D.×30cm, Bio-Rad, USA). Oven temperature 65°C, mobile phase 5mMH 2 SO 4 , the flow rate is 0.6ml / min.
[0037] Experimental results: As shown in Figure 2, after adding straw extract to the medium, the glucose utilization rate in the three concentrations of 0.1...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More