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Tissue culture regeneration method of paeonia suffruticosa

A technology of tissue culture and peony, which is applied in plant regeneration, horticultural methods, botanical equipment and methods, etc., can solve the problems that peony traits are difficult to make a breakthrough and is in its infancy, and achieve the convenience of large-scale seedling planting, season and the effect of small environmental impact and fast seedling growth

Inactive Publication Date: 2016-07-20
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the breeding of peony varieties in my country is mainly carried out by using the hybridization model between varieties, which makes it difficult to make breakthrough improvements in the characteristics of peony.
The use of tissue culture technology can not only quickly breed peony plants, but also a new way to improve new varieties of peony. However, the research on tissue culture of peony is still in its infancy in China.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] The collection and processing of embodiment 1 explant material

[0036] Choose lush and disease-free plants, collect crab-yellow fruits in July, and let them dry in the shade indoors for 7 days to collect seeds, remove the shriveled seeds and impurities, and leave plump seeds. Put the seeds at 4°C for 90 days, break the dormant state of the epicotyls of the seeds, soak them in concentrated sulfuric acid for 2-3 minutes at 25°C, wash them with running water for 3-4 times, and then disinfect them with 20% sodium hypochlorite solution for 20 days Minutes, then wash 3-4 times with sterile water under sterile conditions, and dry the water with sterile filter paper for later use.

Embodiment 2

[0037] Example 2 Induction and differentiation of callus, regeneration of somatic embryos, differentiation of cotyledon embryos and induction of adventitious roots

[0038] 1) Induction and differentiation of callus:

[0039] The sterilized peony seeds obtained in Example 1 were inoculated in MSB medium, the culture temperature was 20-25° C., the light intensity was 20000 lux, and the light was 16 hours per day. Radicles appeared in 10 days. After 14 days, cut the radicle and place it in the induction medium (30g / L sucrose, 4.44g / L MSB, 40mg / L 2,4-D, 2g / L gel, pH 7.0), and the callus will appear after 15 days of culture Tissues, after 30 days of culture, large groups of callus and somatic embryos appeared.

[0040] 2) Somatic embryo regeneration:

[0041] Remove the somatic embryos with a smooth surface and yellow-green color from the callus mass to ensure that the bottom of each somatic embryo is connected with as little tissue as possible to minimize the differentiation of...

Embodiment 3

[0046] Embodiment 3 complete plant regeneration and seedling hardening

[0047]When the differentiated rooted explant grows to a plant body with a height of about 6 cm, the seedling is exposed to the air for hardening for 5-7 days. Then take it out, rinse the medium of the root, transfer it to a flowerpot containing moist vermiculite, pour nitrogen fertilizer once, and add water to moisturize every day. The control culture conditions are temperature 20-25°C, light intensity 20000 lux, and 16 hours of light per day. One week later, the plants were moved into the greenhouse field for cultivation. The cultivation conditions were: temperature 20-25°C, air humidity 85%, spraying a broad-spectrum fungicide every 7 days, and the survival rate reached 95% after hardening for 1 month. 10 pieces, 10cm high.

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PUM

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Abstract

The invention discloses a tissue culture regeneration method of paeonia suffruticosa. The method comprises the following steps: (1) performing soaking treatment on paeonia suffruticosa seeds with concentrated sulfuric acid, performing rinsing, and performing disinfection; (2) culturing the paeonia suffruticosa seeds so that radicles are produced, and then performing induction on callus tissues and somatic embryos; (3) performing regeneration on the somatic embryos so as to obtain secondary somatic embryo masses; (4) performing differentiation on the secondary somatic embryo basses so as to obtain cotyledon embryos; (5) performing further differentiation on the cotyledon embryos so as to obtain adventitious roots; (6) performing seedling hardening on explants forming the adventitious roots through differentiation, and forming complete plants. When the method disclosed by the invention is used, after seedling hardening is performed for one month, the survival rate of the plants can reach 95%, 8-10 leaves are produced, the height is 6-10 cm, and a large number of tissue culture seedlings can be obtained, so that planting needs are met. In addition, the method is high in seedling culture speed, high in efficiency and small in seasonal influence and environment influence, performing of large-scale seedling culture and planting at any time is facilitated, and the method has important application in the improvement of new species of the paeonia suffruticosa.

Description

technical field [0001] The invention relates to a method for tissue culture of flowers, which belongs to the field of plant tissue culture, in particular to a method for tissue culture and regeneration of peony. Background technique [0002] Peony (Paeoniasuffruticosa) is a perennial deciduous shrub of the genus Paeoniae in the family Ranunculaceae. The peony is beautiful in color and is known as the "king of flowers". In the cultivation type, it can be divided into hundreds of varieties mainly according to the color of the flowers. Peony is a unique woody and precious flower in China. It has a history of thousands of years of natural growth and more than 1,500 years of artificial cultivation. It is widely cultivated in China and has already been introduced all over the world. [0003] The methods of peony propagation include branching, grafting, and sowing, but most of them are branching and grafting, and the seeding method is mostly used to cultivate new varieties. At ...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01C1/00A01C1/08
CPCA01C1/00A01C1/08A01H4/00A01H4/001A01H4/008
Inventor 姚陆铭朱木兰王彪卢欣欣武天龙
Owner SHANGHAI JIAO TONG UNIV