Micro-cutting method for rapid propagation of Dalbergia odorifera seedlings

Abstract

The invention discloses a micro-cutting method for rapid propagation of Dalbergia odorifera seedlings. The method is as below: inoculating the seeds of Dalbergia odorifera into a bud induction culture medium and cultivating seedlings; cutting the obtained seedlings into stem segments, inserting the segments into a micro-cutting culture medium, and cultivating to grow lateral buds; cutting the lateral buds into stem segments, and inserting the stem segments into a fresh micro-cutting culture medium; cutting the obtained lateral buds into stem segments and inoculating the stem segments into a rooting culture medium, and cultivating for rooting, so as to obtain the Dalbergia odorifera seedlings. The process is simple and easy to operate, has rapid proliferation rate, resolves the problems of low germination rate and slow proliferation rate of seeds in seedlings breeding. The method achieves long-term, large-scale and rapid propagation of Dalbergia odorifera seedlings, can rapidly continuously obtain high-quality Dalbergia odorifera seedlings in a large amount and is practical.

Description

technical field [0001] The invention belongs to the field of agricultural biotechnology, and specifically relates to a method for rapidly propagating Dalbergia dalbergia seedlings by micro-cutting of test tube stem segments. Background technique [0002] Dalbergia dorifera (Dalbergiaodorifera), an endangered species, a specialty of Hainan, an evergreen tree of the Fabaceae Papilionaceae. Its wood is called "Huanghua pear", commonly known as "lower pressure wood", and it is called "Jiangxiang" in "Compendium of Materia Medica". The wood chips soaked in water can lower blood pressure and blood lipids. Superior material. Originally produced in Hainan Island, China, it is mainly distributed in Hainan, Guangdong, Fujian and other places in China. It is a national second-level key protected wild plant and an endangered tree species. The heartwood of Dalbergia Dalbergia is the most valuable. The heartwood is reddish-brown. The material is dense and hard. The texture is fine and b...

AI Technical Summary

Problems solved by technology

[0003] The traditional propagation method of Dalbergia japonica adopts seed propagation, and the existing problems of this method are: (1) there are few seed resources, and it is difficult to collect a large number of them; (2) the seeds are not resistant to storage, have a short life span, and cannot achieve regular sowing and seedling cultivation;

The existing problems of cutting propagation are: (1) the survival rate of cuttings is low, or even all die; (2) cutting resources are in short supply, and it is difficult to reproduce

Tissue culture currently adopts methods of inducing callus, differentiation and proliferating callus, and inducing adventitious shoots to take root to obtain sterile seedlings. The problems in this method are: (1) the callus induction time is long; (2) the callus The frequency of differentiated adventitious buds from wound tissue is low, and the variation rate of differentiated adventitious buds is high; (3) The elongation speed of callus differentiated adventitious buds is slow, and the time to reach the rooting standard is long

These problems have seriously hindered the large-scale production of Dalbergia balata seedlings, thereby unable to meet market demand