Application of composition in preparing drugs for treating tumors and using method of composition

A technology of tumor drugs and compositions, applied in the field of biomedicine, can solve problems affecting the quality of life of tumor patients, insensitivity, clinical chemotherapy and radiotherapy-sensitive tumor patient death, etc.

Inactive Publication Date: 2016-07-27
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the treatment options for clinical cancer patients are mainly surgical resection, chemotherapy and radiotherapy. Although with the remarkable achievements in clinical oncology research, the survival period of some cancer patients has been significantly prolonged and the quality of life has been continuously improved. The sensitivity of radiotherapy and its side effects are still an important cause of death in cancer patients
[0003] As one of the clinical first-line chemotherapy drugs for many tumors

Method used

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  • Application of composition in preparing drugs for treating tumors and using method of composition
  • Application of composition in preparing drugs for treating tumors and using method of composition
  • Application of composition in preparing drugs for treating tumors and using method of composition

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0017] Example 1: The anti-tumor effect of the pharmaceutical composition in vitro

[0018] First, by setting different concentration gradients (0.5μM, 1μM, 2μM, 4μM, 8μM, 16μM and 32μM) of cisplatin, the four head and neck cancer cell lines SCC4, SCC1, CAL27 and OSCC3 were treated. After 48 hours, the difference was detected by the CCK-8 experiment. The killing effect of the concentration of cisplatin, drawing the inhibition curve of tumor cell viability, and analyzing the half (50%) inhibitory concentration (ie IC50) of various cells (ie IC50) and IC25 and IC75 by Prism5 software, see Table 1:

[0019] Table 1 IC25, IC50 and IC75 of four head and neck cancer cell lines against cisplatin

[0020] μM

[0021] Use the corresponding IC50, IC25, and IC75 cisplatin concentrations of each cell line to treat the corresponding four types of cells, and set up three experimental groups: the first group is cisplatin alone treatment group: separate different concentrations of cisplatin Grou...

Example Embodiment

[0022] Example 2: Anti-solid tumor (head and neck cancer) effect of tumor chemotherapy drug composition in vivo:

[0023] Take 1*10 6 A head and neck cancer tumor cell line SCC4 with good growth in logarithmic growth phase was implanted in the armpit of nude mice of the BALB / cNude strain. After the tumor model is successfully established ( figure 2 A) According to the routine clinical use of cisplatin, the dosage is 75mg / m 2 , And the low-concentration cisplatin dose used is 10-20mg / m 2 , Take the average value as 15mg / m 2 In order to calculate the concentration used in mice corresponding to this clinical concentration, the following formula is required:

[0024] Mouse (mg / kg)*(mouse conversion factor / human conversion factor) = human (mg / m 2 ) / Person conversion factor

[0025] Among them, mouse mg / kg-mg / m 2 The conversion factor is 3, human mg / kg-mg / m 2 The conversion factor is 36. Converted into the clinical low-dose corresponding to the concentration of cisplatin used in mice is 5...

Example Embodiment

[0026] Example 3: Anti-solid tumor (head and neck cancer) effect of tumor chemotherapy drug composition in vivo:

[0027] Take 1*10 6 A head and neck cancer tumor cell line SCC4 with good growth in logarithmic growth phase was implanted in the armpit of nude mice of the BALB / cNude strain. After the tumor model is successfully established ( image 3 A) According to the findings of Example 2: Pretreatment of nude mice with 25 μg polyIC and then combined administration on the next day can significantly improve the killing effect of low-concentration cisplatin and reduce the toxic and side effects of this method. We further combined cetuximab, a commonly used targeted therapy for advanced head and neck cancer, to treat tumor-bearing nude mice, and set up three experimental groups ( image 3 B): The first group is the tumor-bearing nude mice group of the third treatment group in Example 2 (Poly(I:C) 25 / DDP 2.5 Group), the second group was pretreated with 25μg polyIC / mouse of tumor-bear...

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Abstract

The invention relates to a pharmaceutical composition for tumor treatment, in particular to a composition of tumor treatment drugs including the clinical first-line chemotherapy drug cisplatin, CDDP or DDP, artificially synthesized double-stranded RNA (polyinosinic-polycytidylic acid, poly IC or PIC) and the clinical targeting drug cetuximab, and the using dosage and method of the composition.In vitro and in vivo experiments both show that the composition has important significance for overcoming tumor chemotherapy-resistance and side effects thereof.

Description

Technical field: [0001] The invention relates to the field of biomedicine, in particular to a tumor chemotherapeutic drug composition as well as dosage and sequence. Background technique: [0002] According to the 2015 China Cancer Registration Annual Report, there were about 3.37 million new cancer cases in my country in 2011, an increase of 280,000 cases compared with 2010. In the past 10 years, the incidence and mortality of cancer in my country have shown a clear upward trend. At present, the treatment options for clinical cancer patients are mainly surgical resection, chemotherapy and radiotherapy. Although with the remarkable achievements in clinical oncology research, the survival period of some cancer patients has been significantly prolonged and the quality of life has been continuously improved. The sensitivity of radiotherapy and its side effects are still an important cause of death in cancer patients. [0003] As one of the clinical first-line chemotherapy drug...

Claims

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Application Information

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IPC IPC(8): A61K33/24A61K39/395A61K49/00A61P35/00A61P35/04C12Q1/02A61K31/713
CPCA61K33/24A61K31/713A61K39/395A61K49/00A61K49/0008G01N33/5011
Inventor 侯亚义丁亮泥艳红胡勤刚任靖张东亚历怡
Owner NANJING UNIV
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