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Helicobacter pylori hpaa subunit b cell epitope peptide and its application

A Helicobacter pylori epitope vaccine technology, applied in the field of medicine and biology, can solve the problems of single anti-Hp treatment plan and increased drug resistance of anti-Hp treatment plan

Active Publication Date: 2022-04-05
ZHEJIANG PROVINCIAL PEOPLES HOSPITAL
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Problems solved by technology

[0007] The purpose of the present invention is to provide a Helicobacter pylori HpaA subunit B cell epitope peptide and its application, which is conducive to the research and development of vaccines for the subsequent prevention and treatment of Hp infection, so as to solve the current single clinical anti-Hp treatment plan and drug resistance of anti-Hp treatment plan additional questions

Method used

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  • Helicobacter pylori hpaa subunit b cell epitope peptide and its application
  • Helicobacter pylori hpaa subunit b cell epitope peptide and its application
  • Helicobacter pylori hpaa subunit b cell epitope peptide and its application

Examples

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example 1

[0036] Example 1: Prediction of B-cell epitopes of Helicobacter pylori HpaA protein

[0037] 1. Gene sequence analysis and strain selection: Log in to the Genbank database (https: / / www.ncbi.nlm.nih.gov / genbank / ) to search for the nucleotide and amino acid sequences of Helicobacter pylori (Hp), and use DNA Star Megalign The software compares the nucleotide sequence and amino acid sequence of adhesin HpaA. According to the results of phylogenetic tree analysis, a suitable strain was selected as the source strain for HpaA protein immune epitope analysis. There are 7 nucleotide sequences of adhesin HpaA registered in NCBI, the length of the open reading frame is 783bp, encoding 260 amino acids. Such as figure 1 and figure 2 As shown, the nucleotide and amino acid homology of seven Helicobacter pylori adhesin HpaA are 93.5%-97.3% and 93.9%-98.1%, respectively. The results of phylogenetic tree analysis showed that the adhesin HpaA sequence of the CH-CTX1 strain was closer to th...

example 2

[0045] Example 2: Screening of a synthetic multi-antigen peptide vaccine and its dominant epitope vaccine based on candidate epitopes

[0046] 1. Synthesis of B cell epitope multi-antigen peptides: the synthesis of multi-antigen peptides was entrusted to Huaan Biochemical (Hangzhou) Co., Ltd. Based on the selected amino acid peptides and lysine as the core matrix, this example uses 8-branched peptides to design conformations and synthesize them on a polypeptide synthesizer by Fmoc solid-phase synthesis method. The polypeptides are purified by high-pressure liquid chromatography, and the purity is Reach more than 90%. For a schematic plan view of the preferred B cell epitope multi-antigen peptide, see Figure 13 . The 8-branch RPDPKRTIQKKSE constitutes MAP1, and the 8-branch GTDNSNDA constitutes MAP2.

[0047]2. The binding ability between MAP antigen peptide and Hp whole bacterial antibody: use different MAP vaccines with a concentration of 10 mg / L to coat 96-well enzyme-li...

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Abstract

The present invention provides a B-cell epitope peptide of Helicobacter pylori HpaA, the amino acid sequence of the B-cell epitope peptide is SEQ ID No: 1 and / or SEQ ID No: 2; a multi-antigen peptide list is also provided An epitope vaccine, the active ingredient of which is the above-mentioned B cell epitope peptide. Also provided is a medicine for preventing or treating Helicobacter pylori infection, the active ingredient of which is the above-mentioned B cell epitope peptide. It will be used in the development of vaccines for the prevention and treatment of Helicobacter pylori infection.

Description

technical field [0001] The invention belongs to the technical field of medicine and biology, and relates to a Helicobacter pylori HpaA subunit B cell epitope peptide and its application, which will be used in the development of a vaccine for preventing and treating Helicobacter pylori infection. Background technique [0002] Helicobacter pylori (Hp) is a microaerophilic, spiral-shaped pathogen that colonizes the gastric mucosa. Hp infection can induce gastrointestinal diseases such as chronic gastritis, peptic ulcer, gastric cancer and low-grade gastric mucosa-associated lymphoid tissue lymphoma, and is related to a variety of extra-gastrointestinal diseases, such as idiopathic thrombocytopenic purpura, iron deficiency Anemia etc. In 1994, WHO classified Hp as a class I carcinogen. In the 2015 Tokyo Global Consensus Report, Hp gastritis was defined as a severe infectious (infectious) disease, and Hp-positive patients should be eradicated unless there are countervailing fac...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/195A61K39/02A61P31/04
CPCC07K14/195A61K39/0208A61P31/04
Inventor 张骏孙艳李鹏何徐军董洁
Owner ZHEJIANG PROVINCIAL PEOPLES HOSPITAL
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