Method for inducing B12 botryococcus braunii to efficiently accumulate carotenoids by utilizing plant growth regulator EBR

The technology of a plant growth regulator and Botrytis brauneni is applied in the field of high-efficiency accumulation of carotenoids, which can solve the problems of increasing production costs, difficulty in technical popularization, complex fermentation technology and high requirements for production equipment, and achieves mature induction technology and production technology. Simple, Productive Efficiency

Inactive Publication Date: 2016-07-27
SHANDONG UNIV OF TECH
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  • Application Information

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Problems solved by technology

[0005] Although the above-mentioned patented technologies related to the purification and preparation of carotenoids are advanced, some of them may be polluted by heavy metals, such as copper, and the fermentation technology is relatively complicated, which requires high production equipment, which undoubtedly increases the production cost and technology. promotion difficulty

Method used

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  • Method for inducing B12 botryococcus braunii to efficiently accumulate carotenoids by utilizing plant growth regulator EBR
  • Method for inducing B12 botryococcus braunii to efficiently accumulate carotenoids by utilizing plant growth regulator EBR
  • Method for inducing B12 botryococcus braunii to efficiently accumulate carotenoids by utilizing plant growth regulator EBR

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Embodiment 1

[0014] Embodiment 1, adopt the following steps:

[0015] 1) Preparation of algae liquid: Under the conditions of 22°C, light intensity 2000lx and light-dark ratio of 12h / 12h, use 1 / 4 concentration BG11 medium to cultivate the strain of Botrytis branici B12 for 27 days to the logarithmic growth phase, and obtain For the algae liquid induced by EBR, add 1-fold concentration of BG11 nutrient salt every 14 days during the culture period;

[0016] 2) Accumulation of carotenoids: Take 200mL of algae liquid in the logarithmic growth phase and place it in a 300mL conical flask, then add EBR produced by Beijing Suo Laibao Technology Co., Ltd. to a working concentration of 0.01mg / L, at 2000lx, 24°C The induction treatment was carried out for 14 days. During the treatment stage, the algae were artificially shaken three times a day, and the time interval was 5 hours to induce the rapid accumulation of carotenoids in the algae liquid.

Embodiment 2

[0017] Embodiment 2, adopt the following steps:

[0018] 1) Preparation of algae liquid: Under the conditions of 24°C, light intensity 1800x and light-dark ratio 12h / 12h, use 1 / 4 concentration BG11 medium to cultivate the strain of Staphylococcus branici B12 for 28 days to the logarithmic growth phase, and obtain For the algae liquid induced by EBR, add 1-fold concentration of BG11 nutrient salt every 14 days during the culture period;

[0019] 2) Accumulation of carotenoids: Take 200mL algae liquid in the logarithmic growth phase and place it in a 300mL conical flask, then add

[0020] The EBR produced by Jingsuo Laibao Technology Co., Ltd. has a working concentration of 0.0095mg / L and is induced at 1800x23°C

[0021] For 15 days, during the treatment stage, artificial shake algae was performed 3 times a day, with an interval of 5 hours to induce the rapid accumulation of carotenoids in the algae liquid.

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Abstract

The invention provides a method for inducing a botryococcus braunii B12 strain to efficiently accumulate carotenoids by utilizing a plant growth regulator EBR. The method is characterized by adopting the following steps: 1) preparing an alga liquid culturing alga cells till the logarithmic phase by adopting 1/4 times concentration of BG11 nutritive salt under the conditions that the temperature is 23+/-2 DEG C, the light intensity is 1900+/-100lx and the light-dark ratio is 12h/12h, adding 1 times concentration of BG11 nutritive salt every other 14+/-1 days and shaking algae 3+/-1 times every day; and 2) accumulating carotenoids: putting 200ml of alga solution in the logarithmic phase in a 300mL conical flask, then adding EBR till 0.01+/-0.005mg/L and carrying out culture under the same conditions for 14+/-1 days and inducing carotenoids to be quickly accumulated. The technology is mature, simple and practicable, is low in cost, is efficient and pollution-free and can achieve the effect of obviously increasing the yield of carotenoids of the botryococcus braunii B12 strain in a short time.

Description

technical field [0001] The invention provides a method for efficiently accumulating carotenoids by using a plant growth regulator EBR to induce the botrytis brachyphylla B12 strain to accumulate carotenoids, and belongs to the field of biotechnology. Background technique [0002] Botryococcus braunii belongs to Xanthophyta, Xanthophyceae, Mischococcales, Botryococcaceae, and Botryococcus. It is a freshwater species distributed worldwide. unicellular microalgae. S. braziliana contains higher exopolysaccharides, fatty acids, especially hydrocarbons. In culture, its hydrocarbon production is 0.3%-76.0% of the dry weight of biomass, usually 25%-40%, and up to 86% in natural samples, which is much higher than the hydrocarbon content of other microorganisms (almost all less than 1%), the average calorific value of its hydrocarbon production is relatively high, and 1kg of grape hydrocarbon can be completely burned to release 3.0×10 4 ~4.2×10 4 kJ heat, atmospheric CO after comb...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P23/00C12R1/89
CPCC12P23/00
Inventor 孟春晓李国强郭艳芸高政权吴冠勋张瑞豪陈国强孙景涛崔建乐付永平肖潇王苗苗李邦高宇豪李贤博
Owner SHANDONG UNIV OF TECH
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