Method for enzymatically synthesizing medium-long chain structure triglyceride

A technology for structural triglycerides and long-chain triglycerides, which is applied in the field of enzymatic synthesis of medium and long-chain structural triglycerides, can solve the problems of high price of fatty acids and increase the difficulty of cost separation and operation, and achieves a simple process and is convenient for large-scale operations. The effect of industrialization promotion and high conversion rate

Inactive Publication Date: 2016-08-03
JIANGNAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the reaction needs to add excess fatty acid, and the high price of fatty acid will increase the cost and the difficulty of subsequent separation operations

Method used

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  • Method for enzymatically synthesizing medium-long chain structure triglyceride
  • Method for enzymatically synthesizing medium-long chain structure triglyceride
  • Method for enzymatically synthesizing medium-long chain structure triglyceride

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The batch enzyme reaction is carried out in a batch-type stirred tank reactor. The mass ratio of soybean oil and caprylic triglyceride is 6:4. In a solvent-free system, 8% immobilized lipase Lipozyme435 is added, and the pressure is 85°C Under stirring reaction 3h, stirring speed is 400r / min, after the reaction finishes, centrifuge 10min under the condition of 4000r / min to remove lipase, obtain medium and long chain structure triglyceride, through HPLC analysis, the content of medium and long chain structure triglyceride reaches 82.26%.

Embodiment 2

[0036] The batch enzyme reaction is carried out in a batch-type stirred tank reactor. The mass ratio of rapeseed oil and caprylic triglyceride is 1:1. In a solvent-free system, 5% immobilized lipase Novozym435 is added, under normal pressure Stir and react at 80°C for 4 hours at a stirring speed of 300r / min. After the reaction, centrifuge at 4000r / min for 10min to remove lipase and obtain medium-long-chain triglycerides. After HPLC analysis, the medium-long-chain structure triglycerides The content reaches 73.26%.

Embodiment 3

[0038] The batch enzyme reaction is carried out in a batch-type stirred tank reactor. The mass ratio of fish oil and caprylic triglyceride is 1:2. In a solvent-free system, add 10% immobilized lipase NS40086 and stir at 60°C under normal pressure React for 2 hours, stirring at a speed of 500r / min. After the reaction, centrifuge at 4000r / min for 10 minutes to remove lipase, and obtain medium and long-chain triglycerides. According to HPLC analysis, the content of medium and long-chain triglycerides reaches 65.82%. .

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Abstract

The invention discloses a method for enzymatically synthesizing medium-long chain structure triglyceride. Long-chain triglyceride and medium-chain triglyceride are mixed according to a certain mass ratio, lipase is added in a solvent-free system, stirring and reacting are carried out for a period of time at certain temperature, a transesterification reaction is carried out, and a product containing medium-long chain structure triglyceride is obtained. The method for enzymatically synthesizing medium-long chain structure triglyceride is high in reaction speed, high in conversion rate and mild in reaction condition, is efficient and fast, meets green chemistry and is huge in application potential, and large-scale industrial popularization is convenient.

Description

technical field [0001] The invention belongs to the technical field of oil synthesis, and in particular relates to a method for enzymatically synthesizing triglycerides with medium and long chain structures. Background technique [0002] Medium- and long-chain structural triglycerides (MLCT) are structural triglycerides synthesized by esterification or transesterification, and the fatty acids connected to the glycerol backbone contain both medium-chain fatty acids and long-chain fatty acids. Among them, medium-chain fatty acids refer to fatty acids with carbon numbers from C6 to C12, which mainly come from palm oil and coconut oil. It will be stored in adipose tissue and accumulated in the liver; the ketone body substances produced by metabolism can promote the immune function of the body; it does not depend on carnitine and directly enters the mitochondria for oxidative decomposition; its transport in the body does not require the participation of albumin. However, medium-...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64
CPCC12P7/6454
Inventor 王小三陆继源杨洁金青哲王兴国
Owner JIANGNAN UNIV
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