Establishment method of molecular diagnostic model of salivary protein fingerprint of gastric cancer with spleen deficiency syndrome
A fingerprint and salivary protein technology, which is applied in the field of establishing a molecular diagnostic model of saliva protein fingerprint of gastric cancer with spleen deficiency syndrome, can solve the problems of complex diagnosis of gastric cancer with spleen deficiency syndrome, long diagnosis time, low sensitivity, etc., and achieves good application prospects and strong specificity. , the effect of high sensitivity
Active Publication Date: 2018-11-06
SHENZHEN ELDERLY MEDICAL RES INST +1
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[0007] In view of this, the present invention aims to solve the problems of complex diagnosis of spleen deficiency syndrome of gastric cancer, long diagnosis time, low sensitivity and poor specificity in existing methods, and provides a method for establishing a molecular diagnostic model of gastric cancer spleen deficiency syndrome salivary protein fingerprint
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[0038] 1 case selection
[0039] 1.1 Diagnostic criteria
[0040] 1.1.1 Diagnostic criteria for gastric cancer
[0041] The diagnosis of gastric cancer is based on the diagnostic criteria of "Internal Medicine".
[0042] 1.1.2 Diagnostic criteria of spleen deficiency syndrome
[0043] (1) Spleen deficiency syndrome: spleen deficiency syndrome includes spleen deficiency and qi deficiency:
[0044] Main symptoms of qi deficiency: ① physical fatigue and weakness; ② mental fatigue and lazy speech; ③ pale tongue, fat tongue or tooth marks, thin white fur; ④ thready and weak pulse.
[0045] Main symptoms of spleen deficiency: ①decreased appetite or poor appetite; ②abnormal stool (loose, rotten, first hard and then loose, sometimes loose and sometimes hard); ③abdominal distension after eating, and abdominal distension in the afternoon.
[0046] Spleen-deficiency secondary symptoms: light mouth but not thirsty, like hot drinks, clear saliva in the mouth, continuous abdominal pain ...
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Abstract
The invention discloses a method for establishing a molecular diagnostic model of salivary protein fingerprints for gastric cancer spleen deficiency syndrome, comprising the following steps: sample collection; nano magnetic beads pretreatment; sample pretreatment; saliva sample elution and sample loading; mass spectrometry analysis; data collection; data analysis; building diagnostic models. The present invention uses WCX and MALDI‑TOF‑MS proteomics technology to carry out molecular diagnosis research on the combination of spleen deficiency syndrome and syndrome of gastric cancer based on salivary proteome, and finds that there are 6 differential protein peaks with statistical significance compared with spleen deficiency syndrome and non-spleen deficiency syndrome of gastric cancer . Compared with the normal control group with spleen deficiency syndrome in gastric cancer, a total of 106 different protein peaks were statistically significant, of which 101 protein peaks were significantly different, of which 26 mass peaks were down-regulated in the spleen deficiency syndrome group, and 80 mass peaks The expression was up-regulated in the spleen-deficiency syndrome group.
Description
technical field [0001] The invention belongs to the technical field of protein spectrum application, and in particular relates to a method for establishing a molecular diagnosis model of a salivary protein fingerprint for gastric cancer and spleen deficiency syndrome. Background technique [0002] Gastric cancer is a syndrome of deficiency in origin and excess in superficiality, with phlegm coagulation, stagnation of qi, blood stasis, and pathogenic heat as symptoms, and spleen deficiency as the foundation. Spleen and stomach weakness is the basis for the occurrence and development of gastric cancer, and exists in the entire process of gastric precancerous disease → precancerous lesion → gastric cancer. Insufficiency of the spleen can lead to internal growth of phlegm and fluid, and phlegm-damp retention can easily gather in the local area to form lumps, and phlegm and fluid can spread with the qi, pervading the body internally and externally, so that tumors are prone to rec...
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IPC IPC(8): G01N27/62
CPCG01N33/6848
Inventor 吴正治谢梦洲黄飞娟曹美群孙珂焕贺佐梅
Owner SHENZHEN ELDERLY MEDICAL RES INST