Desolvation and ionizationoun method through heating and apparatus

Abstract

The invention belongs to the technical field of mass spectrometric analysis and test, specifically to a desolvation and ionizationoun method through heating and an apparatus. The desolvation and ionizationoun method includes atomizing a sample to be tested, and heating the atomized liquid drops or particles of the sample through flame to be tested to evaporate solvent of ion sources and ionize neutral molecules. In this way, the gas phase sample ions are obtained. The apparatus comprises a sample atomizing device, a flame generator etc. The sample to be tested forms electriferous or neutral mist-like liquid drops at the outlet of an ion source sample atomizing device, solvent evaporation occurs to the electriferous or neutral mist-like liquid drops of the object to be analyzed when heated by flame to generate gas phase ions of which the mass spectrum can be detected, and proton-exchange reaction occurs to the neutral sample molecules and ions generated by flame to generate sample ions. The thermotropic solvent evaporation or proton-exchange reaction substantially improves the ionizationoun efficiency in the sample to be tested, the ion signal intensity of the sample is increased, and the sensitivity of mass spectrometry is effective improved.

Description

technical field [0001] The invention belongs to the technical field of mass spectrometry, and in particular relates to a method and device for desolvation and ionization, in particular to a method and device for heating desolvation and ionization of neutral molecules by using a flame as an ion source of a sample to be measured. Background technique [0002] Mass spectrometry is one of the most sensitive, efficient, and accurate analysis methods at present. The qualitative and quantitative analysis of the analytes can be realized by separating and detecting the analytes according to different mass-to-charge ratios (m / z). At present, it is widely used in modern scientific research and production activities, and plays an indispensable role in various trace or trace substance detection fields such as life science, food safety, environmental pollution detection, national defense security, aerospace, medicine, etc. It has become an indispensable analytical tool in the development ...

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Problems solved by technology

[0006] The above two methods can effectively achieve the desolvation effect, but their structures are relatively complicated. For the capillary heating method, a more complicated heating device is required, and the temperature is generally difficult to control. Too high or too low temperature is not suitable for some biological proteins. Molecules can be prone to denaturation and other issues

When using high-purity nitrogen for desolvation, the flow rate of the gas will affect the desolvation effect. If the flow rate is too low, the desolvation effect will not be obvious. If the flow rate is too high, the desolvation efficiency will increase, but it will reduce the concentration of the analyte. Ions enter the mass spectrometer inlet, directly affecting the detection sensitivity

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