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Application of baicalin in preparation of medicine for protecting heart development of fetus with pregnancy associated with diabetes

A fetal heart and diabetes technology, applied in drug combinations, sexual diseases, metabolic diseases, etc., can solve the problem of unclear medication for pregnant women and lactating women, and achieve the effect of alleviating embryonic heart dysplasia

Active Publication Date: 2016-08-17
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It has physiological effects such as antibacterial, diuretic, anti-inflammatory, anti-allergic, antispasmodic and strong anti-cancer response. It has occupied an important position in clinical medicine. At present, there are many manufacturers producing baicalin tablets, which are mainly used in acute and chronic diseases. Hepatitis, adjuvant therapy for persistent hepatitis, not clear for pregnant and lactating women

Method used

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  • Application of baicalin in preparation of medicine for protecting heart development of fetus with pregnancy associated with diabetes
  • Application of baicalin in preparation of medicine for protecting heart development of fetus with pregnancy associated with diabetes
  • Application of baicalin in preparation of medicine for protecting heart development of fetus with pregnancy associated with diabetes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1: A certain concentration of baicalin will not affect the development of chicken embryos

[0024] Early chicken embryos were cultured by the method of Early chick (EC) Culture, and the chicken embryos were divided into normal group (0.1% dimethyl sulfoxide DMSO) and baicalin group (DMSO dissolved baicalin). After baicalin was dissolved in DMSO, the concentration of DMSO in the working solution of the experimental group used was lower than 0.1%. After adding different concentrations of baicalin into EC Culture, chicken embryos were cultured to HH11 stage (0 μM: n=30, 3.125 μM: n=30, 6.25 μM: n=30, 12.5 μM: n=27 and 25 μM: n=30 ). It was found that 3.125 μM and 6.25 μM of baicalin had basically no significant effect on the embryonic development of 26h, 39h and 48h, which was similar to the morphology of embryos in the normal group (0μM) ( figure 2 A-C3 in ), the number of somites (the landmark structure for judging the early development stage of chicken embryo...

Embodiment 2

[0026] Example 2: Baicalin can significantly improve embryonic death and developmental deformities caused by high-sugar environment.

[0027] Based on the chicken embryo model of gestational diabetes we have established (Cell Cycle: 14(5):772-783.2015), we set up a normal chicken embryo group (0.1% DMSO), a high glucose group (50mM glucose), and a low concentration group of baicalin ( 6.25 μM), baicalin medium concentration group (6.25 μM) and 4 groups (n=10), culture chicken embryos in the 4 groups of EC Culture to HH11 stage, and conduct statistics on developmental stages and deformity rates. From image 3 In A-A1, we can observe that the embryos in the normal group develop well and have normal morphology; some embryos in the high glucose treatment group ( image 3 B-B3 in) Severe deformities were obviously observed at 48h (50%, image 3 B3 in), embryonic development lags ( image 3 In E), the logarithm of somites decreased compared with the normal group (p image 3 C-C3) ...

Embodiment 3

[0029] Example 3: Baicalin can significantly improve the abnormality of heart tube development caused by high glucose environment, and regulate the corresponding heart tube development genes.

[0030] The morphology of heart tubes in chicken embryos was observed by immunofluorescence with MF-20. We can see that the normal group and 6.25μM baicalin treated embryos in HH11 + The heart tube presents a C-shaped loop ( Figure 4 A-B in A-B), while the severely deformed embryos in the high-glucose treatment group had abnormal heart-tube fusion ( Figure 4In C), the recovered embryos treated with baicalin showed normal C-shaped circularization of the heart tubes. Then we detected the mRNA levels of the heart tube differentiation gene VMHC, the mesoderm adhesion gene N-Cadherin and the heart tube differentiation regulation gene BMP2 by RT-PCR. The mRNA level has no significant effect (p>0.05), but the high glucose environment will inhibit the expression of the above genes (p Figure...

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Abstract

The invention belongs to the field of traditional Chinese medicine, and particularly discloses application of baicalin in preparation of medicine for protecting heart development of a fetus with pregnancy associated with diabetes. A chick embryo test is conducted by adopting the traditional Chinese medicine monomer baicalin, and a result proves that a certain concentration of baicalin cannot cause chicken embryo development malformation and can effectively relieve adverse embryo heart development induced by a high glucose environment. According to the novel application of baicalin in the medicine field, the application range of baicalin is widened, the application value of baicalin is increased, and a contribution is brought for further development of new medicine, for example, the activity of baicalin is expected to be further improved or the side effects of baicalin are expected to be reduced by taking baicalin as a lead compound through structural modification or transformation.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicines, and in particular relates to the application of baicalin in the preparation of drugs for protecting fetal heart development in pregnancy complicated with diabetes. Background technique [0002] In recent years, with the improvement of people's living standards and changes in eating habits and lifestyles, the incidence of gestational diabetes has been increasing year by year around the world, becoming a public health problem in various countries. Glucose can pass through the placental barrier, and the fetal parenchyma of pregnancy complicated with diabetes is in a high-glucose environment. It has been reported that the incidence of congenital malformations of fetuses in diabetic pregnancy is 2-5 times higher than that in non-diabetic pregnancy, mainly manifested as congenital heart disease and neurological disorders. The system is abnormal. [0003] Patients with gestational diabetes ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7048A61P3/10A61P15/00
CPCA61K31/7048
Inventor 王广杨雪松陆大祥梁健欣高麟芮李帅钟山张诗瑶
Owner JINAN UNIVERSITY
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