A plant salt and a method of extracting the high-flavone plant salt from suaeda salsa by adopting neutral and alkaline proteases
The technology of neutral protease and Suaeda salsa is applied in the field of plant salt and the extraction of high flavonoid plant salt from Suaeda salsa using neutral and alkaline protease, which can solve the problem of no bacteriostatic effect and poor bacteriostatic effect. And other issues
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Embodiment 1
[0032] Embodiment 1 A kind of method that adopts neutral and alkaline protease to extract high flavonoid plant salt from Suaeda salsa
[0033] Include the following steps:
[0034] (1) cleaning
[0035] Select the tender stems and leaves of Suaeda salsa and the edible parts of cobs, cut them into sections, and the length is 5cm; put 30g of Suaeda salsa into the container, add cleaning water, and use 20KHz ultrasonic cleaning for 2 minutes at room temperature, and then use 40KHz Ultrasonic cleaning for 1 minute to clean the surface attachments of Suaeda salsa, and filter to remove impurities.
[0036] (2) dry
[0037] The cleaned Suaeda salsa is dried by centrifugal dehydration, and the moisture content after drying does not exceed 9-12% of the total mass of Suaeda salsa.
[0038] (3) Smash
[0039] The dried Suaeda salsa is crushed, and the particle size of the crushed material is 150-200 μm.
[0040] (4) Microwave broken wall
[0041] The above fine powder is placed at ...
Embodiment 2
[0058] Embodiment 2 Combination factor analysis experiment
[0059] The method described in Example 1 was used to extract plant salt, and only the addition of neutral protease, the enzymatic hydrolysis temperature of neutral protease, and the addition of alkaline protease in step (6) were changed for the experiment.
[0060] Table 1 Analysis experiment of combined factors of enzyme addition amount and enzymatic hydrolysis temperature
[0061]
[0062] Table 2 Index of extracted vegetable salt
[0063]
[0064] Through the above-mentioned factor combination experiment, it can be seen that embodiment 5 and embodiment 6 are preferred embodiments, that is, the addition of neutral protease is preferably 1.6-2.0%; the enzymatic hydrolysis temperature of neutral protease is preferably 46-50 ° C; alkaline The addition of protease is preferably 0.8-1.1%;
[0065] Preferred embodiment 5 and embodiment 6 are mixed with the solution of 20mg / ml, carry out antibacterial test, the re...
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