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Antioxidant Application of Dendrobium Leaf

A technology of dendrobium leaves and antioxidant activity, which is applied in the field of traditional Chinese medicine, can solve the problems of no antioxidative parts of dendrobium leaves and a preparation method thereof, and few dendrobium leaves, etc., and achieves the effects of abundant raw material sources, low price, and favorable development.

Inactive Publication Date: 2020-06-09
SHANGHAI JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, scholars at home and abroad have little research on Dendrobium leaves, only the research reports on leaf polysaccharides, leaf volatile oils and leaf flavonoid carbon glycosides, and no research reports on the antioxidant parts of Dendrobium leaves and their preparation methods

Method used

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  • Antioxidant Application of Dendrobium Leaf
  • Antioxidant Application of Dendrobium Leaf
  • Antioxidant Application of Dendrobium Leaf

Examples

Experimental program
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Effect test

Embodiment 1

[0020] Such as figure 1 As shown, this embodiment includes the following steps: dry the dendrobium leaves at 40°C, cut them into pieces, add water 10 times the weight of the medicinal material, reflux and extract 3 times, each time for 2 hours, filter while hot, combine the aqueous solutions, and concentrate the aqueous solutions to dryness under reduced pressure. It is the antioxidant active part of Dendrobium leaf.

Embodiment 2

[0022] Such as figure 1 As shown, this embodiment includes the following steps: dry the leaves of Dendrobium at 40°C, cut them into pieces, add water 12 times the weight of the medicinal materials, reflux and extract 3 times, each time for 2 hours, filter while hot, combine the aqueous solutions, and concentrate (concentrate to a volume equal to that of the medicinal materials). The total ratio is 5:1), the concentrated solution is centrifuged, loaded into a pretreated non-polar macroporous resin glass column, and eluted with 2-6BV distilled water (ie 0% ethanol) and 30% ethanol in sequence. The part eluted with 30% ethanol is concentrated to dryness under reduced pressure, which is the antioxidant active part of Dendrobium leaf.

Embodiment 3

[0024] Such as figure 1 As shown, this embodiment includes the following steps: dry the leaves of Dendrobium at 40°C, cut them into pieces, add water 12 times the weight of the medicinal materials, reflux and extract 3 times, each time for 2 hours, filter while hot, combine the aqueous solutions, and concentrate (concentrate to a volume equal to that of the medicinal materials). The total ratio is 5:1), the concentrated solution is centrifuged, and the concentrated solution is loaded into a pretreated medium-polar macroporous resin glass column, and 2 to 6 BV of distilled water (ie 0% ethanol), 25% to 35% ethanol, 60% ethanol elution. The part eluted with 60% ethanol is concentrated to dryness under reduced pressure, which is the part with antioxidant activity of the dendrobium leaf.

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Abstract

An antioxidation application of dendrobe leaf comprises: collecting dendrobe leaf, cutting it into pieces, adding water, reflux extracting, concentrating the extract solution, centrifugal processing, eluting by macroporous resin and concentrating under vacuum, finally removing solvent to prepare an edible additive. The antioxidation application of dendrobe leaf has a rich raw material source and a low cost; after extraction and purification of active components, the dendrobe leaf is beneficial to development of functional food.

Description

technical field [0001] The invention relates to a technology in the field of traditional Chinese medicine, in particular to an anti-oxidation application of dendrobium leaves. Background technique [0002] Free radicals are highly active oxygen molecules derived from normal metabolism of the body or exogenous factors such as: ionizing radiation, certain drugs, alcohol, smoking and other stimuli, which can attack unsaturated fatty acids in biological membranes, Oxidation, reduced membrane fluidity, loss of enzyme and receptor activity, and damage to membrane proteins lead to cell inactivation. Free radicals are associated with many diseases such as cancer, atherosclerosis, diabetes, neurodegenerative diseases and aging. Antioxidants can reduce the oxidative damage caused by free radicals to some important biological macromolecules, including proteins and DNA. In recent years, the research on antioxidants has attracted the attention of many scholars, especially the research ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C231/24C07C231/00C07C233/00A23L33/105
Inventor 李晓波彭崇胜高蓓蓓
Owner SHANGHAI JIAOTONG UNIV