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Moringa oleifera flavonoids with anti-complement and hypoglycemic activity, preparation method and application thereof

A kind of kinoleaf flavonoids, hypoglycemic technology, applied in the directions of medical preparations containing active ingredients, pharmaceutical formulas, allergic diseases, etc.

Active Publication Date: 2020-01-14
广东普莱健康食品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The Chinese invention patent with the patent number of 201310531168.0 has isolated relatively high-purity rutin and isoquercitrin monomer compounds from hops, and found that they have good antioxidant and anti-allergic effects; A report on flavonoid products with relatively pure active ingredients and good anti-complement and hypoglycemic activities isolated and purified from leaves

Method used

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  • Moringa oleifera flavonoids with anti-complement and hypoglycemic activity, preparation method and application thereof
  • Moringa oleifera flavonoids with anti-complement and hypoglycemic activity, preparation method and application thereof
  • Moringa oleifera flavonoids with anti-complement and hypoglycemic activity, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] (1) Moringa dried leaves are pulverized through a pulverizer, and cross 40 mesh sieves to obtain Moringa leaf powder;

[0042] (2)(2) Mix and stir the Moringa leaf powder and the extraction solvent according to the ratio of material to liquid 1:25 (units are g and mL respectively), and carry out ultrasonic extraction (70% ethanol volume concentration, 50°C extraction temperature, extract time 55min, ultrasonic power 100W), to obtain the extract, centrifuge to obtain the supernatant, carry out secondary extraction on the filter residue, centrifuge, and combine the supernatant; take the supernatant and carry out vacuum rotary evaporation at 50°C to obtain the concentrate.

[0043] (3) the concentrated solution is extracted successively with sherwood oil and ethyl acetate, and fat-soluble components such as chlorophyll, small polarity and medium polarity substances are removed successively; then extracted with n-butanol to obtain n-butanol extract, The n-butanol extract wa...

Embodiment 2

[0048] (1) Moringa dry leaves are pulverized through pulverizers, and cross 60 mesh sieves to get Moringa leaf powder;

[0049] (2) Mix and stir the Moringa leaf powder and the extraction solvent according to the ratio of material to liquid (1:35, the units are g and mL respectively), and carry out ultrasonic extraction (ethanol concentration 80%, extraction temperature 40°C, extraction time 70min, Ultrasonic power 150W) to obtain an extract, centrifuge to obtain a supernatant, perform secondary extraction on the filter residue, centrifuge, and combine the supernatant; take the supernatant and carry out rotary evaporation at 50°C to obtain a concentrate.

[0050] (3) the concentrated solution is extracted with sherwood oil and ethyl acetate successively to remove fat-soluble components such as chlorophyll, small polarity and medium polarity substances successively; then extract with n-butanol to obtain n-butanol extract, The n-butanol extract was concentrated by vacuum rotary ...

Embodiment 3

[0055] (1) Moringa dry leaves are pulverized through pulverizers, and cross 60 mesh sieves to get Moringa leaf powder;

[0056] (2) (2) Mix and stir the Moringa leaf powder and the extraction solvent according to the ratio of material to liquid (1:45, the units are g and mL respectively), and carry out ultrasonic extraction (60% ethanol concentration, 70°C extraction temperature, extract time 30 min, ultrasonic power 300W), to obtain the extract, centrifuge to obtain the supernatant, carry out secondary extraction on the filter residue, centrifuge, and combine the supernatant; take the supernatant and carry out rotary evaporation concentration at 50°C to obtain the concentrate.

[0057] (3) the concentrated solution is extracted with sherwood oil and ethyl acetate successively to remove fat-soluble components such as chlorophyll, small polarity and medium polarity substances successively; then extract with n-butanol to obtain n-butanol extract, The n-butanol extract was concen...

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Abstract

The invention discloses a preparation method and application of moringa oleifera leaf flavone with anticomplement and hypoglycemic activity. The preparation method includes: firstly adding moringa oleifera dried leaf powder into an ethanol solution to conduct ultrasonic extraction; using petroleum ether, ethyl acetate and n-butanol in order to conduct graded extraction on an extracted concentrated solution; carrying out pressure reduction concentration and vacuum freeze drying on the extracted solution to obtain powder A; dissolving the powder A in ethanol, passing the obtained product through a polyamide resin chromatographic column, using an ethanol solution to conduct gradient elution in order, and performing freeze drying to obtain powder B; dissolving the powder B in methanol, passing the obtained product through a Sephadex LH-20 chromatographic column, conducting methanol elution, collecting and combining the eluent, and carrying out concentration and freeze drying to obtain powder C; dissolving the powder C in methanol, using a preparative liquid phase reverse phase C18 column to conduct gradient elution, collecting the compound peaks eluted by 30%-35% methanol, and conducting concentrating and freeze-drying. The obtained flavone powder has purity up to more than 99%, and has strong anticomplement and alpha-glucosidase inhibitory activity.

Description

technical field [0001] The invention relates to a moringa leaf flavone, in particular to a moringa leaf flavonoid with anti-complement and hypoglycemic activities, a preparation method and application thereof. Background technique [0002] Moringa (Moringa oleifera Lam.), also known as drumstick tree, horse radish, undead tree, radish tree, etc., is a tropical deciduous woody vegetable and oil crop of Moringa family Moringa. It is native to the foothills of the Himalayas in northern India and Africa. It has a long history of planting in tropical and subtropical regions of Asia and Africa. There are 14 varieties in total. Currently, only traditional Indian moringa, improved Indian moringa and African moringa are cultivated for food. Three species of wood. In recent years, Moringa has been widely introduced and cultivated in Yunnan, Guangdong, Fujian, Taiwan and other places in my country, and the development of its products has also become a hot spot. [0003] Moringa is ri...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/185A61P3/10A61P37/02
CPCA61K36/185A61K2236/333A61K2236/39A61K2236/51A61K2236/55
Inventor 扶雄岳秀洁李超黄强
Owner 广东普莱健康食品有限公司