Preparation method and application of kelp extract for effectively reducing uric acid

A kelp extract and uric acid-lowering technology are applied in the field of biomedicine to achieve the effects of reducing serum uric acid levels, reducing expression levels, and protecting kidney function

Inactive Publication Date: 2016-09-07
GUANGDONG OCEAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the results of such studies are based on the inhibition of uric acid synthesis and metabolism as the main breakthrough, and the promotion of urate excretion under the premise of protecting kidney function has not been reported.

Method used

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  • Preparation method and application of kelp extract for effectively reducing uric acid
  • Preparation method and application of kelp extract for effectively reducing uric acid
  • Preparation method and application of kelp extract for effectively reducing uric acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] A preparation method for effectively reducing uric acid kelp extract, comprising the steps of:

[0038] (1) Take dried kelp, soak in distilled water at 4°C for 24 hours, and drain;

[0039] (2) Drain the kelp and cut it into pieces, add ethanol with a mass concentration of 50% according to the mass:volume ratio of 1:6, and fully homogenize;

[0040] (3) The kelp homogenate is subjected to ultrasonic treatment, the treatment time is 5s, the interval is 10s, and the frequency is 20 times;

[0041] (4) Stir and soak at 4°C for 48 hours;

[0042] (5) Vacuum the filtrate to 1 / 3 volume, concentrate by ultrafiltration, and the molecular weight cut-off of the ultrafiltration membrane is 300Da;

[0043] (6) The uric acid-reducing extract of kelp can be obtained by vacuum freeze-drying.

Embodiment 2

[0045] A preparation method for effectively reducing uric acid kelp extract, comprising the steps of:

[0046] (1) Take dried kelp, soak in distilled water at 4°C for 36 hours, and drain;

[0047] (2) Drain the kelp and cut it into pieces, add ethanol with a mass concentration of 50% according to the mass:volume ratio of 1:10, and fully homogenize;

[0048] (3) The kelp homogenate is subjected to ultrasonic treatment, the treatment time is 5s, the interval is 10s, and the frequency is 20 times;

[0049] (4) Stir and soak at 4°C for 72 hours;

[0050] (5) Vacuum the filtrate to 1 / 3 volume, concentrate by ultrafiltration, and the molecular weight cut-off of the ultrafiltration membrane is 3000Da;

[0051] (6) The uric acid-reducing extract of kelp can be obtained by vacuum freeze-drying.

Embodiment 3

[0053] A preparation method for effectively reducing uric acid kelp extract, comprising the steps of:

[0054] (1) Take dried kelp, soak in distilled water at 4°C for 30 hours, and drain;

[0055] (2) Drain the kelp and cut it into pieces, add ethanol with a mass concentration of 50% according to the mass:volume ratio of 1:8, and fully homogenize;

[0056] (3) The kelp homogenate is subjected to ultrasonic treatment, the treatment time is 5s, the interval is 10s, and the frequency is 20 times;

[0057] (4) Stir and soak at 4°C for 60 hours;

[0058] (5) Vacuum the filtrate to 1 / 3 volume, concentrate by ultrafiltration, and the molecular weight cut-off of the ultrafiltration membrane is 1000Da;

[0059] (6) The uric acid-reducing extract of kelp can be obtained by vacuum freeze-drying.

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Abstract

The invention relates to a preparation method and an application of kelp extract for effectively reducing uric acid, and belongs to the technical field of biological medicines. The preparation method particularly includes the steps: soaking dried kelps in 4 DEG C distilled water for 24-36 hours; draining and shearing the kelps; adding ethyl alcohol according to the mass-volume ratio of 1:6-10; sufficiently homogenizing the ethyl alcohol; stirring and soaking the kelps at the temperature of 4 DEG C for 48-72 hours after ultrasonic treatment; pumping filter liquor to 1 / 3 volume in vacuum; performing ultra-filtration concentration and vacuum freeze-drying to obtain the uric acid reducing kelp extract. By the aid of the kelp extract, the expression level of urate decorporation genes UAT can be remarkably improved, the expression level of urate reabsorption genes URAT can be reduced, and the content of serum uric acid is remarkably reduced on the premise of protecting renal functions.

Description

technical field [0001] The invention relates to a preparation method of a kelp extract, in particular to a preparation method and application of a kelp extract that can effectively reduce uric acid, and belongs to the technical field of biomedicine. Background technique [0002] Kelp, Phaeophyta, Neospora, Laminaria, Laminaceae, and genus Laminaria, is a large marine brown algae plant that grows in low-temperature seawater. It belongs to seaweed plants. It has large individuals, fast growth, and high economic value. It grows in sea water and gets its name because of its flexibility. Kelp is rich in algin and iodine, low in calories, medium in protein and rich in minerals. It is a vegetable with high nutritional value. It can extract iodine, algin, mannitol, etc., and has certain medicinal value. Studies have found that kelp has various biological functions such as lowering blood fat, lowering blood sugar, regulating immunity, anticoagulation, anti-tumor, detoxifying lead, a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/03A61P19/06
CPCA61K36/03A61K2236/33A61K2236/51
Inventor 刘华忠罗萍
Owner GUANGDONG OCEAN UNIVERSITY
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