Cyanine dye-nanogold SERS probe and preparation method thereof

A cyanine and nano-gold technology, applied in the field of nano-gold SERS probes, achieves good stability and enhanced SERS effect

Inactive Publication Date: 2016-09-07
SHANGHAI INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] There have been many studies on the use of different kinds of dye molecules as Raman reporter molecules on the surface of nanoparticles, but the self-assembly of the “π–π” interaction in the cyanine dye molecules on the surface of nanoparticles keeps the Raman reporter molecules of the dye molecules. The Mann reporter function simultaneously forms controllable nanoparticle small aggregates, and the construction of SERS probes with SERS hotspots has not yet been studied

Method used

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  • Cyanine dye-nanogold SERS probe and preparation method thereof
  • Cyanine dye-nanogold SERS probe and preparation method thereof
  • Cyanine dye-nanogold SERS probe and preparation method thereof

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Effect test

Embodiment 1

[0036] Cyanine dye molecule 1,1'-dibutyl-3,3,3',3'-tetramethylindocyanine iodide (C 31 h 41 IN 2 ) Preparation and characterization of 13nm gold SERS probe:

[0037] (1) Preparation of 13nm gold particles:

[0038] 1.7 mL of HAuCl 4The aqueous solution (1%) was mixed with 50 mL of water and heated while stirring until boiling, then 5 mL of sodium citrate solution (1%) was added rapidly, and the reduction reaction continued for 15 minutes. The solution was wine red at the end of the reaction, and was cooled to room temperature to obtain a gold nanoparticle sol with citrate ions on the surface, the particle size was ~13 nm, and the concentration was about 16 nmol / L.

[0039] (2) Surface plasmon characteristics of 13nm gold particles

[0040] figure 1 It is the UV-Vis spectrogram of 13nm gold particles, and it can be clearly observed that the maximum absorption wavelength (λ max ) at 519nm, its particle size corresponds to the 13nm particle size reported in the literature....

Embodiment 2

[0052] Cyanine dye molecule iodide 1,1'-dihexyl-3,3,3',3'-tetramethylindium cyanine (C 35 h 49 IN 2 ) Preparation and characterization of 30nm gold SERS probe:

[0053] (1) Preparation of 30nm gold particles:

[0054] 2.2 mL of HAuCl 4 The aqueous solution (1%) was mixed with 50 mL of water and heated while stirring until boiling, then 6.5 mL of acrylic acid solution (13%) (1%) was added rapidly, and the reduction reaction continued for 15 minutes. The solution was wine red at the end of the reaction, and was cooled to room temperature to obtain a sol of gold nanoparticles carrying acrylate ions on the surface, the particle size was ~30 nm, and the concentration was about 16 nM.

[0055] (2) Surface plasmon characteristics of 30nm gold particles

[0056] Figure 7 It is the UV-Vis spectrogram of 30 nanometer gold particles, and it can be clearly observed that the maximum absorption wavelength (λ max ) at 521nm, its particle size corresponds to the 30nm particle size rep...

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Abstract

The invention discloses a cyanine dye-nanogold SERS probe and a preparation method thereof. The nanogold SERS probe is composed of a gold nanoparticle with negative charges on the surface and a cyanine dye molecule carrying positive charges, wherein the gold nanoparticle with the negative charges is prepared from a negatively charged covering agent and an aqueous HAuCl4 solution through mixing, and the coverage rate of the cyanine dye molecule carrying the positive charges on the surface of the gold nanoparticle is 5 to 20%. The preparation method provided by the invention is simple; and the obtained nanogold SERS probe exerts strong hot spot effect in surface-enhanced Raman scattering (SERS) and has good stability.

Description

technical field [0001] The invention relates to the technical field of nano-gold SERS (surface-enhanced Raman spectroscopy) probes, in particular, designing a cyanine dye nano-gold SERS probe and a preparation method thereof. Background technique [0002] Although the synthesis, characterization and application of metal nanoparticles have been extensively studied by many researchers, the controllable synthesis and precise control of small nanoparticle aggregates is still a very interesting topic, because they are in the development of unique It is of great importance in the optical, electrical and biomolecular recognition properties of the material. Metal nanoparticles have been widely used in interdisciplinary fields, and one of the important classifications is based on gold nanoparticles as probes or carriers for therapeutic diagnosis, including pure gold, gold alloy, gold core-shell and other Gold nanostructures or nanocomposites are mainly due to the fact that their opt...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65
CPCG01N21/658
Inventor 成汉文罗谨马帅傅鸣春刘羽吕晶琦杨尧嘉李星菡
Owner SHANGHAI INST OF TECH
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