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Method using HPLC to measure ursolic acid content of haw lozenge

A technology of ursolic acid and hawthorn, applied in the determination of ursolic acid, HPLC determination of ursolic acid in hawthorn buccal tablets, to achieve the effect of simple detection method, strong specificity and good repeatability

Inactive Publication Date: 2016-09-07
WUZHOU INST FOR FOOD & DRUG CONTROL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

After reviewing the literature, there is no report on the content determination of its component ursolic acid

Method used

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  • Method using HPLC to measure ursolic acid content of haw lozenge
  • Method using HPLC to measure ursolic acid content of haw lozenge
  • Method using HPLC to measure ursolic acid content of haw lozenge

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Experimental program
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Embodiment 1

[0022] 1 Instruments and reagents

[0023] 1.1 Instruments Aglient 1260 biologically inert quaternary high-performance liquid chromatograph, Mettler-Toledo XA205DU electronic analytical balance from Germany.

[0024] 1.2 Sample: Caohetang hawthorn lozenges: produced by the Health Products Factory of Wuzhou Sanjian Pharmaceutical Co., Ltd., Guangxi (batch numbers 20120816, 20121217, 20120320); Negative samples: prepared according to the prescription and process of missing hawthorn. Ursolic acid reference substance was purchased from China National Institute for the Control of Pharmaceutical and Biological Products (batch number: 110742-200517, for content determination); other chemical reagents were of analytical grade.

[0025] 2 Methods and results

[0026] 2.1 Chromatographic conditions Chromatographic column phenomenonex Kinete x2.6u XB-C18100A column (100mm×4.6mm, 5μm); acetonitrile-0.1% phosphoric acid aqueous solution (68:32) as mobile phase; flow rate 1.0ml.min -1 ; T...

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Abstract

The invention discloses a method using HPLC to measure the ursolic acid content of a haw lozenge. The method has the advantages of simple operation and high specificity. The method comprises the following steps: preparing a control solution, preparing a test solution, precisely measuring 4 [mu]L of control solution and 4 [mu]L of test solution, injecting measured control solution and test solution into a high performance liquid chromatographic instrument, measuring the integral value of peak area, calculating the content by an external standard method according to the peak area, and drawing a standard curve. The invention belongs to the technical field of chemical detection.

Description

technical field [0001] The invention discloses a method for determining ursolic acid, in particular to an HPLC method for determining ursolic acid in hawthorn buccal tablets, and belongs to the technical field of chemical extraction. Background technique [0002] Hawthorn lozenges are made from hawthorn and white sugar. Hawthorn is rich in ursolic acid and other ingredients, which have the functions of soothing the liver and regulating qi, promoting blood circulation and removing blood stasis, and helping digestion. It is mainly used to treat symptoms such as fatty liver and liver fibrosis. In this study, the HPLC method was used to determine the content of the index component ursolic acid in Sanjiancao and Tangshanzha buccal tablets. After reviewing the literature, there is no report on the content determination of its component ursolic acid. Contents of the invention [0003] In view of the problems referred to above, the object of the present invention is to provide a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02G01N2030/027
Inventor 陈学松黄林杰韦韬林冬杰严飞龙
Owner WUZHOU INST FOR FOOD & DRUG CONTROL