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Biological compound fertilizer and preparation method thereof

A technology of biological compound fertilizers and cultures, applied in the direction of organic fertilizers, fertilization devices, fertilizer mixtures, etc., can solve problems such as unstable product quality and application effects, low overall level of the microbial fertilizer industry, and insufficient technological innovation, etc., to achieve Improve physical properties, enhance drought resistance, and improve the effect of soil aggregate structure

Inactive Publication Date: 2016-09-14
YIWU JINYU INFORMATION TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] Due to the long-term lack of investment in microbial fertilizer research in China, my country's microbial fertilizer industry still has problems such as low overall level, insufficient technological innovation, and unstable product quality and application effects.

Method used

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  • Biological compound fertilizer and preparation method thereof
  • Biological compound fertilizer and preparation method thereof
  • Biological compound fertilizer and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Bacillus subtilis (Bacillus subtilis) Li-2013-02, the strain has been preserved in the General Microbiology Center of China Committee for Culture Collection of Microorganisms (CGMCC for short) on July 15, 2013, the address is: Beichen West Road, Chaoyang District, Beijing, China No. 1 Courtyard No. 3), the preservation number is CGMCC No.7926.

[0030] The characteristic of the strain is that it has high enzyme activity for producing high temperature-resistant α-amylase, and strong heat resistance and acid resistance.

[0031] The high temperature-resistant α-amylase enzyme activity prepared by the strain is 30000-35000u / ml; the applicable temperature range is 105-115°C, the optimum reaction temperature is 110°C, and the enzyme activity is completely stable at 110°C; the applicable reaction pH range is 3.0-7.0, when the pH value is 3.0, the enzyme activity is completely stable, and the optimum reaction pH value is 4.2.

[0032] Described bacterial strain characteristic...

Embodiment 2

[0123] Preparation method of Bacillus subtilis culture:

[0124] Obtaining of fermented liquid: Obtaining Bacillus subtilis fermented liquid by step-by-step expansion culture of slanted strains;

[0125] (1) First-level seed cultivation: insert the slant strain of Bacillus subtilis into a 500-milliliter shake flask, with a medium loading capacity of 100 milliliters, a rotary shaker at 180 rpm, a cultivation temperature of 30° C., and a cultivation time of 24 hours;

[0126] (2) secondary seed cultivation: the primary seed is inserted into a 500 milliliter secondary seed shake flask according to the inoculum size of 10%, and the cultivation condition is the same as that of the primary seed;

[0127] (3) Tertiary seed cultivation: insert the secondary seed into a 5000 milliliter three-stage seed shaking flask with 10% inoculum size, 1000 milliliters of culture medium, 100 rpm of rotary shaker, 30° C. of cultivation temperature, and cultivate Time 24 hours;

[0128] (4) First-l...

Embodiment 3

[0139] The technical problem to be solved by the present invention is to overcome the deficiencies in the prior art and provide a biological compound fertilizer, the parts by weight of which are as follows: 10 parts of Bacillus subtilis culture, 8 parts of Aspergillus awamori culture, 20 parts of Lactobacillus plantarum mixed bacterial agent .

[0140] The parts by weight of the Lactobacillus plantarum mixed bacterial agent is 1 part of Lactobacillus plantarum CGMCC 7928;

[0141] Lactobacillus plantarum (Lactobacillus plantarum) CGMCC 11763 8 parts.

[0142] The bacterial classification that the present invention adopts is as follows:

[0143] Bacillus subtilis subsp CGMCC 7926

[0144] Lactobacillus plantarum (Lactobacillus plantarum) CGMCC 7928

[0145] Lactobacillus plantarum (Lactobacillus plantarum) CGMCC 11763

[0146] Aspergillus awamori CGMCC3.6484

[0147] The preservation unit of the above-mentioned strains is the China General Microorganism Strain Collection a...

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Abstract

The invention discloses a biological compound fertilizer, belongs to the field of fertilizers, and especially relates to biological fertilizers. The fertilizer comprises the components in parts by weight: 8-15 parts of a bacillus subtilis culture material, 5-10 parts of an aspergillus awamori culture material, and 10-20 parts of a lactobacillus plantarum mixed agent. The fertilizer can increase the grain yield, increases the vegetable yield, and also can improve the quality and flavor of vegetables and grains.

Description

technical field [0001] The invention relates to a biological fertilizer processing method, which belongs to the technical field of agricultural fertilizers, in particular to a biological compound fertilizer and a preparation method thereof. Background technique [0002] Fertilizer pollution has become a major public hazard in the world today. Agricultural experts have unanimously called for reducing the amount of chemical fertilizers to control the environment. An industry expert and academician Chen Wenxin of China Agricultural University believes that the excessive application of chemical fertilizers in my country has reached its limit. Taking nitrogen fertilizer as an example, my country's average application rate is nearly three times that of the United States and more than eight times that of Australia. This has caused a series of serious problems such as the destruction of soil structure, the reduction of natural biodiversity and ecosystem stability. Dr. Li Jun, dire...

Claims

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Application Information

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IPC IPC(8): C05F11/08C05G3/04C05G3/80
CPCC05F11/08C05G3/80
Inventor 李秉京
Owner YIWU JINYU INFORMATION TECH CO LTD
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