Lactobacillus pentosus for reducing cholesterol and nitrite and screening method thereof

A Lactobacillus pentosus and cholesterol-lowering technology, applied in the field of microorganisms, can solve the problems of Lactobacillus pentosus and other problems, and achieve the effects of improving the body's immunity, improving the intestinal flora, and reducing the residual amount

Active Publication Date: 2016-09-28
浅花堂健康科技(中国)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These Lactobacillus pentosus have certain practical applications, but so far, there is no application for Lactobacillus pentosus that has both the ability to lower cholesterol and the ability to degrade nitrite

Method used

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  • Lactobacillus pentosus for reducing cholesterol and nitrite and screening method thereof
  • Lactobacillus pentosus for reducing cholesterol and nitrite and screening method thereof
  • Lactobacillus pentosus for reducing cholesterol and nitrite and screening method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0026] Example Screening Lactobacillus pentosus GUFHSL-69 strain:

[0027] 1. Sample collection and strain isolation and purification

[0028] Rice flour products were collected from the Qiannan area of ​​Guizhou Province, 10g of the sample was put into 90mL of sterile peptone water, shaken on the shaker for 30min, and 200μL of the supernatant was spread on the CaCO3-MRS agar plate in the ultra-clean workbench, placed on Cultivate in a carbon dioxide incubator at 37°C for 48 hours, select a single colony that produces obvious calcium-dissolving circles for further purification, and then preserve the slant for future use.

[0029] 2. Colony morphology identification, physiological and biochemical identification and molecular biological identification

[0030] 2.1 The strain was cultured on the MRS plate for 24 hours, and the colony shape and color were observed and recorded.

[0031] 2.2 Gram staining: pick the colonies on the plate for smear, fixation, primary staining with ...

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Abstract

Lactobacillus pentosus for reducing cholesterol and nitrite is disclosed. The lactobacillus pentosus is separated from Guizhou traditional food fermented rice noodle. The strain is called lactobacillus pentosus GUFHSL-69 and was collected in the China Center for Type Culture Collection on January 4, 2016. The collection unit address is Wuhan University, Wuhan, China. The collection number is CCTCC M 2016001. Lactobacillus pentosus GUFHSL-69 is functional lactic acid bacteria, has good characteristics of reducing cholesterol and nitrite. The cholesterol removal rate is 25.66%, and nitrite degradation rate is 94.45% after 24h of culture. The strain is developed into a health-care food or drug which can improve intestinal flora, reducing serum cholesterol and boosting body immunity after long-term use. By the use of the strain, residual quantity of nitrite in a fermented product can be reduced, fermentation period is shortened, and flavor of a product is improved.

Description

technical field [0001] The present invention relates to microorganisms, more specifically to lactic acid bacteria, in particular to lactic acid bacteria with both cholesterol-lowering ability and nitrite-degrading ability. Background technique [0002] According to medical research, elevated cholesterol levels in serum are considered to be an important risk factor for cardiovascular diseases such as coronary heart disease and atherosclerosis. The World Health Organization (WHO) predicts that cardiovascular disease will remain the leading cause of death until 2030, affecting 23 million people. Clinical studies have shown that exceeding the normal serum cholesterol level (>5.2mmol) by 1mmol increases the risk of coronary heart disease by 35%. At the same time, reducing the serum cholesterol level by 1% will reduce the risk of disease by 2% to 3%. [0003] Studies have found that acute nitrite poisoning leads to methemoglobinemia, and chronic poisoning has the risk of carci...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/225
CPCC12N1/20C12N1/205C12R2001/225
Inventor 何腊平宋小娟李翠芹
Owner 浅花堂健康科技(中国)有限公司
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