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Detection method of traditional Chinese preparation for treating stomach illness

A technology of traditional Chinese medicine preparation and detection method, which is applied in the detection field of Liwei Capsules, can solve problems such as low accuracy, influence on peak area integration, and easy drift, etc., and achieve the goals of simple detection method, improved product quality, and reduced medication risk Effect

Inactive Publication Date: 2016-09-28
JIANMIN PHARMA GRP CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the spot color and separation degree of the qualitative identification results of Panax notoginseng in this patent are not very ideal. We have improved the qualitative identification method of Panax notoginseng
In the determination of the content of Panax notoginseng in the original patent, the active ingredient ginsenoside Rg of Panax notoginseng was determined by TLC scanning method. 1 Carried out content determination, this method has the shortcomings of large error, poor reproducibility, low accuracy, and difficult control of product quality
[0004] The mobile phase of the content in the 2015 edition of "Chinese Pharmacopoeia" and the literature related to the determination of the content of Panax notoginseng is a gradient mobile phase of acetonitrile and water. The baseline of this gradient mobile phase is prone to drift, which affects the integration of the peak area. Large; long analysis time, 65 minutes for a single sample

Method used

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  • Detection method of traditional Chinese preparation for treating stomach illness
  • Detection method of traditional Chinese preparation for treating stomach illness
  • Detection method of traditional Chinese preparation for treating stomach illness

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] The TLC identification of embodiment 1 Chinese medicine preparation of the present invention

[0046] The identification method of Panax notoginseng is: take a small amount of this product, add methanol for ultrasonic treatment, centrifuge, and take the supernatant as the test solution. Take a small amount of the reference medicinal material of notoginseng, add methanol for ultrasonic treatment, centrifuge, and take the supernatant as the reference medicinal material solution. Ginsenoside Rg 1 , notoginsenoside R 1 For the reference substance, add methanol to make a mixed solution containing 1mg per 1ml, as the reference substance solution. According to the test of thin-layer chromatography ("Chinese Pharmacopoeia" 2015 edition, the fourth general rule 0502), draw a small amount of the above three solutions, respectively point on the same silica gel G thin-layer plate, and use chloroform-n-butanol-methanol-water The lower layer solution is the developing agent, devel...

Embodiment 2

[0047] Example 2 Notoginseng saponin R in the traditional Chinese medicine preparation of the present invention 1 , Ginsenoside Rg 1 Assay method

[0048] Sample preparation method to determine:

[0049] The selection test of the extraction method investigated methods such as ultrasonic extraction for 30 minutes, reflux extraction for 30 minutes, and cold soaking for 12 hours. The results showed that the results of ultrasonic treatment were not much different from the results of heating and reflux extraction. Heptapenoside R 1 , Ginsenoside Rg 1 As a result, it was determined that ultrasonic treatment for 0.5h was used as the extraction method in this paper.

[0050] The choice of extraction solvent The test investigated the use of methanol, 50% methanol, ethanol, and 50% ethanol solution as the extraction solvent for ultrasonic extraction for 0.5h, and determined according to the method of content determination in the text. The results showed that when methanol was the ex...

Embodiment 3

[0078] Example 3 Ginsenoside Rb in the Chinese medicine preparation of Panax notoginseng of the present invention 1 content determination method

[0079]The selection of the preparation conditions of the test solution

[0080] Sample preparation method to determine:

[0081] The selection test of the extraction method investigated methods such as ultrasonic extraction for 30min, reflux extraction for 30min, and cold soaking for 12h. The results showed that the results of ultrasonic treatment were not much different from the results of heating and reflux extraction. Saponin Rb 1 As a result, it was determined that ultrasonic treatment for 0.5h was used as the extraction method in this paper.

[0082] Selection of extraction solvent The test investigated the use of methanol, 50% methanol, ethanol, and 50% ethanol solution as the extraction solvent for ultrasonic extraction for 0.5h, and determined according to the method of content determination in the text. The results show ...

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Abstract

The invention relates to a detection method of a traditional Chinese preparation for treating a stomach illness. The detection method of the traditional Chinese preparation comprises qualitative identification and content determination conducted on a monarch drug pseudo-ginseng, namely,pseudo-ginseng identification by adopting a thin-layer chromatography and content determination of notoginsenoside R1, ginsenoside Rg1 and ginsenoside Rb1 in the pseudo-ginseng by adopting high efficiency liquid chromatography. Compared with an existing quality control method, the quality control method has the advantages of being accurate and good in reproducibility. By adopting the method, the related medicine quality can be accurately controlled, the medicine usage risk can be reduced, and the product quality can be improved.

Description

technical field [0001] The invention relates to a detection method of a traditional Chinese medicine preparation for treating stomach diseases, more specifically a detection method of Liwei Capsules, which includes qualitative identification and content determination, and belongs to the field of modernization of traditional Chinese medicines. Background technique [0002] Liwei Capsules are scientifically combined with four medicinal materials such as Panax notoginseng, dry alum, calcined stamen, and wine rhubarb. Hemorrhagic gastritis, which has a significant clinical effect in the treatment of gastric diseases. [0003] Invention patent application CN 1879711A has disclosed a traditional Chinese medicine composition for treating stomach diseases and its preparation method and quality control method. However, the spot color and separation degree of the qualitative identification result of Panax notoginseng in this patent are not very ideal, and we have improved the qualita...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/90
CPCG01N30/02G01N30/90G01N2030/027
Inventor 李霞方胡彪黄志军吴木琴赵刚刘军锋熊登科任霞余丽花向阳朱立彬
Owner JIANMIN PHARMA GRP CO LTD
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