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Contrast amplifying support for the observation of a sample, production methods thereof and uses thereof

A technology for observing samples and supports, applied to instruments, optical components, coatings, etc., can solve problems such as difficult generalization

Active Publication Date: 2016-10-05
CENT NAT DE LA RECHERCHE SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Again, descriptions are made only for specific cases, which are difficult to generalize
Furthermore, in the case of the paper by M.A. Kats et al., the reflection is only partially eliminated

Method used

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  • Contrast amplifying support for the observation of a sample, production methods thereof and uses thereof
  • Contrast amplifying support for the observation of a sample, production methods thereof and uses thereof
  • Contrast amplifying support for the observation of a sample, production methods thereof and uses thereof

Examples

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Embodiment Construction

[0120] figure 1 shows a monochromatic parallel light beam FL (which can locally be regarded as a plane wave) of wavelength λ (in vacuum) perpendicularly incident on a structure consisting of a so-called incident semi-infinite medium MI, the beam originates from the incident semi-infinite medium that is transparent and characterized by the real refractive index n 0 ; The absorbent layer CA has a thickness e 1 , characterized by the complex refractive index N 1 =n 1 -jk 1 ("j" is an imaginary unit); and a so-called outgoing semi-infinite medium ME, which is transparent and characterized by a real refractive index n 3 0 . The incident medium may in particular be a substrate, for example made of glass, on which the layer CA is deposited. On the layer CA, on the exit medium side, it is possible to deposit 2 (or complex refractive index N 2 =n 2 -jk 2 ) samples (not shown). As explained above, in order to maximize the contrast of observing the sample, it is necessary to c...

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Abstract

The invention relates to a contrast amplifying support (SAC) for the observation of a sample, characterized in that it includes a transparent substrate (MI, SS) supporting at least one absorbing layer (CA), the complex refractive index N1=n1-jk1 and thickness of which are selected such that said layer behaves as an antireflection layer when it is illuminated with normal incidence and with an illumination wavelength l through said substrate, the surface of said layer opposite said substrate being in contact with a transparent ambient medium (AM) having an refractive index n3 that is lower than that of the refractive index n0 of said substrate. The invention also relates to a method for producing such a contrast amplifying support. The invention further relates to a method for observing a sample, or for detecting or assaying at least one chemical or biological species, using such a contrast amplifying support.

Description

technical field [0001] The present invention relates to contrast-enhancing supports for viewing samples, and methods for making such supports. The invention also relates to methods for observing samples and methods for detecting or analyzing chemical or biological substances using such supports. [0002] The present invention can be applied in various technical fields, such as biology (detection of biomolecules or microorganisms, observation of cell culture), nanotechnology (inspection of nano-objects, such as nanotubes), microelectronics, materials science, etc. Background technique [0003] The use of anti-reflection layers (or "λ / 4" layers) to increase the optical contrast of objects observed by reflection optical microscopy is a very powerful technique that has been known for many years; in particular, it led Langmuir and Blodgett in 1937 Molecular walks ( marches moléculaires ) were observed for the first time in 1999, and more recently, it also allowed Novoselov et al...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/45G01N33/50G02B1/11
CPCG01N33/54373G02B1/11G02B21/34G02B5/003G02B21/06G02B27/0012
Inventor D·奥塞尔C·安拉M·泽拉R·阿布哈奇夫
Owner CENT NAT DE LA RECHERCHE SCI