Production process for feed lipase with high stability and high activity

A technology with high stability and production process, applied in the direction of animal feed, immobilized on/in organic carrier, enzyme, etc., can solve the problems of decreased palatability, indigestion, feed caking, etc., to improve acid resistance and Effects of stability, accelerated decomposition, and rapid decomposition

Inactive Publication Date: 2016-10-12
昆明诺本科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Adding oil to the feed can improve the palatability and nutrition of the feed and reduce the dust generated in the feed, but most of the added oil is difficult to be absorbed by the gastrointestinal tract. Too much oil will cause waste of materials on the one hand, and on the other On the one hand, it can also cause diarrhea or indigestion in livestock, etc. The existing technology proposes to add lipase to the feed to promote the digestion of the fat in the feed for the lack of oil. The mechanical extrusion will generate a large amount of mechanical friction heat, which will lead to the inactivation of lipase. If pelleting is not carried out, the palatability of the feed will decrease, and there will also be problems such as agglomeration of oil and feed. To sum up, in feed production The process of directly adding lipase in the medium is not advisable under the existing technology
[0003] Technicians turn to add lipase to feed additives, and mix it with feed to eliminate the above problems. However, pure lipase is difficult to adapt to the acidic environment of the gastrointestinal tract. After being eaten by livestock, it has not yet reached the intestinal tract through gastric juice The point of action is almost inactivated, even if part of it is not inactivated and then digested by intestinal juice and bile, the final inactivated lipase is almost zero, and it is difficult to achieve the purpose of helping livestock digest oil in feed after eating lipase

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] A production process of lipase with high stability and high activity for feed, comprising trypsin modification, crude fiber expansion treatment, crude fiber solidification, and finished product processing, the specific steps are as follows,

[0016] a. Trypsin modification: lipase is modified by trypsin to obtain modified lipase;

[0017] b. Crude fiber expansion treatment: soak the dried crude fiber in water for 0.5h, take it out and drain it naturally until there is no moisture on the surface, put the treated crude fiber into a vacuum barrel for natural expansion treatment, and obtain large-pore crude fiber , cutting the macroporous crude fiber into 2cm long crude fiber fragments;

[0018] c. Crude fiber solidification: dissolve the modified lipase in water, soak the crude fiber fragments in the modified lipase solution for 0.5h, take out the crude fiber fragments and freeze-dry them to obtain solidified lipase, and modify the lipase in the modified lipase solution T...

Embodiment 2

[0021] A production process of lipase with high stability and high activity for feed, comprising trypsin modification, crude fiber expansion treatment, crude fiber solidification, and finished product processing, the specific steps are as follows,

[0022] a. Trypsin modification: lipase is modified by trypsin to obtain modified lipase;

[0023] b. Crude fiber expansion treatment: soak the dried crude fiber in water for 2 hours, take it out and filter it naturally until there is no moisture on the surface, put the processed crude fiber into a vacuum barrel for natural expansion treatment, and obtain large-pore crude fiber. The macroporous crude fiber is cut into 3cm long crude fiber fragments;

[0024] c. Crude fiber solidification: dissolve the modified lipase in water, soak the crude fiber fragments in the modified lipase solution for 0.5-5 hours, take out the crude fiber fragments, freeze-dry them to obtain solidified lipase, and modify the fat in the modified lipase soluti...

Embodiment 3

[0027] A production process of lipase with high stability and high activity for feed, comprising trypsin modification, crude fiber expansion treatment, crude fiber solidification, and finished product processing, the specific steps are as follows,

[0028] a. Trypsin modification: lipase is modified by trypsin to obtain modified lipase;

[0029] b. Crude fiber expansion treatment: soak the dried crude fiber in water for 3 hours, take it out and filter it naturally until there is no moisture on the surface, put the processed crude fiber into a vacuum barrel for natural expansion treatment, and obtain large-pore crude fiber. The macroporous crude fiber is cut into 5cm long crude fiber fragments;

[0030] c. Crude fiber solidification: dissolve the modified lipase in water, soak the crude fiber fragments in the modified lipase solution for 5 hours, take out the crude fiber fragments, freeze-dry them, and obtain solidified lipase. In the modified lipase solution, the modified lipa...

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PUM

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Abstract

The invention discloses a production process for feed lipase with high stability and high activity. The production process comprises the following steps: trypsase modification, coarse fiber swelling treatment, coarse fiber curing and finished product processing; fat produced by the process is subjected to enzyme modification while being subjected to physical curing protection, so that acid resistance and stability are further improved by adopting corresponding enzyme modification while stability and acid resistance of the fat are strengthened by physical curing; the enzyme medication can improve activity of inactivated fat lipase, so that decomposition of oil is quickened, and therefore, purpose of quickly decomposing oil in feed is achieved.

Description

technical field [0001] The invention relates to the technical field of using lipase in microbial secondary metabolites for processing feed additives, in particular to a production process of lipase for feed with high stability and high activity. Background technique [0002] Adding oil to the feed can improve the palatability and nutrition of the feed and reduce the dust generated in the feed, but most of the added oil is difficult to be absorbed by the gastrointestinal tract. Too much oil will cause waste of materials on the one hand, and on the other On the one hand, it can also cause diarrhea or indigestion in livestock, etc. The existing technology proposes to add lipase to the feed to promote the digestion of the fat in the feed for the lack of oil. The mechanical extrusion will generate a large amount of mechanical friction heat, which will lead to the inactivation of lipase. If pelleting is not carried out, the palatability of the feed will decrease, and there will al...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K20/189C12N11/12C12N9/20
CPCC12N11/12C12N9/20C12Y301/01003
Inventor 王春
Owner 昆明诺本科技有限公司
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