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Method for effectively killing viable but nonculturable state microorganisms generated in ultrasonic sterilization process

A technology of microbes and ultrasound, applied in the field of food engineering, can solve problems that are not involved, endanger food safety, etc., achieve the effect of promoting industrial application and optimizing the ultrasonic sterilization process

Inactive Publication Date: 2016-10-12
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, studies have shown that sonication can induce bacteria to form a viable non-culturable state
Live non-culturable bacteria cannot grow and reproduce normally on commonly used media, and can escape routine detection and cause false negative results, but still have vital signs and metabolic activity, and can recover and grow again under suitable conditions, and regain Infection ability has become a "hidden source of pollution" that endangers food safety, making the application of ultrasonic sterilization technology in the field of food processing a challenge
[0003] Effectively killing live non-culturable bacteria produced during ultrasonic treatment is an important means to improve the effect of ultrasonic sterilization and enhance the safety of ultrasonically processed food. However, the current application of ultrasonic in food sterilization is limited to evaluating the complete killing of ultrasonic Killed bacteria without reference to live, non-cultivable bacteria produced during sonication

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Ultrasonic combined with weak acid potential water treatment

[0024] (1) Preparation of bacterial suspension

[0025] The Staphylococcus aureus standard strain ATCC 25923 was inoculated in ordinary broth medium, and cultured on a shaker at 37°C until the logarithmic growth phase.

[0026] Experimental group: Wash the bacteria in the logarithmic growth phase twice with 0.85% sterile saline, and then resuspend the bacteria in 2mg / mL weakly acidic water, so that the concentration of the bacteria is about 10 8 CFU / mL, as the bacterial suspension of the experimental group;

[0027] Control group: Wash the bacteria in the logarithmic growth phase twice with 0.85% sterile normal saline, then resuspend the bacteria in 0.85% sterile normal saline, so that the concentration of the bacteria is about 10 8 CFU / mL, as the bacterial suspension of the control group;

[0028] (2) Ultrasonic treatment

[0029] Immediately process the suspension of Staphylococcus aureus with an ultra...

Embodiment 2

[0039] Ultrasonic combined with weak acid potential water treatment

[0040] (1) Preparation of bacterial suspension

[0041] Same as the method of step (1) in Example 1.

[0042] (2) Ultrasonic induction

[0043] The method of one step (2) in embodiment 1 is basically the same, only the processing time is changed to 10min.

[0044] Two detection

[0045] Same as the method of two in embodiment 1.

[0046]The results are as follows: the ratio of viable Staphylococcus aureus in the experimental group was 0.09%, the ratio of cultivable bacteria was 0.021%, and the ratio of live bacteria in non-culturable state was 0.069%. The ratio of viable Staphylococcus aureus in the control group was 89.40%, the ratio of cultivable bacteria was 43.65%, and the ratio of live bacteria in non-culturable state was 45.75%. It can be seen that the method of the present invention killed a large proportion of living non-culturable bacteria when the combined treatment was performed for 10 minute...

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Abstract

The invention discloses a method for effectively killing viable but nonculturable state microorganisms generated in ultrasonic sterilization process. The method comprises the following steps: staphylococcus aureus is washed with a stroke-physiological saline solution and then is swirled into slightly acid electrolyzed water; and the bacterial suspension is subjected to ultrasonic treatment immediately. According to the method provided by the invention, staphylococcus aureus bacteria are swirled into the slightly acid electrolyzed water, and then the ultrasonic treatment is carried out, so that the proportion of viable but nonculturable state bacteria is greatly reduced. The experiment of the invention proves that the technology of combining ultrasonic wave with the slightly acid electrolyzed water is used for treating bacteria, can effectively reduce the proportion of viable but nonculturable state bacteria in the ultrasonic sterilization process, enhances the safety of the ultrasonic sterilization process and is beneficial for promoting the industrial application of ultrasonic sterilization technology.

Description

technical field [0001] The invention relates to a method for effectively killing live unculturable microorganisms produced in the process of ultrasonic sterilization, which is beneficial to reduce the proportion of live unculturable bacteria, optimize the ultrasonic sterilization process, and further promote the industrial application of ultrasonic sterilization technology, belonging to field of food engineering technology. Background technique [0002] As an emerging non-thermal sterilization technology, ultrasonic sterilization has the characteristics of low energy consumption and short time. Compared with other non-thermal sterilization technologies, ultrasonic can effectively reduce the potential threat caused by sublethal cells in the process of killing microorganisms. Wastewater treatment, drinking water disinfection and other fields have been widely used. The use of ultrasound in food sterilization can not only effectively preserve food nutrients and natural color, a...

Claims

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Application Information

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IPC IPC(8): A23L3/005A23L3/358
CPCA23L3/005A23L3/358
Inventor 丁甜李娇廖新浴刘东红叶兴乾陈健初胡亚芹陈士国
Owner ZHEJIANG UNIV