A kind of mango core polyphenol extract and preparation method thereof
A mango core and extract technology, which is applied in food technology and biological fields, can solve the problems of high organic solvent residues and low total polyphenol content, and achieve the effects of large adsorption capacity, high desorption rate, and prevention of residues
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Embodiment 1
[0055] Embodiment 1: the preparation method of mango core polyphenol extract of the present invention
[0056] (1) Extraction of polyphenols from mango core: dry and pulverize the mango core, weigh 1 kg of mango core powder and add 20 L of ethanol with a concentration of 50%, heat reflux extraction three times, heat reflux extraction for 1 hour each time, combine the extracts, and concentrate the extracts under reduced pressure Juice extract, the extract is dissolved in water 10 times the weight of the extract, and the insoluble matter is filtered out to obtain a sample solution, the total polyphenol content is 18-20.9g / kg mango core powder.
[0057] (2) Loading: Put the mango core polyphenol extract on the column, control the flow rate of the effluent to 0.5BV / h, and the volume ratio of the sample amount to the resin AB-8 at 2:1 until the extract completely enters the resin bed.
[0058] (3) Elution: use 2-5BV of water for elution to remove impurities, then respectively use 3...
Embodiment 2
[0059] Embodiment 2: choose different conditions to extract
[0060] Adopt the method provided by the invention, select and carry out mango core polyphenols (sample is that mango core is dried and pulverized 1g) extraction experiment under different conditions, on the basis of single factor test, select ethanol concentration, solid-liquid ratio, extraction time and extraction temperature four main factor do L 9 (3 4 ) Orthogonal test (Table 1), the analysis of orthogonal results is shown in Table 2.
[0061] Table 1 Header design of orthogonal test for extraction process of polyphenols from mango core
[0062]
[0063] Table 2 Mango core polyphenol extraction process L 9 (3 4 ) Orthogonal experimental results
[0064]
[0065] Table 3 Mango core polyphenol extraction verification test
[0066]
[0067] It can be seen from Table 2 that the order of the influence of various factors on the extraction of mango core polyphenols is: B>A>D>C, that is, solid-liquid rati...
Embodiment 3
[0068] Embodiment 3: macroporous resin purification process
[0069] Select different resin types, sample concentration, adsorption rate, sample volume, ethanol elution concentration, elution rate and other factors to conduct single factor experiments to determine the optimal value of each factor.
[0070] Preparation of sample solution: Weigh 20 g of mango core powder, according to the ethanol concentration of 70%, the ratio of solid to liquid is 1:25, the extraction temperature is 60°C, each extraction is 1 hour, and the extraction is 3 times. After extraction, the filtrates are combined and concentrated to dryness under reduced pressure. Vacuum dry to constant weight, accurately weigh an appropriate amount of dry paste, dissolve in a 10ml volumetric flask with methanol or ethanol solution, add methanol or ethanol solution to dilute to the mark, shake well and set aside. Pipette 1ml of the filtrate to a 10ml volumetric flask, add methanol or ethanol solution to dilute to the...
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