Method for degrading triphenyl phosphate through brevibacillus brevis and application thereof

A technology of Bacillus brevis and triphenyl phosphate, applied in chemical instruments and methods, water treatment of special compounds, water pollutants, etc., can solve problems such as low efficiency, achieve low cost, good degradation effect, and environmental adaptability strong effect

Active Publication Date: 2016-10-12
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The microbial degradation of organophosphorus flame retardants is often not efficient in the actual application process, so the screening and application of efficient degradation bacteria of organophosphorus flame retardants has certain research significance

Method used

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  • Method for degrading triphenyl phosphate through brevibacillus brevis and application thereof
  • Method for degrading triphenyl phosphate through brevibacillus brevis and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] 1. Screening of degrading strains

[0024] (1) Collect the sediment in the water body of the polluted site, weigh 1g of the sediment sample, add it to the enrichment medium containing triphenyl phosphate with a concentration of 5mg / L, and cultivate it in a constant temperature shaker at 30°C and 150rpm. The main components of enrichment medium are: 5g / L beef extract, 10g / L peptone, 5g / L NaCl, 1000 mL distilled water, pH 7.0.

[0025] (2) Transfer once every 2 days, increase the concentration of triphenyl phosphate by 5mg / L at the same time of each transfer, until the concentration of triphenyl phosphate reaches 40mg / L, inoculate with 40mg / L triphenyl phosphate ester as the only carbon source in inorganic salt medium. The main components of the inorganic salt medium are: 1g / L NH 4 NO 3 , 1.5 g / L KH 2 PO 4 , 3 g / L K 2 HPO 4 , 40 mg / L triphenyl phosphate, 2mL / L trace element solution, 1000mL distilled water, pH 7.0. The composition of trace elements is: 4g / L MgSO ...

Embodiment 2

[0032] Example 2 Degradation analysis of Bacillus brevis to triphenyl phosphate

[0033] Inoculate Bacillus brevis into a 1000mL Erlenmeyer flask containing 500mL of sterilized enrichment medium, place it in a constant temperature shaker at 30°C, 150rpm, and cultivate for 24h. The main component of the enrichment medium is: 5g / L beef extract, 10g / L peptone, 5g / L NaCl, 1000 mL distilled water, pH 7.0.

[0034] Place the enriched cultured bacterial solution in a 50mL sterilized centrifuge tube at 6000 g After centrifugation at the speed of 5 min, the bacteria were collected and washed repeatedly with sterile normal saline and centrifuged for 3 times, and finally added normal saline to make a certain concentration (OD 600 =0.6) of bacterial suspension, add 1mL of prepared bacterial suspension into 19mL of sterilized degradation medium for degradation experiment, 30℃, 150rpm, after 5 days of incubation, the sample is determined by GC-MS, analysis Degradation effect of bacteria...

Embodiment 3

[0053] Same as Example 2, except that during the enrichment culture process, the pH of the enrichment medium is 6.5, the culture conditions are: the temperature is 20°C, the rotation speed of the shaker is 120 rpm, and the culture time is 16h, and finally Bacillus brevis triphenyl phosphate The degradation rate of ester was 94.8%.

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Abstract

The invention discloses a method for degrading triphenyl phosphate through brevibacillus brevis and application thereof and belongs to the technical field of environment organic pollutant biotreatment. The method specifically comprises the steps that a degradation culture medium with triphenyl phosphate as a unique carbon source is inoculated with the brevibacillus brevis under the condition that the temperature is 30 DEG C; five days after degradation of a constant-temperature shaking table, the concentration of the triphenyl phosphate left in the culture medium is measured through a gas chromatography-mass spectrometer (GC-MS), and in this way, the degrading effect of the brevibacillus brevis on the triphenyl phosphate is analyzed. According to the method, environment suitability of the method is high, and the cost is low; the degradation rate of organophosphorus fire retardant can reach 95.4% five days later. The actual application value is high, and a reference is provided for solving the problem of water organophosphorus fire retardant pollution treatment.

Description

technical field [0001] The invention belongs to the technical field of biological treatment of environmental organic pollutants, and in particular relates to a method for degrading triphenyl phosphate by using bacillus brevis and an application thereof. Background technique [0002] In recent years, brominated flame retardants (BFRs) have been gradually banned worldwide, and organophosphorus flame retardants (OPFRs) have gradually become popular due to their good flame retardant properties and low toxicity. Major substitute for BFRs, whose production and use have grown substantially. OPFRs are widely used in industries such as textiles, building materials, electronics, and chemical reagents. According to reports, the output of OPFRs in China reached 150,000 tons in 2013, and the annual growth rate is 15%. Since the way of adding OPFRs to materials is mainly direct addition rather than chemical bond synthesis, it is easy to be released into the environment during production ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34C02F101/30
CPCC02F3/34C02F2101/30C02F2305/06
Inventor 尹华唐少宇
Owner SOUTH CHINA UNIV OF TECH
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