LB solid culture medium preparing method

Abstract

The invention discloses an LB solid culture medium preparing method. The method comprises the steps of S1, adding tryptone, yeast extract and sodium chloride to water to be dissolved, adjusting pH to be 7.1-7.3 with sodium hydroxide aqueous solution, adding agar powder, conducting uniform mixing, and conducting volume metering to obtain solution A; S2, conducting high-pressure sterilization on the solution A obtained from S1, reducing temperature to 45-55 DEG C, adding antibiotics, and conducting uniform shaking to obtain solution B; S3, adding the solution B obtained from S2 into a culture dish, regulating air speed to be 800-1200 r / min, conducting blowing for 60-80 min, and sealing the culture dish with a sterile sealing film to obtain the LB solid culture medium. By the adoption of the method, generation of condensate water during LB solid culture medium preparation is avoided, the situation that the culture medium is polluted by bacteria is avoided, waste is reduced, the storage time of the culture medium is prolonged, the bacterial solution absorption capacity of the culture medium is improved greatly, test time is shortened, and test efficiency is improved.

Description

technical field [0001] The invention relates to the technical field of medium preparation, in particular to a preparation method of LB solid medium. Background technique [0002] LB medium is the name of a medium. In biochemical molecular experiments, this medium is generally used to pre-cultivate bacteria to multiply the bacteria and meet the requirements of use. It can be divided into liquid medium and solid medium. [0003] At present, the commonly used methods for preparing LB solid medium are: preparation of medium solution, sterilization, plating, solidification, and upside-down storage. The LB solid medium prepared by this method, because the medium cannot be completely solidified, after the plate is stored upside down, more condensed water will appear on the lid of the petri dish, resulting in inconvenient use, prone to bacterial contamination, and short storage life; Moreover, the ability of the solid medium to absorb the bacterial solution is relatively weak. When...

AI Technical Summary

Problems solved by technology

The LB solid medium prepared by this method, because the medium cannot be completely solidified, after the plate is stored upside down, more condensed water will appear on the lid of the petri dish, resulting in inconvenience in use, prone to bacterial contamination, and short storage life; Moreover, the ability of the solid medium to absorb the bacterial solution is weak. When doing experiments, it will take a long time to absorb the bacterial solution, which will delay the test process.