Steamed bread flavor substance detection method
A technology of flavor substances and detection methods, which is applied in the field of food flavor chemistry, can solve the problems of lack of basic and systematic research, and achieve the effects of low cost, improved extraction efficiency, and good reproducibility
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Embodiment 1
[0020] S1. Weigh a certain amount of steamed bread samples and ultrasonically treat them for 20 minutes at 35°C with an ultrasonic power of 800w;
[0021] It should be noted that the steamed bread samples were cut into 0.1cm 3 Cube, weigh 3g and carry out ultrasonic treatment;
[0022] S2. Put the ultrasonically treated steamed bread sample in S1 into a sample bottle, put the sample bottle into a 60°C constant temperature water bath, insert the extraction head into the sample bottle, push out the extraction fiber head, and extract the headspace part 50min;
[0023] The extraction head adopts a 65 μm PDMS / DVB extraction head;
[0024] S3. After the extraction is completed, insert the extraction head into the silica gel septum of the gas chromatography inlet of the gas chromatography / mass spectrometer, and analyze in splitless mode for 4 minutes;
[0025] Among them, the operating conditions of the gas phase are: the chromatographic column model is TR-5MS (30m×0.25mm×0.25μm),...
Embodiment 2
[0029] S1. Weigh a certain amount of steamed bread samples and ultrasonically treat them for 30 minutes at 35°C with an ultrasonic power of 1200w;
[0030] It should be noted that the steamed bread samples were cut into 0.5cm 3 Cube, weigh 5g and carry out ultrasonic treatment;
[0031] S2. Put the ultrasonically treated steamed bread sample in S1 into a sample bottle, put the sample bottle into a 60°C constant temperature water bath, insert the extraction head into the sample bottle, push out the extraction fiber head, and extract the headspace part 65min;
[0032] The extraction head adopts a 65 μm PDMS / DVB extraction head;
[0033] S3. After the extraction is completed, insert the extraction head into the silica gel septum of the gas chromatography inlet of the gas chromatography / mass spectrometer, and analyze in splitless mode for 5 minutes;
[0034] Among them, the operating conditions of the gas phase are: the chromatographic column model is TR-5MS (30m×0.25mm×0.25μm)...
Embodiment 3
[0038] S1. Weigh a certain amount of steamed bread samples and ultrasonically treat them for 25 minutes at 35°C with an ultrasonic power of 1000w;
[0039] It should be noted that the steamed bread samples were cut into 0.3cm 3 Cube, weigh 4g and carry out ultrasonic treatment;
[0040] S2. Put the ultrasonically treated steamed bread sample in S1 into a sample bottle, put the sample bottle into a 60°C constant temperature water bath, insert the extraction head into the sample bottle, push out the extraction fiber head, and extract the headspace part 58min;
[0041] The extraction head adopts a 65 μm PDMS / DVB extraction head;
[0042] S3. After the extraction is completed, insert the extraction head into the silica gel septum of the gas chromatography inlet of the gas chromatography / mass spectrometer, and analyze in splitless mode for 4.5 minutes;
[0043] Among them, the operating conditions of the gas phase are: the chromatographic column model is TR-5MS (30m×0.25mm×0.25μ...
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