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Dahurian larch LkANT gene, protein and applications

A larch, genetic technology, applied in the field of genetic engineering, can solve problems such as increasing the size of inflorescence rachis and flower organs

Active Publication Date: 2016-10-26
TIANJIN AGRICULTURE COLLEGE
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Problems solved by technology

Loss-of-function mutants of the ANT gene in Arabidopsis have smaller floral organs due to reduced cell numbers, however, overexpression of this gene increases the size of the inflorescence rachis and floral organs

Method used

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  • Dahurian larch LkANT gene, protein and applications
  • Dahurian larch LkANT gene, protein and applications
  • Dahurian larch LkANT gene, protein and applications

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Embodiment Construction

[0022] The present invention will be further described in detail below through the specific examples, the following examples are only descriptive, not restrictive, and cannot limit the protection scope of the present invention with this.

[0023] In the present invention, the larch transcriptome is sequenced and analyzed using Illumina / Solexa high-throughput sequencing technology. A 1465bp unigene was obtained through in-depth data mining and sequence splicing, and the unigene showed a high expression pattern in axillary buds. In order to deeply analyze the functional characteristics of the unigene, specific primers were designed according to the obtained unigene sequence information, and the unigene sequence was amplified in larch mRNA by RT-PCR technology. Using ORF Finder to analyze the open reading frame (ORF) of the unigene, it was found that the unigene contained an ORF of 1395bp, encoding a protein of 464 amino acids, and named it LkANT.

[0024] BLAST comparison analy...

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Abstract

A dahurian larch LkANT gene, a protein and applications are disclosed. The cDNA sequence of the gene is shown as SEQ ID NO:1. The protein encoded by the gene is characterized in that the amino acid sequence of the protein is shown as SEQ ID NO:2, and is characterized by applications of the protein in culture of multi-branch transgenic plants. By utilization of the modern molecular biological technique, the gene critical to regulation and control of branches and seed development for dahurian larch is cloned, separated and subjected to functional analysis. The acquisition of the critical gene of this type has important theoretical value, provides important candidate genes for increasing grain yields by utilizing genetic engineering means, and has an important application prospect in the field of crop genetic improvement and the like.

Description

technical field [0001] The invention belongs to the field of genetic engineering, in particular to a larch LkANT gene, protein and application. Background technique [0002] Branch development of higher plants is an important factor in determining plant morphogenesis, and is closely related to plant biomass and yield. The plant type of the aerial part of the plant is derived from the stem apical meristem during embryonic development and the lateral meristem during postembryonic development. Lateral meristems can differentiate to produce axillary buds, which further grow and develop to form lateral branches. The side branches are the same as the main stem, and also have the ability to form leaves, flowers, leaf axil meristems and secondary branches, but the branching situation is different in different species. Plant branch development is mainly regulated by multiple genetic factors, plant hormones and environmental signals. In recent years, through in-depth research on va...

Claims

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Application Information

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IPC IPC(8): C12N15/29C07K14/415C12N15/82A01H5/00
CPCC07K14/415C12N15/8261
Inventor 李爱于雪彭立新高英冯涛李慧于玮玮
Owner TIANJIN AGRICULTURE COLLEGE
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