EDTA antigen retrieval liquid
A repair solution and antigen technology, applied in the field of immunohistochemistry, can solve the problems of antigen destruction, volatile, drying of tissue slices, etc., and achieve the effect of good repair effect and non-volatile effect.
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Embodiment 1
[0027] An EDTA antigen retrieval solution, each 1000mL of EDTA antigen retrieval solution includes: Tris-Base 1.2114g, EDTA 0.37224g, Tween-20 500μl, triethanolamine 150mL and the remainder of single distilled water.
[0028] The preparation method is as follows: Weigh Tris-Base and EDTA according to the ratio, dissolve Tris-Base and EDTA with single-distilled water, add the formula amount of triethanolamine, add Tween-20 after complete dissolution, and finally dilute with single-distilled water to Rated Capacity.
Embodiment 2
[0030] An EDTA antigen retrieval solution, each 1000mL of EDTA antigen retrieval solution includes: Tris-Base 1.2114g, EDTA 0.37224g, Tween-20 500 microliters, ethylene glycol 325mL and the remainder of single distilled water.
[0031] The preparation method is as follows: Weigh Tris-Base and EDTA according to the ratio, dissolve Tris-Base and EDTA with single-distilled water, add the formula amount of ethylene glycol, add Tween-20 after complete dissolution, and finally dilute the volume with single-distilled water To rated capacity.
Embodiment 3
[0033] An EDTA antigen retrieval solution, each 1000mL of EDTA antigen retrieval solution includes: 1.20g Tris-Base, 0.35g EDTA, 400 microliters of Tween-20, 50mL triethanolamine and the remainder of monodistilled water.
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