Preparation method and application of a mycotoxin immunosensor based on graphene toluidine blue composite material

A technology of alkyne toluidine blue and immunosensor, which is applied to the analysis of materials, electrochemical variables of materials, instruments, etc., to achieve the effects of lowering the detection limit, ultra-sensitive and stable detection, and realizing chemical bond connection

Active Publication Date: 2017-11-07
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are many detection methods for mycotoxins, but most of them require large-scale equipment and professional operators

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Preparation method and application of a mycotoxin immunosensor based on graphene toluidine blue composite material

[0023] (1) with Al 2 o 3 Polish the glassy carbon electrode with a diameter of 4 mm to smooth, and clean it with ultrapure water; add 5 µL, 0.4mg / mL graphyne@toluidine blue Gy@TB composite material onto the electrode surface, rinse with ultrapure water, and leave at room temperature Dried down to form a film;

[0024] (2) Drop 3 µL of 0.1% hydroxypropyl chitosan on the surface of the electrode, rinse with ultrapure water, and dry at room temperature;

[0025] (3) Drop 1 µL of 0.1% glutaraldehyde aqueous solution on the surface of the electrode, rinse with ultrapure water, and dry at room temperature;

[0026] (4) Add 5 µL of mycotoxin antibody and 5 µL of bovine serum albumin BSA solution with a mass fraction of 0.5% to the surface of the electrode, rinse with ultrapure water, and dry in a 4°C refrigerator;

[0027] (5) Add 5 µL, 0.06 ~ 40 ng / mL of a ...

Embodiment 2

[0029] Preparation method and application of a mycotoxin immunosensor based on graphene toluidine blue composite material

[0030] (1) with Al 2 o 3 Polish the glassy carbon electrode with a diameter of 4 mm to smooth, and clean it with ultrapure water; add 7 µL, 3mg / mL graphyne@toluidine blue Gy@TB composite material dropwise to the electrode surface, rinse with ultrapure water, and leave it at room temperature dry film;

[0031] (2) Drop 6 µL of 2.5% hydroxypropyl chitosan on the surface of the electrode, rinse with ultrapure water, and dry at room temperature;

[0032] (3) Add 4 µL of glutaraldehyde aqueous solution with a mass fraction of 0.7% on the surface of the electrode, rinse with ultrapure water, and dry at room temperature;

[0033] (4) Add 7 μL mycotoxin antibody dropwise, 7 μL bovine serum albumin BSA solution with a mass fraction of 2% on the surface of the electrode, rinse with ultrapure water, and dry in a refrigerator at 4 °C;

[0034] (5) Add 7 μL, 0.06 ...

Embodiment 3

[0036] Preparation method and application of a mycotoxin immunosensor based on graphene toluidine blue composite material

[0037] (1) with Al 2 o 3 Polish the glassy carbon electrode with a diameter of 4 mm to smooth, and clean it with ultrapure water; add 8 µL, 4mg / mL graphyne@toluidine blue Gy@TB composite material onto the surface of the electrode, rinse with ultrapure water, and leave it at room temperature dry film;

[0038] (2) Drop 8 µL of 5% hydroxypropyl chitosan on the surface of the electrode, rinse with ultrapure water, and dry at room temperature;

[0039] (3) Drop 5 µL of 1% glutaraldehyde aqueous solution on the surface of the electrode, rinse with ultrapure water, and dry at room temperature;

[0040] (4) Add 8 µL mycotoxin antibody dropwise, 8 µL bovine serum albumin BSA solution with a mass fraction of 3% on the surface of the electrode, rinse with ultrapure water, and dry in a refrigerator at 4°C;

[0041] (5) Add 8 µL, 0.06 ~ 40 ng / mL of a series of my...

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Abstract

The invention relates to a preparation method and an application of a mycotoxin immunosensor based on a graphdiyne-toluidine blue composite material, and belongs to the technical field of novel biosensor sensing detection. Due to the sp hybridization structure of graphdiyne, graphdiyne has the advantages of no cis-trans isomer and high conjugation property. At the same time, the good [phi]-[phi] conjugation effect between toluidine blue and graphdiyne is utilized, the single molecules can be fixed firmly therefore, and the signal error caused by instable combination is reduced. So the provided mycotoxin immunosensor based on a graphdiyne-toluidine blue composite material can sensitively detect mycotoxin.

Description

technical field [0001] The invention relates to a preparation method and application of a mycotoxin immunosensor based on a graphyne@toluidine blue Gy@TB composite material. The graphyne@toluidine blue Gy@TB composite material is used as a detection base material to enhance conductivity while , and the signal molecule toluidine blue was successfully immobilized on the sensing surface. The use of hydroxypropyl chitosan not only stabilizes the base material, but also enables the antibody molecules to be successfully cross-linked on the sensing surface through glutaraldehyde, realizing the construction of a mycotoxin immunosensor, which belongs to new functional materials and biosensing detection technology field. Background technique [0002] Mycotoxins are metabolites produced by fungi growing in food or feed that are harmful to humans and animals. Eating foods contaminated with mycotoxins can lead to leukopenia and, in severe cases, cancer. Therefore, it is urgent to dete...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/543G01N27/26
CPCG01N27/26G01N33/54366
Inventor 任祥魏琴杜斌王耀光张勇孙旭范大伟
Owner UNIV OF JINAN
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