48 results about "Cis–trans isomerism" patented technology

The invention discloses a method for simultaneously identifying unsaturated lipid double-bond position isomerization, double-bond cis-trans-configuration and sn-position of a fatty acyl chain where double bonds are located, carbon-carbon double bonds are derived and converted into a product of an aza-ternary ring structure, and double-bond cis-trans isomers and sn-position isomers can be distinguished through liquid chromatography separation. The double-bond position in the double-bond position isomer can be judged through a tandem mass spectrometry method; the related aziridine acylation reaction is simple in reaction condition, fast in reaction and high in yield, and the problem of simultaneous identification of double bond position isomerization, double bond cis-trans isomerization and sn-position isomerization of a fatty acyl chain where double bonds are located of unsaturated lipid can be solved.

The invention discloses a separation method and application of CBDO cis-trans isomers, and relates to the technical field of compound isomer separation. The separation method comprises the following steps: respectively modifying hydroxyl groups of cis-CBDO and trans-CBDO to generate polarity difference, then separating the two isomers by a solvent dissolution-crystallization method, and finally removing protection to respectively obtain the cis-CBDO isomer and the trans-CBDO isomer. Through a modification protection-separation-deprotection method, the method has the advantages that the method is simple, the separation cost is reduced, and CBDO products with different cis-trans ratios and all-trans CBDO isomers can be obtained; the cis-trans ratio of the CBDO product can be up to 39.5 / 1, and compared with the commercially available CBDO, the copolyester prepared by using the CBDO product under the same condition has higher intrinsic viscosity and glass transition temperature, and the copolyester product with a full cis form can be obtained, so that the application range of the polymer can be expanded.

The invention discloses a pyrimidine compound as well as a preparation method, a pharmaceutical composition and application thereof. The structure of the compound is shown in a formula I, and the compound further comprises enantiomers, diastereoisomers, racemes, cis-trans-isomers, deuterated compounds, metabolites, prodrugs, solvates, hydrates, pharmaceutically acceptable salts or co-crystals of the compound. The compound has high-efficiency and high-selectivity inhibitory activity on various mutants of EGFR single mutation, double mutation and triple mutation, has low activity and low toxicity on wild type EGFR, and has a good treatment effect on cancers; the problems that an existing EGFR inhibitor is poor in effect, low in selectivity, resistant to drugs and the like are solved. # imgabs0 #

The invention belongs to the technical field of preparation of four-membered ring derivatives, and particularly relates to a four-membered ring derivative and a preparation method thereof. The preparation method of the four-membered ring derivative comprises the following steps: (1) adding two substrates, a photocatalyst and a solvent into a quartz tube, and stirring; (2) putting the quartz tube under ultraviolet light to carry out photocatalytic reaction; and (3) evaporating the reaction liquid to dryness, and separating through column chromatography to obtain the target compound. According to the invention, cis-olefin is used as an initial raw material to carry out cycloaddition reaction, so that the method has high regioselectivity and diastereoselectivity; the homodimerization reaction of olefin is effectively inhibited through reversible cis-trans isomerization reaction, so that the four-membered ring derivative is efficiently obtained. The four-membered ring derivative prepared by the invention plays a basic role in drug design, and provides a solid structural support for medical molecules.

The invention discloses a process method for synthesizing trans-3-hydroxy cyclobutane formic acid, which comprises the following steps: by taking 3-oxo-cyclobutane formate as a raw material, reducing carbonyl by adopting aluminum triisopropoxide under the catalysis of tri (pentafluorophenyl) borane to generate a cis-trans isomer with trans as a main component, and reacting with a solvent to obtain trans-3-hydroxy cyclobutane formic acid. The preparation method comprises the following steps: preparing a 3-hydroxycyclobutane formic acid cis-trans mixture, hydrolyzing in alkali to obtain the 3-hydroxycyclobutane formic acid cis-trans mixture, salifying and purifying the 3-hydroxycyclobutane formic acid cis-trans mixture and an alpha-methylbenzylamine compound to obtain trans-3-hydroxycyclobutane formic acid amine salt, and dissociating to obtain the trans-3-hydroxycyclobutane formic acid. According to the method, the selectivity of a trans-product during carbonyl reduction is improved, cis-trans isomers are separated in a salifying manner, single trans-3-hydroxycyclobutanecarboxylic acid is purified, the process conditions are mild, the operation is simple and convenient, and the method is suitable for process production.

The invention provides a branched-chain conjugated diene compound with insecticidal activity as well as a preparation method and application of the branched-chain conjugated diene compound. Specifically, provided are compounds of formula (I), optical isomers, cis-trans-isomers, or pharmaceutically acceptable salts thereof, the compounds have excellent and broad-spectrum insecticidal activity, and are very suitable for the development of low-toxicity, efficient, and environmentally friendly novel pesticides. # imgabs0 #

The application provides a method for regulating the crystal form and performance of polybutylene adipate based on cis-trans isomerization units, the crystal form of polybutylene adipate is regulated by introducing cis or trans units in the preparation process of polybutylene adipate, and the performance of polybutylene adipate is regulated by changing the introduction amount of cis or trans units; the introduction of cis or trans units is realized by adding cis-2-butene-1,4-diol or trans-2-butene-1,4-diol in the esterification or ester exchange reaction step. The application can effectively and simply change the crystal form of polybutylene adipate by introducing cis and trans units, thereby regulating the degradation rate and toughness of the material, realizing the preparation of polybutylene adipate material with controllable degradation rate and good toughness, and widening the application range of the material. The application does not change the original polymerization process, does not need to add additional additives and solvents, is environmentally friendly, and is conducive to realizing industrialized production.

The application discloses a piperitone cis-trans isomerization catalyst and a preparation method and application thereof. The catalyst is prepared by an impregnation reaction method from a noble metal precursor and a carrier precursor. The noble metal precursor accounts for 0.1-5% of the mass of the carrier precursor in terms of the noble metal contained. The application first uses a supported noble metal catalyst to perform an isomerization reaction under a hydrogen-free (inert) condition. The catalyst is simple to prepare and operate. The obtained supported noble metal catalyst has good catalytic activity in isomerization of cis-piperitone into trans-piperitone. The conversion rate of the cis-piperitone can reach 13.5%, the selectivity of the trans-piperitone can reach 100%, and the yield of the trans-piperitone can reach 13.5%.

The invention relates to the technical field of chemical separation, and discloses a separation method of cis-trans isomerized norrosewood, cis-trans norrosewood isomers are separated in high-speed counter-current chromatography through terpene-terpene DES, and a system with a separation effect on cis-trans isomerized norrosewood is designed through experiments. The method provided by the invention proves the feasibility of terpene-terpene DES on cis-trans isomer separation in high-speed counter-current chromatography, provides more choices for development of a new green solvent system, makes up for the vacancy of hydrophilic DES on separation of water-insoluble target objects in high-speed counter-current chromatography application, and can overcome the defects that a traditional organic solvent is large in use amount in the separation process, the separation time is short and the like. The residue is easy, and the environmental pollution is caused.

The present application relates to the technical field of organic chemical intermediates purification, in particular to a method and device for cis-trans isomerization conversion of 4-halo-2-methoxy imino acetoacetic acid ethyl ester. After cooling, 4-halo-2-methoxy imino acetoacetic acid ethyl ester is mixed with sodium chloride brine to form crystal slurry; then the crystal slurry is discharged into a crystal conversion kettle for aging; after aging, the crystal slurry is heated, extracted and centrifuged, and the organic phase is collected to obtain 4-halo-2-methoxy imino acetoacetic acid ethyl ester. Through the continuous process of "low-temperature brine crystallization enrichment-crystal conversion aging directional conversion-microwave rapid heating to prevent reverse conversion-two-stage extraction centrifugation purification-brine circulation to reduce cost", the content of cis-4-halo-2-methoxy imino acetoacetic acid ethyl ester is stably increased to 92-95%, and the production cost and environmental risk are reduced.

The invention provides a PN ligand capable of being subjected to cis-trans isomerization and a PN ligand modified Au cluster, and the PN ligand modified Au cluster with high photothermal conversion efficiency is prepared from the PN ligand capable of being subjected to cis-trans isomerization under illumination.

The application belongs to the technical field of tetra-cyclic ring derivative preparation, and particularly relates to a tetra-cyclic ring derivative and a preparation method thereof. The preparation method of the tetra-cyclic ring derivative comprises the following steps: (1) adding two substrates, a photocatalyst and a solvent into a quartz tube and stirring; (2) placing the quartz tube under ultraviolet light to perform a photocatalytic reaction; and (3) evaporating the reaction liquid and separating the target compound through column chromatography. The application uses cis-olefin as a starting material to perform a cycloaddition reaction, has high regioselectivity and diastereoselectivity, effectively inhibits homodimerization of the olefin through a reversible cis-trans isomerization reaction, and thus efficiently obtains the tetra-cyclic ring derivative. The tetra-cyclic ring derivative prepared by the application plays a basic role in drug design and provides a solid structural support for a pharmaceutical molecule.

Failures in establishing trust during international conflict negotiations frequently result in warfare, as ideological differences often cannot be resolved through rational discourse alone. Empathy between opposing parties is essential for building trust, and oxytocin is recognized for its critical role in facilitating empathy, trust, inter-brain synchrony, and cooperative decision-making. However, prevailing scientific studies typically do not differentiate between the cis and trans isomers of oxytocin, despite evidence that only the trans isomer is pharmacologically active. The present invention provides methods for the separation and quantification of oxytocin isomers, with particular emphasis on the trans isomer's function in social synchrony and decision-making within conflict negotiation contexts. Additionally, the invention explores the role of extremely low frequency electromagnetic radiation permeating the human central nervous system interconnecting neurochemical changes among groups. These advances clarify the unique neuropeptide functions of oxytocin and enhance understanding of its impact on cooperation and conflict resolution.

The invention relates to the technical field of organic matter detection, in particular to a 4-tert-butyl cyclohexanol cis-trans isomer separation and detection method and application thereof. Aiming at the separation problem caused by similar physicochemical properties of cis-isomers and trans-isomers, efficient separation of the 4-tert-butyl cyclohexanol cis-isomers and trans-isomers is successfully realized by adopting an RB-5 type chromatographic column under specific chromatographic conditions, and quantitative analysis is realized by combining an internal standard method. Experimental results show that the linear fitting degree of a standard working curve reaches 99.9% (R2), the adding standard recovery rate is 97.6%-102.3%, and the relative standard deviation (RSD) of repeated measurement (n = 5) is less than or equal to 1.8%, which indicates that the method has good repeatability and accuracy. The detection method not only solves the problem of separation of cis-trans isomers of 4-tert-butylcyclohexanol, but also has the advantages of simplicity and convenience in operation and short time consumption, and has higher practical application value.

The invention belongs to the technical field of enzyme engineering, and discloses a maleic acid cis-trans isomerase mutant, a genetic engineering strain, a method and application, the mutant is prepared by the following steps: by taking an amino acid sequence as shown in SEQ ID NO.1 as a parent sequence, mutating 124 alanine into valine to form a maleic acid cis-trans isomerase mutant A124V; the amino acid sequence of the maleate cis-trans isomerase mutant is as shown in SEQ ID NO.3, and the nucleotide sequence of the maleate cis-trans isomerase mutant gene is as shown in SEQ ID NO.4. The maleate cis-trans isomerase is mutated, the 124 site of the maleate cis-trans isomerase is mutated into valine, and the obtained mutant has better activity. The constructed maleic acid cis-trans isomerase mutant and fumarate synthase are subjected to coupled expression, the constructed genetically engineered bacterium takes maleate as a substrate, reaction is performed for 10-15 hours to produce L-malic acid, and the conversion rate can reach 99% or above.

The application discloses a preparation method of sulfentrazone intermediate, which comprises the following steps: in a solvent, substitution ring-closing reaction of cis-trans isomer compound (nitro hydrazone) shown in formula II and cyanate salt is carried out to generate sulfentrazone intermediate shown in formula III. The scheme provided by the application solves the problems in the prior art, such as the use of a large amount of inorganic reducing agent, such as sodium sulfite, in the production of raw material phenylhydrazine for preparing triazolinone through phenylhydrazine, generation of a large amount of solid waste, and low atomic economy; the scheme solves the problems in the prior art, such as the use of a large amount of sulfite reducing agent and sodium hypochlorite oxidant in the production of raw material phenylhydrazine for preparing triazolinone through phenylhydrazine, generation of a large amount of mixed salt, and low chloro-substitution selectivity of sulfentrazone prepared by using phenylhydrazine as raw material; the scheme solves the problems, such as the reduction of diazonium salt to hydrazine, the oxidation of hydrazine to hydrazine, and the like; the scheme directly utilizes intermediate hydrazine and then utilizes cyanate salt ring-closing, and compared with the prior art, the scheme is shortened by 3 steps, and energy consumption and labor intensity are greatly reduced.

The invention relates to a pyran compound as shown in a formula (I), and an isotope form, a stereoisomer, a tautomer, a cis-trans-isomer, a pharmaceutically acceptable salt, a pharmaceutically acceptable solvate, a hydrate, a prodrug and a polymorphic substance of the pyran compound. The compound shows high inhibitory activity and high selectivity, so that the compound has a good application prospect in the aspect of preparing medicines for treating steroid receptor dependent diseases.

The present invention relates to a Bacillus licheniformis host cell for increased production of secreted proteins. Specifically, the invention relates to a Bacillus licheniformis host with a genetic modification that leads to increased production of a heterologous, non-native, secreted enzyme. Specifically, the present invention relates to a Bacillus licheniformis host cell expressing a) a first polypeptide having peptidyl-prolyl cis-trans isomerase (EC 5.2.1.8) activity, said first polypeptide comprising an amino acid sequence as shown in SEQ ID NO: 1, or an amino acid sequence being a least 81% identical to SEQ ID NO: 1, and b) a second polypeptide having amylase activity, wherein said second polypeptide is heterologous to said Bacillus licheniformis host cell.

The invention discloses a method for synthesizing an amatoxin intermediate through Rh / Cu concerted catalysis, and belongs to the technical field of allylic alkylation reaction, an Rh / Cu concerted catalysis system is used for carrying out chiral selectivity and stereoselectivity allylic alkylation reaction on p-diphenyl imine glycine tert-butyl ester and racemic carboxylic acid allyl ester, and the amatoxin intermediate is synthesized. The rhodium catalyst controls the configuration of electrophilic carbon atoms in allyl ester, the copper catalyst controls the configuration of nucleophilic carbon atoms in unstable enol salt, and finally the amatoxin intermediate is obtained. According to the method for synthesizing the amatoxin intermediate through Rh / Cu concerted catalysis, an Rh / Cu concerted catalysis system can achieve ortho-position three-level three-dimensional center construction, multiple aryl and aliphatic substituted branched chain allyl products are obtained through the Rh / Cu concerted catalysis system, the yield is good or higher, cis-trans isomerization selectivity is high, and the method is suitable for industrial production. The enantiomeric selectivity is good to excellent.

The application discloses a cis-trans cyclopropane diacid diethyl ester nondestructive detection method based on feature screening, relates to the technical field of testing or analyzing materials by means of determining the chemical or physical properties of the materials, and comprises the following steps: taking a plurality of mixtures formed by cis isomers and trans isomers of cyclopropane diacid diethyl ester respectively at a plurality of mass ratios, and taking Raman characteristic peak parameters of the plurality of mixtures; based on the Raman characteristic peak parameters of the plurality of mixtures, a plurality of Raman characteristic peak proportional type variables between cis-trans isomers are constructed; a prediction model is constructed; and based on the prediction model, the mass ratio of the cis-trans isomers of cyclopropane diacid diethyl ester is predicted; the method has the advantages of rapidness, nondestructiveness, non-separation, high recognition precision and the like, and is suitable for the fields of isomer component analysis, structure identification and precise quantitative detection.

A compound as represented by formula I, or an optical isomer, a cis-trans isomer or a pharmaceutically acceptable salt thereof, and a preparation and the use thereof. The compound as represented by formula I can provide polymyxin a significant synergistic effect with regard to resisting various gram-negative bacteria, and can be applied to the treatment of infections caused by polymyxin-insensitive bacteria or drug-resistant bacteria.

The present invention relates to novel compounds useful as sensors for the rapid and specific determination of the FKBP12 protein, a peptidyl-prolyl cis-trans isomerase (PPlase), the levels of which in the biological fluids of a subject change if the subject is affected by pathological conditions, in particular neurodegenerative diseases, such as the Parkinson's disease and the Alzheimer's syndrome, tumour pathologies, autoimmune diseases, or if that subject is in a phase of acute rejection after organ transplantation.

The present invention relates to the technical field of molecular-based magnetic materials. In order to solve the problem that the synthesis of existing asymmetric lattice-type spin-crossover materials is difficult and it is difficult to achieve an asymmetric arrangement of coordination sites by synthesizing symmetrical multidentate ligands, a lattice-type iron-based complex based on cis-trans isomerized triazoles and a preparation method thereof are proposed. The spin-crossover material prepared by the present invention is based on a bidentate triazole ligand, and a lattice-type spin-crossover material is prepared by self-assembly of the ligand and transition metal ions; by adjusting the terminal substituent, the orientation of the ligand can be well adjusted to achieve the assembly of an asymmetric lattice-type spin-crossover material. The experimental operation of the present invention to prepare a bidentate triazole ligand by condensing a cyano compound with a hydrazide and then dehydrating it is simple, the yield is as high as more than 85%, and it is easy to promote and apply on a large scale.

The invention discloses a co-production method for separating CBDO cis-trans isomers and calcium phthalate and application, and relates to the technical field of compound isomer separation. The separation method comprises the following steps: reacting a cis-trans isomer mixture of CBDO with phthalic anhydride to obtain carboxyl-terminated CBDO, then separating the two isomers by a solvent dissolution-filtration method, and finally carrying out alkaline hydrolysis on a product by using a calcium oxide or a calcium hydroxide. Further, a cis-CBDO isomer, a trans-CBDO isomer and calcium phthalate are respectively obtained. According to the method, the alkaline compound of calcium is used, alkaline hydrolysis of carboxyl-terminated CBDO is achieved, CBDO is regenerated, the recovery rate of CBDO is larger than 90%, the byproduct calcium phthalate with the high additional value is obtained, no three wastes are discharged in the separation process, and CBDO products with different cis-trans ratios can be obtained; and the cis-trans ratio of the CBDO product can be up to 97 / 3.

The present invention provides a 1-phenyl-1,2,10,10a-tetrahydropyrrolo[1,2-b]isoquinolin-3(5H)-one compound, a preparation method and an application thereof. The structural formula of the 1-phenyl-1,2,10,10a-tetrahydropyrrolo[1,2-b]isoquinolin-3(5H)-one compound is shown as general formula I, wherein R1 and R2 are selected from hydrogen, methoxy, fluorine, bromine, and hydroxyl, and R1 and R2 are the same or different; R1 is located at any one or two of the ortho, meta, and para positions of the benzene ring, and R2 is located at the 7-position and / or 8-position of the benzene ring; including the formed cis-trans isomers. Through the screening studies on the NMDA-induced PC12 cell injury protection activity and the antidepressant activity in mice, it is shown that the compounds of the present invention can be used for the preparation of drugs for neurodegenerative diseases, especially depression.

The invention discloses a preparation method of a sulfentrazone intermediate, which comprises the following step: in a solvent, carrying out substitution ring closing reaction on a cis-trans isomer compound (nitrohydrazone) as shown in a formula II and cyanate to generate the sulfentrazone intermediate as shown in a formula III. According to the scheme provided by the invention, the problems that a large amount of solid waste is generated and the atom economy is low due to the fact that a large amount of inorganic reducing agents such as sodium sulfite are used in the production of the raw material phenylhydrazine in the process of preparing the triazolinone from the phenylhydrazine in the prior art are solved; the method solves the problem that a large amount of mixed salt is generated due to the fact that a large amount of sulfite reducing agent and sodium hypochlorite oxidizing agent are used in the production of the raw material phenylhydrazine in the process of preparing triazolinone from phenylhydrazine in the prior art, and also solves the problem of low chlorination selectivity of sulfentrazone prepared from phenylhydrazine as the raw material. The problems that diazonium salt is reduced into hydrazine and hydrazine is oxidized into hydrazine are solved; according to the method, intermediate-state hydrazine is directly utilized, and then cyanate is utilized for ring closing, so that compared with the prior art, three steps are shortened, and the energy consumption and the labor intensity are greatly reduced.

The invention discloses a non-destructive testing method for cis-trans-diethyl cyclomalonate based on feature screening, and relates to the technical field of testing or analyzing materials by means of measuring chemical or physical properties of the materials. Comprising the following steps: respectively taking a plurality of mixtures formed by a cis-isomer and a trans-isomer of diethyl cyclomalonate under various mass ratios, and taking Raman characteristic peak parameters of the plurality of mixtures; based on the Raman characteristic peak parameters of the plurality of mixtures, constructing a plurality of Raman characteristic peak proportion variables between cis-trans isomers; constructing a prediction model; on the basis of the prediction model, predicting the mass ratio of cis-trans isomers of the diethyl cyclomalonate; the method has the advantages of rapidness, no damage, no need of separation, high recognition precision and the like, and is suitable for the fields of isomer component analysis, structure identification, precise quantitative detection and the like.

The present invention relates to hydroxamic acid or hydrazide derivatives with nematicidal activity, as well as methods for their preparation and use. Specifically, the present invention discloses a compound represented by formula (I) or its optical isomers, cis-trans isomers, or pesticide-acceptable salts, and methods for their preparation. The compounds of the present invention have excellent nematicidal activity. #imgabs0#

The application discloses a lipoic acid derivatized ceramide and a synthesis method and application thereof, and relates to the technical field of biological medicines. The ceramide has the structure of a general formula I or the structure of an isomer of the general formula I; the isomer includes enantiomers, diastereoisomers and cis-trans isomers. The lipoic acid is reacted with a sphingosine, a phytosphingosine, a dihydrosphingosine and 6-hydroxysphingosine to obtain a novel ceramide. The application introduces a lipoic acid fragment with better solubility, thereby improving the solubility of the ceramide. The ceramide has an obvious promoting effect on the expression of catalase and can effectively prevent the generation of free radicals, thereby having good antioxidant effect.

The invention relates to a 3-thiotetronic acid derivative as well as a preparation method and application thereof. Specifically, the invention provides a compound as shown in a formula (I), and an optical isomer, a cis-trans isomer or a pharmaceutically acceptable salt thereof. The compound provided by the invention has obviously improved insecticidal and acaricidal activity, and can be used for developing novel crop insecticidal and acaricidal agents with low toxicity, high efficiency and environmental friendliness.